Micronucleus assay
Evaluation of genotoxicity using the micronucleus assay and nuclear abnormalities in the tropical sea fish Bathygobius soporator
... The micronucleus and nuclear abnormalities assays have been used increasingly to evaluate genotoxicity of many compounds in polluted aquatic ...the micronucleus as- say and nuclear abnormality assay ...
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Cytogenetic abnormalities in lymphocytes evaluated with micronucleus assay in medical personnel occupationally exposed to ionizing radiation
... The aim of this study was to evaluate the genotoxicity of ionizing radiation on medical personnel using the micronucleus assay and to determine the human health risk. Paired Student’s t-test shows ...
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Micronuclei induced by reverse transcriptase inhibitors in mononucleated and binucleated cells as assessed by the cytokinesis-block micronucleus assay
... In this study, we investigated the clastogenic and/or aneugenic potential of AZT, 3TC and d4T using the cyto- kinesis-block micronucleus (CBMN) assay in human lym- phocyte cultures. We also examined the ...
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FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2
... Cell Micronucleus Test, the cell count as well as micronucleus is performed by light microscopy, or fluorescence-based microscopy ...MN assay is increasing in several research ...and ...
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Absence of mutagenicity of plants used to treat gastrointestinal disorders
... the micronucleus assay iden- tiies agents that cause DNA breaks or loss of whole chromosomes as the Salmonella typhimurium assay identiies agents that cause point ...
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J. Braz. Chem. Soc. vol.22 número10
... bioactive compounds. This work intended to isolate and describe the constituents of the active fractions and to evaluate their effects. As a result, sixteen compounds were characterized, including a new natural product ...
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Lúcia Maria de Miranda Adad 1,2 , Heloísa Helena Rodrigues de Andrade3
... buccal micronucleus assay, hematological and lipid parameters, butyrylcholinesterase (BChE) activity and genetic polymorphisms of enzymes involved in the metabolism of pesticides, such as PON1, as well as ...
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Evaluation of micronuclei frequency in Tradescantia pallida pollen mother cells treated with ethanolic extracts isolated from Cryptocarya mandioccana, Cryptocarya moschata and Pterogyne nitens
... Although herbal extracts contain several classes of compounds with pharmacological activity, they also present toxic substances with mutagenic effects. The aim of the present study was to verify the mutagenicity of ...
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Redução da genotoxicidade induzida pela doxorrubicina por Handroanthus impetiginosus na medula óssea de camundongos revelada pelo ensaio do micronúcleo
... Healthy, heterogeneous, young adult male and female Swiss albinus (Unib: SW) mice (between 7 and 12 weeks – pubescent period), with a body weight between 30 g and 40 g (i.e., the variation weight between the animals, for ...
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Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo
... (comet assay, micronucleus assay, and aberrant crypt focus assay) and tests 4 protocols of treatment with inulin (pre-treatment, simultaneous, post- treatment, and pre + ...the ...
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Use of 2-color flow cytometry to assess radiation-induced genotoxic damage on CHO-KI cells
... The micronucleus assay is an important technique used to evaluate genotoxic damage of chemical or physical agents (as ionizing radiations) on cells, based on quantification of cells bearing micronuclei, ...
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Avaliação do efeito quimiopreventivo de Brassica oleracea (variedade acephala) e do omeprazol em um modelo experimental de indução do refluxo duodenogastroesofágico
... Aim: To evaluate, in an experimental model, the applicability of erythrocyte micronucleus assay in diagnosis of esophageal damage caused by gastroesophageal reflux [r] ...
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Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system
... respectively. Micronucleus assay showed absolutely no genotoxicity or cytotoxicity effects and histological analysis showed no alterations or even nanoparticle clusters in several investigated organs but, ...
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Assessment of effects of mouthwash by Buccal Micronucleus Cytome Assay: a Preliminary Study
... In our study, the Buccal Cytome Micronucleus Assay was performed in volunteers exposed to mouthwashes with or without alcohol to evaluate in vivo cellular response. The buccal mucosa is a barrier to ...
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Effects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamide
... The micronucleus assay is often used to evaluate the organisms exposure to chemical or physical agents (Tucker and Preston, 1996), being an important procedure in ge- netic toxicology and when evaluating ...
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Investigation on the Genotoxicity of Mercuric Chloride to Freshwater Clarias gariepinus
... observed that micronuclei formations varied significantly with exposure concentrations. The MNT has been employed successfully in various fish species to detect mutagenic changes caused by aquatic pollutants (Pantaleao ...
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Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)
... fications introduced by Klaude et al. (1996) as well as some additional modifications. Briefly, for each mouse periph- eral blood was collected from the orbital vein at 4 h and 24 h post-gavage and a 10 µL aliquot mixed ...
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Rev. Bras. Reumatol. vol.54 número1
... In the 1980s there was a shift to HEp-2 cells as substrate for the assay. This added a whole new dimension to the ANA test due to the fact that it added a higher sensitivity and a vast ar- ray of new immunol ...
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In vitro antimicrobial activity assay
... In current research, nine basic esters of para-alkoxyphenylcarbamic acid with incorporated 4-(4- fluoro-/3-trifluoromethylphenyl)piperazin-1-yl fragment, 6i-6m and 8f-8i, were screened for their in vitro antimicrobial ...
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Histamine release and fluorometric assay
... on the guinea pig lung cell suspension containing mast cells, as well as its influence on the histamine release induced by antigen (ovoalbumin 10 Pg/ml) and ionophore A 23187 (3 PM), i[r] ...
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