DNA methylation analysis
Genome-wide DNA methylation analysis reveals a potential mechanism for the pathogenesis and development of uterine leiomyomas.
... Paired specimens of leiomyoma and adjacent normal myome- trium were obtained from 10 Japanese women who had a single leiomyoma nodule to limit biological heterogeneity (Table 1). The women underwent hysterectomy, and ...
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Genome-wide DNA methylation analysis predicts an epigenetic switch for GATA factor expression in endometriosis.
... of DNA methylation on gene ...CpG methylation in a site-specific manner prevents us from unambiguously interrogating the direct effects of these epigenetic ...multiple methylation differences ...
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DNA methylation analysis of the angiotensin converting enzyme (ACE) gene in major depression.
... intensive DNA methylation in the ( 2456/2255) ACE gene region are consistent with a recent report by Rivie`re et ...of DNA methylation and chromatin condensation state on the expression of the ...
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Genome-Wide DNA Methylation Analysis Identifies Novel Hypomethylated Non-Pericentromeric Genes with Potential Clinical Implications in ICF Syndrome.
... aberrant methylation in ICF patients were almost exclusive of pericentro- meric regions of chromosome 1, 16, sometimes 9 and associated to repeated DNA sequences or heterochromatic ...genome-wide DNA ...
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DNA methylation analysis of the tumor suppressor gene CDKN2B
... Bisulfite DNA modification was accomplished in agreement with the technique described in Current Proto- cols In Human Genetics (Dracopoli et ...1994). DNA (2 µg) was denatured with NaOH (2 M) for 10 min at ...
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DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level.
... average methylation levels within 7 different D4Z4 regions, at single nucleotide ...genomic DNA was amplified with bisulfite-converted primers and PCR products were cloned and ...genomic DNA ...
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Genome-wide DNA methylation patterns and transcription analysis in sheep muscle.
... DNA methylation plays a central role in regulating many aspects of growth and development in mammals through regulating gene ...resolution analysis of DNA methylation landscapes using ...
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Roadmap of DNA methylation in breast cancer identifies novel prognostic biomarkers
... Genome-wide DNA methylation analysis reveals 368 differentially methylated CpG sites in breast cancer tissue We set out to investigate the genome-wide DNA methy- lation profiles in a panel of ...
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Global DNA methylation of ischemic stroke subtypes.
... in DNA methylation contribute to inter-individual phenotypic variation and are associated with cancer development and other complex diseases ...blood DNA, which has been associated with age, sex, ...
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Normal DNA methylation dynamics in DICER1-deficient mouse embryonic stem cells.
... Genomic DNA isolated from the SC and MC lines was assayed for DNA methylation at MinS ...in methylation was seen in the two SC lines (Figure 5E), and these were not analysed ...of DNA ...
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Comprehensive analysis of gene expression and DNA methylation data identifies potential biomarkers and functional epigenetic modules for lung adenocarcinoma
... challenging. DNA methylation changes have been reported to occur early in carcinogenesis (Teschendorff et ...and DNA methylation analysis seems to be a promising strategy in cancer ...
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Androgen receptor function links human sexual dimorphism to DNA methylation.
... variability list – despite the fact that this list is not significantly enriched for AR targets - have documented roles in androgen2/ genital tissue-related biology. These genes include (I) genes linked to ...
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Deciphering DNA methylation under heat stress in contrasting rice genotypes
... do DNA ao nível global através de duas técnicas nomeadamente, a imunodetecção com um anticorpo para a 5-metilcitosina de locais de metilação em núcleo interfásicos de secções de tecido da raíz de arroz e a ...
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DNA methylation profile in patients with hepatocellular carcinoma - Artigo de investigação médica
... DAPK methylation were observed between tumor and non-tumor tissue, being methylated in almost 50% of the ...DAPK methylation in non-malignant cirrhotic liver and in normal liver ...
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Estimation of the fraction of cancer cells in a tumor DNA sample using DNA methylation.
... Figure 1. Selection processes of specific genomic regions. (A) The concept of estimation of the fraction of cancer cells in a tumor DNA sample using DNA methylation. (B) Selection of specific genomic ...
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Disclosing bias in bisulfite assay: MethPrimers underestimate high DNA methylation.
... non-CpG) methylation to ensure that cytosine conversion was ...the methylation patterns we found were ...genomic DNA as template with the same MIPs primers used for bisulphite PCR; the same purified ...
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Morphologi cal analysis and DNA methylation in
... Even with little genetic diversity, the phenotypes of C. bonariensis showed differences in several morphological characteristics. Similar results were also found by Richards et al. (2008), in Fallopia japonica (Houtt.) ...
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Chromosome and DNA methylation dynamics during meiosis in autotetraploid Arabidopsis arenosa
... In diploid A. thaliana, as in other Arabidopsis species, interphase chromatin organization is characterized by the presence of chromocenters cytologically defined as con- densed chromatin domains that display strong DAPI ...
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In vitro analysis of integrated global high-resolution DNA methylation profiling with genomic imbalance and gene expression in osteosarcoma.
... IN DNA including the whole-genome amplification, DNA fragmentation, labeling, hybridization, scan- ning and software ...of analysis gene-specific real-time PCR quantitation of the IP enriched ...
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DNA methylation regulates expression of VEGF-C, and S-adenosylmethionine is effective for VEGF-C methylation and for inhibiting cancer growth
... by DNA methylation and histone modification rather than changes in DNA ...sequence. DNA methylation plays a critical role in regulating and reprogramming gene expression patterns in ...
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