heterologous expression
Heterologous expression in Caenorhabditis elegans as an alternative approach to functional studies in Schistosoma mansoni
... all heterologous expression system, one must be careful when extrapolating data, par- ticularly in the functional analysis of dis- tantly related species, which genes con- served in sequence level may be ...
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Heterologous expression of Ralp3 in Streptococcus pyogenes M2 and M6 strains affects the virulence characteristics.
... The heterologous expression of ralp3 in both serotypes resulted in reduced attachment to and internalization into the majority of tested epithelial ...ralp3 expression strains showed a decreased ...
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Improved cellulolytic efficacy in Penicilium decumbens via heterologous expression of Hypocrea jecorina endoglucanase II
... by heterologous expression of endoglucanase II from ...jecorina. Expression of Hjegl2 in ...gous expression of the Hjegl2 gene. he expression studies did, however, conirm the inductive ...
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Fungal His-tagged nitrilase from Gibberella intermedia: gene cloning, heterologous expression and biochemical properties.
... nitrilase superfamily. As previously reported, nitrilase contained the invariant catalytic triad residues, glutamate, lysine and cysteine as one of the members in the nitrilase superfamily [26]. The amino acid residues ...
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Isolation of dihydroflavonol 4-reductase cDNA clones from Angelonia x angustifolia and heterologous expression as GST fusion protein in Escherichia coli.
... Although the two cDNA clones differed only in three amino acids, no functional activity could be observed with recombinant Ang.DFR1. To identify the decisive residues, we mutated the two DFR cDNA clones by site-directed ...
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Hypericum perforatum phenolic compounds : protective role in the toxicity induced by heterologous expression of α-synuclein and huntingtin in yeast cells
... the heterologous expression of the protein reduced the cell survival to values of ...protein expression, ...the heterologous expression reduces the cell survival to values of ...by ...
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Xenobiotic Compounds Degradation by Heterologous Expression of a Trametes sanguineus Laccase in Trichoderma atroviride.
... for heterologous expression of proteins because they can carry out eukaryotic posttranslational modifications and show good secretion capacities since they are efficient producers of extracellular enzymes ...
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Impact of cell type and epitope tagging on heterologous expression of G protein-coupled receptor: a systematic study on angiotensin type II receptor.
... Despite heterologous expression of epitope-tagged GPCR is widely adopted for functional characterization, there is lacking of systematic analysis of the impact of expression host and epitope tag on ...
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Heterologous expression of wheat VERNALIZATION 2 (TaVRN2) gene in Arabidopsis delays flowering and enhances freezing tolerance.
... the expression level of several genes involved in flowering time regulation was ...of expression of the major flowering control genes, FCA, FLC, FT, FVE and ...that heterologous expression of ...
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Heterologous expression of the AtDREB1A gene in transgenic peanut-conferred tolerance to drought and salinity stresses.
... AtDREB1A, a class of DREB from Arabidopsis thaliana, recognizes dehydration responsive elements/C-repeat elements (DRE/CRT) of the promoters of many downstream stress-inducible genes under various abiotic stresses [19, ...
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Heterologous Expression of Pantoea Agglomerans Phytase Gene Optimized for Plant-Host Expression
... ccatgggccaagaaactcctgaaggatatc-3’ and 5’- ctcgagcttttcaaattgaggatgagaccacttagcagcattattc- 3’ with the following PCR conditions: one cycle at 98°C for 2 min, followed by 37 cycles of 98°C for 25 s, 57°C for 25 s, and 72°C ...
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Heterologous expression, protein folding and antibody recognition of a neurotoxin from the Mexican coral snake Micrurus laticorallis
... recombinant expression over chemical synthesis seems to be a reliable approach to obtain sufficient amounts of Mlat1 for animal ...a heterologous ex- pression system for obtaining recombinant HisrMlat1 or ...
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Heterologous expression and biochemical characterization of an α1,2-mannosidase encoded by the Candida albicans MNS1 gene
... ods. Cells grown in the presence of 1.0 mM IPTG ex- pressed a 68 kDa polypeptide whose intensity increased as a function of the induction time (Fig. 1), although no further increase in induction was observed after 3 h of ...
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Heterologous Expression in Remodeled C. elegans: A Platform for Monoaminergic Agonist Identification and Anthelmintic Screening.
... the heterologous, ectopic expression of potential key drug targets from parasitic nematodes, for use as a platform for agonist identification and potential anthelmintic ...the expression of nematode ...
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Heterologous Expression of a Trypanosoma cruzi Surface Glycoprotein (gp82) Indicates that Requirements for Glycosylphosphatidylinositol Anchoring are Different in Mammalian Cells and this Trypanosome
... Replacement of aspar- tic acid at site ω by serine, or of serine at site ω +2 by alanine, converts the inactive VSG GPI signal to viable signal for mammalian cells (Moran & Caras [r] ...
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Heterologous expression of human KRASwt cDNA in Saccharomyces cerevisiae and its mutants from the Ras signalling pathway and phenotype screening
... Ras2 is expressed with roughly equal efficiency in any carbon source, whereas Ras1 is highly expressed on glucose and poorly expressed in non-fermentable carbon sources. As consequence, the ras2∆ strain is defective on ...
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Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system
... Heterologous expression could lead to intracellular accumulation, aggregation, or degradation of the protein in the cytoplasm ...nuc expression and secretion sig- nals and it is able to secrete ...
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Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host.
... for heterologous expression in ...the expression of the codon harmonized gene sequences for three additional malaria vaccine candidates, namely MSP1 42 (FVO), MSP1 42 (3D7) and MSP1 42 ...
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Engineering transcriptional regulation to control Pdu microcompartment formation.
... LB, expression of PocR by addition of 1 ng/mL aTc resulted in increased fluorescence even in the absence of 1,2-PD, while strains carrying a negative control vector encoding for maltose-binding protein (MBP) ...
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Expressão e caracterização de uma protease de interesse biotecnológico clonada da...
... the expression of a serine protease from Crotalus durissus collilineatus (Collineina- 1) in Pichia pastoris, the purification of the native toxin from ...resistance. Heterologous expression was ...
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