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Enzymes - Purification

Extraction and Purification of Collagenase Enzymes: A Critical Review

Extraction and Purification of Collagenase Enzymes: A Critical Review

... statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low ...useful ...

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Production, purification, cloning and application of lytic enzymes.

Production, purification, cloning and application of lytic enzymes.

... PRODUCTION, PURIFICATION, CLONING AND APPLICATION OF LYTIC ENZYMES: Lytic enzymes such as β-1,3 glucanases, proteases and chitinases are able to hydrolyse, respectively, β-1,3 glucans, mannoproteins ...

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Purification and functional characterization of two fibrinogenolytic enzymes from Bothrops alternatus venom

Purification and functional characterization of two fibrinogenolytic enzymes from Bothrops alternatus venom

... Both enzymes were proteolytically active against bovine fibrinogen as ...the enzymes was inhibited by 1,10-phenanthroline, suggesting they are ...both enzymes were found to cause defibrinogenation ...

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Biopharmaceuticals from microorganisms : from production to purification

Biopharmaceuticals from microorganisms : from production to purification

... Magnetic separation with immunocapture supports stands among the techniques used in purification kits, but just recently its application to industrial scale showed viable paths. The initial high costs of the beads from ...

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RESULTS AND DISCUSSION Purification of Lipase

RESULTS AND DISCUSSION Purification of Lipase

... Lipases are glycerol ester hydrolases (EC 3.1.1.3), which hydrolyze ester linkages of glycerides at water-oil interface (5,34,44). An important characteristic of lipases is their ability not only to hydrolyze the ester ...

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Partial Purification of Milk Clotting Protease

Partial Purification of Milk Clotting Protease

... Vegetable rennet extracted from Cynara cardunculus L. (cardoon flowers) is used for various cheese productions, such as La Serena cheese, a semi-hard Spanish variety manufactured from raw merino ewes’ milk. Aqueous ...

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Glycanzyme: characterisation of novel enzymes for protein glycoengineering

Glycanzyme: characterisation of novel enzymes for protein glycoengineering

... these enzymes. Therefore, improvements in their production and purification will still be required to generate enough protein for activity studies and eventual ...protein. Purification from inclusion ...

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Antibodies purification using centrifugal partition chromatography

Antibodies purification using centrifugal partition chromatography

... substances: enzymes, like lysozyme, to digest the bacterial walls; peptides, like defensins, that lyse the bacterial cell membranes directly; and the plasma proteins of the complement system responsible for the ...

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Magnetic Purification Of Antibodies

Magnetic Purification Of Antibodies

... and enzymes which are sensitive molecules for temperature as well as processing conditions responsible for making processing more difficult [3] ...Various purification steps that were used contain ...

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Purification of Konjac Glucomannan for Tissue Engineering Applications

Purification of Konjac Glucomannan for Tissue Engineering Applications

... the purification methodologies applied in this work did not introduced significant changes on the structure of the ...The purification of the polymer by filtration through ...Additional purification ...

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Monoliths for purification of a DNA vaccine against Influenza

Monoliths for purification of a DNA vaccine against Influenza

... The purification step aims the separation of the biologically active supercoiled (sc) pDNA from the other isoforms, together with the removal of all the impurities ...pDNA purification, most of them based ...

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Simplified Method for Rapid Purification of Soluble Histones

Simplified Method for Rapid Purification of Soluble Histones

... heterodimers and H3H4 form heterotetramers. Therefore, there is no special need for purification of the individual his- tones. When compared to preparation of the individual his- tone protein, co-expression and ...

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Biogas production and purification using membrane processes

Biogas production and purification using membrane processes

... Biogas production and purification using membranes processes 44 Through the analysis of Figure 3.10 is possible to observe that all prepared membranes presented a similar profile up to 200ºC. Besides, were not ...

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C-PHYCOCYANIN PURIFICATION: MULTIPLE PROCESSES FOR DIFFERENT APPLICATIONS

C-PHYCOCYANIN PURIFICATION: MULTIPLE PROCESSES FOR DIFFERENT APPLICATIONS

... the purification may possibly be due to lower membrane polarization caused by buffer addition at the beginning of the process, as was identified by Li et ...

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Hybrid gravitational microfiltration system for drinking water purification

Hybrid gravitational microfiltration system for drinking water purification

... water purification can aid the development of new technologies appropriate for different regions of the ...water purification can help to provide cost-effective and robust solutions (Shannon et ...

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Hybrid magnetic systems for the purification of biopharmaceuticals

Hybrid magnetic systems for the purification of biopharmaceuticals

... In the last chapter, the MNPs were applied in the PEG-NaPA systems to study their partitioning behavior and their potential to recover BSA and IgG, with aim to find a suitable magnetic aqueous two phase fishing system ...

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Molecular characterization of genes encoding trypsin-like enzymes from Aedes aegypti larvae and identification of digestive enzymes

Molecular characterization of genes encoding trypsin-like enzymes from Aedes aegypti larvae and identification of digestive enzymes

... The digestive trypsins of several insects were purified by classical chromatographic methods, among them the trypsins of insect species like Periplaneta americana (Lopes and Terra, 2003), Locusta migratoria (Lam et al., ...

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PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY

PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY

... The objective of this study was to investigate the use of prickly pear cv. Miúda (Nopalea cochenillifera (L.) Salm Dyck) as the main raw material for the production of the enzymes endoglucanase (CMCase) and ...

12

Antibodies purification using aqueous micellar two-phase systems

Antibodies purification using aqueous micellar two-phase systems

... IgY purification in the micelle-poor ...IgY purification and recovery are presented in Figures ...IgY purification since the amount of proteins added to the systems is also ...

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Collagenolytic enzymes produced by fungi: a systematic review

Collagenolytic enzymes produced by fungi: a systematic review

... new production methods, including the search for producing micro-organisms, alternative sources of substrates, and bet- ter extraction conditions and purification of collagenase, has been of great importance, since it has ...

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