DNA sequencing
Massively parallel DNA sequencing facilitates diagnosis of patients with Usher syndrome type 1.
... exon sequencing of selected genes using the Massively Parallel DNA Sequencing (MPS) technology would enable us to systematically tackle previously intractable monogenic disorders and improve ...
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Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system
... molecule sequencing technologies are being developed to address this ...the sequencing error rate is by this ...(SMRT) DNA Sequencing System ligates hairpin adapters ...double-stranded ...
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CNVkit: Genome-Wide Copy Number Detection and Visualization from Targeted DNA Sequencing.
... targeted DNA sequencing data that we packaged into a software ...off-target sequencing reads and applies a series of corrections to improve accu- racy in copy number ...
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DNA extraction, PCR, and DNA sequencing
... o DNA Barcode em novas áreas para estabelecer novos registros de Liriomyza sativae no Brasil e também a determinação da diversidade genética intra e interpopulacional, e reconstruir a filogenia das espécies ...
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A Rapid Method for Cannabis Species Determination by DNA Sequencing
... of DNA (due to the high copy number of rRNA genes), and b) has a high degree of variation even between closely related species (Coleman, 2007; Shultz et ...
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Exonic DNA sequencing of ERBB4 in bipolar disorder.
... for sequencing the 189 subjects from the GAIN BP sample who had a lifetime history of mood- incongruent psychosis as previously defined ...and DNA for one subject was unavailable, leading to a final count ...
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Amplification, cloning and DNA sequencing of the
... A range of Web-based tools were used for analysis of DNA and protein sequences. Lactate dehydrogenase sequences of apicomplexan parasites and the host B. taurus were obtained from National Centre for Biotechnology ...
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Clinics vol.61 número2
... by DNA sequencing analysis of fragments amplified by polymerase chain reaction (PCR) from 3 different Cryptosporidium loci: the SSUrRNA coding region, the Cryptosporidium oocyst wall protein (COWP) gene, ...
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Analysis of RAD sequencing data from species of Mediterranean cicadas
... SMRT sequencing is a parallelized sequencing method that utilizes SMRT cells with a lot of zero-mode ...single DNA polymerase that is affixed at its bottom with a single molecule of DNA as the ...
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Mycobacterium tuberculosis MLPA assay versus Genotype
... DNA sequencing procedures - The key genomic re- gions involved in the INH resistant phenotype (katG and inhA genes) and RIF resistant phenotype (rpoB gene) were amplified and sequenced according to ...
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Pyrosequencing-An Alternative to Traditional Sanger Sequencing
... efficient DNA sequencing techniques for sequence ...in DNA sequencing and analysis of polymorphic DNA fragments in many clinical and research ...of DNA sequencing ...
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Genetic diversity of medically important and emerging Candida species causing invasive infection
... of DNA sequencing as a gold stand- ard method for yeast identification by clinical laboratories has been hindered by several factors, including its cost, the lack of well-trained professionals [7], the ...
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An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates
... the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA), the recombination repair protein (recA), the RNA polymerase alpha subunit (rpoA) and the excision repair ...
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Detection of tuberculosis drug resistance a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype MTBDRplus
... DNA sequencing procedures - The key genomic re- gions involved in the INH resistant phenotype (katG and inhA genes) and RIF resistant phenotype (rpoB gene) were amplified and sequenced according to ...
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Ultra-deep sequencing of mouse mitochondrial DNA: mutational patterns and their origins.
... next-generation sequencing of the human mitochondrial genome have identified a small number of sites, in which normal individuals carry both the wildtype copy and a high-frequency mutant allele ...a DNA ...
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Detection of genomic variation by selection of a 9 mb DNA region and high throughput sequencing.
... We also observed that regions of low-coverage are associated with low-complexity DNA. We hypothesized that this phenome- non may be an artifact resulting from the inability to uniquely map short Illumina reads ...
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High-Specificity Targeted Functional Profiling in Microbial Communities with ShortBRED.
... metagenomic sequencing data by one of three approaches: (i) mapping DNA reads to a database of nucleotide sequences [1], (ii) mapping translated DNA reads to a database of protein sequences [2], or ...
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TOX3 mutations in breast cancer.
... TOX3 maps to 16q12, a region commonly lost in breast cancers and recently implicated in the risk of developing breast cancer. However, not much is known of the role of TOX3 itself in breast cancer biology. This is the ...
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Trichloroethylene-induced gene expression and DNA methylation changes in B6C3F1 mouse liver.
... and DNA methylation changes in the liver of B6C3F1 mice orally administered with TCE (0, 100, 500 and 1000 mg/kg ...of DNA methylation, such as Utrf1, Tet2, DNMT1, DNMT3a and DNMT3b, were ...global ...
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Classification of dermatophytes by a multilocus phylogenetic approach based on tef-1α, beta tubulin and its genes
... 24 one can expect the amount of DNA eluted during the final step to be fairly consistent between samples. The instrument bases sample transfer on magnetic handling, problems normally associated with clogged tips, ...
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