Clonagem - phage display
Clonagem e expressão de fragmentos funcionais(scFv) de anticorpo contra o Parvovírus canino-2 com a técnica da Phage display
... (Phage display) tem diversas aplicações, dentre elas a apresentação de fragmentos recombinantes de anticorpos funcionais (scFv), que podem ser produzidos em grande ...utilizou Phage Display ...
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Clonagem e expressão de fragmentos de anticorpos (scFV) contra o vírus da leucemia felina (FeLV) por phage display
... The feline leukemia virus (FeLV) is a retrovirus that infects primarily young cats. In animal clusters, FeLV infection is the largest contributor to mortality. The use of molecular techniques for viral detection has ...
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Caracterização de moléculas envolvidas no desenvolvimento de melanoma pela metodologia de Phage display
... de clonagem, já que há inserção de fragmentos de DNA em regiões não essenciais do genoma do fago, que resultam em partículas virais mais longas contendo uma cópia do inserto de DNA na forma de fita ...
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DESENVOLVIMENTO DE APLICAÇÕES TECNOLÓGICAS DA METODOLOGIA DE PHAGE DISPLAY NO DIAGNÓSTICO DO CÂNCER DE PRÓSTATA
... em clonagem de fragmentos de DNA codificante de milhares de variantes de certos ligantes (ex: peptídeos, proteínas ou fragmentos destas) no interior do genoma de fagos, fusionado ao gene codificante de uma das ...
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CONSTRUÇÃO DE UMA BIBLIOTECA DE ANTICORPOS MONOCLONAIS APRESENTADOS EM FAGOS PARA SELEÇÃO E CARACTERIZAÇÃO DE scFv LIGANTE A PROTEÍNAS INTESTINAIS DE DIATRAEA SACCHARALIS Aluno: Rafael Nascimento
... de Phage-Display (Ph.D.) (McCafferty et al., 1990), Ribossome Display (Hanes e Pluckthun, 1997; He e Taussig, 1997) e Cell-Surface Display (Francisco et ...e clonagem de diversos genes ...
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Selection of specific colon cancer tumor peptides from combinatorial phage display libraries
... of phage display affinity ...the phage displaying peptides that did not bind to the target ...fittest phage clones over the background and for this reason the washes need to be less stringent ...
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Sheep polyclonal antibody to map Haemonchus contortus mimotopes using phage display library
... two phage clones containing peptides with homology to GAPDH (clone 6) and Dim 1 (clone 17) proteins Figure ...M13 phage (a), Clone 6 (b), Clone17 (c), Chemically-synthesized GAPDH mimotope (d) and ...
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Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library
... in phage display ...scFv phage display library constructed from blood from melanoma patients and the other using an Fab library, the authors were able to isolate only one positive scFv and ...
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Boophilus microplus cathepsin L-like (BmCL1) cysteine protease: Specificity study using a peptide phage display library
... The tick Rhipicephalus (Boophilus) microplus is one of the most important bovine ectopar- asites, a disease vector responsible for losses in meat and milk productions. A cysteine protease similar to cathepsin L, named ...
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Identificação e caracterização de peptídeos bioativos através de Phage display usando genoma completo de Corynebacterium pseudotuberculosis
... tool Phage Display was used that selects peptides using bacteriophages to express random sequences of peptides on their ...surface. Phage Display has been used successfully in many ...
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Identification and rational redesign of peptide ligands to CRIP1, a novel biomarker for cancers.
... of phage display and computational molecular modeling to identify a high-affinity ligand for ...CRIP1. Phage display panning experiments initially identified consensus peptide sequences with ...
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Tristetraprolin inhibits poly(A)-tail synthesis in nuclear mRNA that contains AU-rich elements by interacting with poly(A)-binding protein nuclear 1.
... a phage-display biopan using TTP as ...the phage library protein fragments that bound TTP (Table S1) are those for poly(A)-binding protein nuclear 1 (PABPN1), which binds to poly(A) tails of nuclear ...
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SELEÇÃO DE PEPTÍDEOS RECONHECIDOS POR IMUNOGLOBULINAS Y ANTI-METACESTÓDEO DE Taenia solium POR PHAGE DISPLAY
... técnica phage display visa à caracterização molecular de ...phage display. Estes autores relataram a seleção de peptídeos por phage display como uma ferramenta para o ...
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Identification of human embryonic progenitor cell targeting peptides using phage display.
... the phage display approach described here to isolate stem cell targeting peptides that would facilitate retrieval of the equivalent cells from patient derived iPS cells to provide a source of genetically ...
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Screening of a specific peptide binding to VPAC1 receptor from a phage display peptide library.
... value, and it bound more effectively than any of the other clones (Figure 3). Therefore, the phage clone VP2 and its displaying peptide were selected for further investigation. A multiple sequence alignment showed ...
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Desenvolvimento de uma vacina sintética contra a Leishmaniose Visceral Canina utilizando tecnologia peptídica (Phage Display e Spot Síntese)
... chagasi (azul) foram capazes de induzir anticorpos reativos contra o próprio imunógeno utilizado, enquanto que os soros dos animais imunizados com os peptídeos 11H[r] ...
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Identification of Fusarium virguliforme FvTox1-Interacting Synthetic Peptides for Enhancing Foliar Sudden Death Syndrome Resistance in Soybean.
... Like single variable fragment plant antibodies created based on mammalian mRNA mole- cules, linear peptides also have the ability to specifically bind and alter functions of target pro- teins. Compared to macromolecular ...
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A broad set of different llama antibodies specific for a 16 kDa heat shock protein of Mycobacterium tuberculosis.
... by phage display, recombinant variable domains of heavy-chain antibodies (VHH) binding to ...Two phage display selection strategies were followed: one direct selection using semi-purified ...
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Selection of binding targets in parasites using phage-display and aptamer libraries in vivo and in vitro
... using phage display are directed toward this ...a phage display library was used to screen for peptides with in vitro activity against ...
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Discovery and Characterization of Phage Display-Derived Human Monoclonal Antibodies against RSV F Glycoprotein.
... 2E1 neutralized RSV A Long strain more potently than RSV B Washington strain. The four residues that we identified from shotgun mutagenesis as critical for 2E1 binding are all con- served between these two strains. When ...
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