cysteine protease
In vitro ANTIGIARDIAL ACTIVITY OF THE CYSTEINE PROTEASE INHIBITOR E-64
... As many efforts have been made to search new alternatives for Giardia infection treatment, the assessment of a drug potential requires suitable models that allow identifying the biological processes on which they can ...
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A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus
... the cysteine protease ...that cysteine proteases from several plant species act on the protective cuticle of adult nematodes, degrading this structure and rendering it weak enough to be ruptured ...
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Tigutcystatin, a cysteine protease inhibitor from Triatoma infestans midgut expressed in response to Trypanosoma cruzi
... papain-like cysteine protease, cruzi- pain, to survive and infect hosts [15,16]; however, this protozoan is not pathogenic for triatomine bugs ...Therefore, cysteine pro- tease inhibitors from ...
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Boophilus microplus cathepsin L-like (BmCL1) cysteine protease: Specificity study using a peptide phage display library
... The tick Rhipicephalus (Boophilus) microplus is one of the most important bovine ectopar- asites, a disease vector responsible for losses in meat and milk productions. A cysteine protease similar to ...
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Unfolding Studies of the Cysteine Protease Baupain, a Papain-Like Enzyme from Leaves of Bauhinia forficata: Effect of pH, Guanidine Hydrochloride and Temperature
... Protein unfolding induced by chemical denaturants, pH or temperature are common approaches to study proteins in vitro. Here, we investigated the conformational behavior of baupain in solution as a function of pH, ...
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Heparin modulates the endopeptidase activity of Leishmania mexicana cysteine protease cathepsin L-Like rCPB2.8.
... secreted cysteine protease (CPB) can participate in Leishmania infection by degradation of fibronectin of the host’s extracellular matrix (ECM), thereby facilitating the local spread of the parasite ...
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Identification, Characterization and Down-Regulation of Cysteine Protease Genes in Tobacco for Use in Recombinant Protein Production.
... An in silico analysis was performed using the DFCI tobacco gene index (TGI) database that contains 324,058 tobacco expressed sequence tags (ESTs). A total of 55 putative CysP genes were identified in the database using ...
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A cysteine protease inhibitor of plasmodium berghei is essential for exo-erythrocytic development.
... parasites could not be recovered from infected mosquitoes [30]. This result led to the hypothesis that Plasmodium ICPs might only be essential for development of the parasite in the mosquito. In a recent study describing ...
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Chemoenzymatic synthesis of organoselenium(IV) compounds and their evaluation as cysteine protease inhibitors
... Organoselenanes showed both time-dependent and concentration-dependent behavior on the inhibition of cathepsins S and V, as observed in Figure 5A. Dibromo organoselenanes (2, 5 and 6) were found to inhibit both enzymes ...
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Rmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune response
... The nucleotide sequence of Rmcystatin-3 (R. microplus cystatin- 3) was identified in a hemocyte cDNA library of bovine tick. The sequence showed a 426-bp-ORF and an amino acid sequence characteristic of the family 2 ...
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Dual function of novel pollen coat (surface) proteins: IgE-binding capacity and proteolytic activity disrupting the airway epithelial barrier.
... The cloned cDNA encodes a protein that significantly matches the peptide sequencing results of the native IgE-binding CP generated by mass spectrometry and Edman degradation cyclohexane. Cyclohexane-soluble proteins ...
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The bacterial effector HopX1 targets JAZ transcriptional repressors to activate jasmonate signaling and promote infection in Arabidopsis.
... Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes a suite of virulence effector proteins into the host cytoplasm, and the production of a number of toxins such as ...
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Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli
... Sequences were edited to remove the vector sequence and the extra restriction sites. One open reading frame (ORF) was obtained using the ORF finder at the National Center for Biotechnology Information (NCBI) website. The ...
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Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin.
... Eh (HM1:IMSS; ATCC) and EhCP5 - (a gift from D. Mirelman; Weizmann Institute of Science) trophozoites were cultured in TYI-S-3 medium containing 100U/mL penicillin/streptomycin sulfate at 37°C in sealed borosilicate ...
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Srv mediated dispersal of streptococcal biofilms through SpeB is observed in CovRS+ strains.
... the cysteine protease SpeB, as biofilm formation was restored through either chemical inhibition of SpeB or allelic replacement of speB in the MGAS5005Dsrv background in both in vitro and in vivo biofilm ...
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Cathepsin inhibition-induced lysosomal dysfunction enhances pancreatic beta-cell apoptosis in high glucose.
... lysosomal protease inhibitors in 11 mM glucose (normal culture concentration) medium for 48 hr, and then the activation of apoptosis was analyzed by western blotting with antibodies against the active, cleaved ...
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Protease Gene Shuffling and Expression in Pichia pastoris
... The purified PCR product was ligated with pMD19-T vector and transformed into E. coli competent cells by heat shock method, and the transformants were selected on LB plates containing 0.4% casein, ampicillin (100 mg/mL), ...
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MATERIALS AND METHODS Microorganism
... alkaline protease (130 U/ ml) was produced during the early stationary ...produce protease with 0-10% NaCl (w/v), the optimum being 5% NaCl ...and protease production was optimum at pH 9 with ...
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Different feeding habits influence the activity of digestive enzymes in freshwater fish
... ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species’ ...
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Protease-sensitive synthetic prions.
... 3. Protease-sensitive synthetic prions are serially transmissible in Tg9949 ...no protease-resistant PrP by Western blotting (A), but activity in the ASA (B) and neuropathology consistent with prion disease ...
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