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molecular diagnosis

Molecular Diagnosis of Pathogenic Sporothrix Species.

Molecular Diagnosis of Pathogenic Sporothrix Species.

... subsequent molecular diagnosis of sporotrichosis had sensitivity and specificity values that approached 100%; this PCR assay outperformed histological and or/ CFU assays in the detection of ...direct ...

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MOLECULAR DIAGNOSIS OF Guignardia citricarpa IN ASYMPTOMATIC SWEET ORANGE TISSUE

MOLECULAR DIAGNOSIS OF Guignardia citricarpa IN ASYMPTOMATIC SWEET ORANGE TISSUE

... In addition to contributing to plant health, early detection of the pathogen in asymptomatic tissue is essential for the certification of citrus fruits destined for export to countries with stringent phytopathogen ...

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Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis

Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis

... Compared to the traditional PCR protocols, the protocol presented herein allows a better interpretation of PCR re- sults and promote quality assurance of the leishmaniasis diagnosis. The cost-benefit ratio is ...

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Exome sequencing is an efficient tool for variant late-infantile neuronal ceroid lipofuscinosis molecular diagnosis.

Exome sequencing is an efficient tool for variant late-infantile neuronal ceroid lipofuscinosis molecular diagnosis.

... progression. Molecular genetics has emerged in recent years as a useful tool for enhancing NCL subtype ...the diagnosis of neurodegenerative disorders mutations in these genes may cause similar phenotypes, ...

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Molecular diagnosis of Anaplasmataceae organisms in dogs with clinical and microscopical signs of ehrlichiosis

Molecular diagnosis of Anaplasmataceae organisms in dogs with clinical and microscopical signs of ehrlichiosis

... Co-infections with more than one ehrlichiosis agent have been reported in dogs (BREITSCHWERDT et al., 1998; KORDICK et al., 1999). The clinical signs of ehrlichiosis caused by E. canis are similar to those caused by ...

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Molecular diagnosis of Usher syndrome: application of two different next generation sequencing-based procedures.

Molecular diagnosis of Usher syndrome: application of two different next generation sequencing-based procedures.

... The first evidence we gathered is that when using whole exome sequencing the percentage of Usher genes represented ranged from a maximum of 98% to a minimum of 76% at low sequence coverage (Figure 2B and Figure S2). ...

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Rev. Bras. Hematol. Hemoter.  vol.35 número4

Rev. Bras. Hematol. Hemoter. vol.35 número4

... A molecular study revealed biallelic mutations in the Shwachman-Bodian-Diamond Syndrome gene (183-184TA-CT K62X in exon 2 and a 258+2T-C transition) conirming the diagnosis of Shwachman-Diamond ...with ...

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Gavaa, Cristina da Silva Meira-Strejevitcha, Jose Ernesto Vidalb,c,d, Cinara Cássia Brandão de Mattose, Fábio Batista Fredericof, Luiz Carlos De Mattose, Lígia Cosentino Junqueira Franco Spegiorine,g, FAMERP Toxoplasma Research Group, Fernando Henrique An

Gavaa, Cristina da Silva Meira-Strejevitcha, Jose Ernesto Vidalb,c,d, Cinara Cássia Brandão de Mattose, Fábio Batista Fredericof, Luiz Carlos De Mattose, Lígia Cosentino Junqueira Franco Spegiorine,g, FAMERP Toxoplasma Research Group, Fernando Henrique An

... the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii ...

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Isolation and genetic characterization of Toxoplasma gondii from free-ranging and captive birds and mammals in Pernambuco state, Brazil

Isolation and genetic characterization of Toxoplasma gondii from free-ranging and captive birds and mammals in Pernambuco state, Brazil

... direct molecular diagnosis based on primary samples, Dubey (2010) reported that this technique was very effective for the diagnosis, but not so much for genotyping, due to the small amount of genetic ...

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Arq. Bras. Oftalmol.  vol.80 número5

Arq. Bras. Oftalmol. vol.80 número5

... Clinical and molecular diagnosis of a Costa Rican family with autosomal recessive myo tonia congenita (Becker disease) carrying a new mutation in the CLCN1 gene. Rev Biol Trop. Tozlova[r] ...

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Cad. Saúde Pública  vol.25 número10

Cad. Saúde Pública vol.25 número10

... tion, molecular tests, and drafting of the ...the molecular diagnosis strategy, analysis, interpretation of the results, and revision of the manuscript (molecular diagnosis) before and ...

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Detection of natural occurrence of Tritrichomonas foetus in cats in Araçatuba, São Paulo, Brazil

Detection of natural occurrence of Tritrichomonas foetus in cats in Araçatuba, São Paulo, Brazil

... the diagnosis method is the second point that may interfere with the ...foetus diagnosis than the PCR, as highlighted in previous studies (Gookin et ...2011). Molecular diagnosis is a good ...

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Genetic diversity and molecular survey of Trypanosoma (Megatrypanum) theileri in cattle in Brazil’s western Amazon region

Genetic diversity and molecular survey of Trypanosoma (Megatrypanum) theileri in cattle in Brazil’s western Amazon region

... Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that infects ruminants and it displays high genetic diversity. In this study, we investigated the prevalence rates of this protozoan based on hemoculture and ...

5

Arq. NeuroPsiquiatr.  vol.75 número9

Arq. NeuroPsiquiatr. vol.75 número9

... early diagnosis of dengue might not correlate with the early diagnosis of neuro- logical ...the diagnosis of dengue in an endemic area usually relies on the clinical diagnosis, and the role of ...

1

Molecular techniques for the study and diagnosis of parasite infection

Molecular techniques for the study and diagnosis of parasite infection

... The AFLP technology is also a technique that allows detection of a DNA polymorphism without prior information on the sequence. This technique is highly efficient because of the possibility of analyzing a large number of ...

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J. Bras. Patol. Med. Lab.  vol.44 número1

J. Bras. Patol. Med. Lab. vol.44 número1

... the molecular target found in most cases of acute promyelocytic leukemia (APL) and has been used for diagnosis and minimal residual disease ...standard molecular technique employed is qualitative ...

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J. Bras. Patol. Med. Lab.  vol.51 número1

J. Bras. Patol. Med. Lab. vol.51 número1

... the diagnosis of iron deiciency anemia and thalassemia minor, both alpha and beta, is based only on hematological parameters and erythrocyte morphology, these forms of thalassemia may be erroneously diagnosed as ...

6

Rev. Bras. Hematol. Hemoter.  vol.32 número6

Rev. Bras. Hematol. Hemoter. vol.32 número6

... the diagnosis of acute leukemia (WHO-2008) which include the cell origin and lineage, the maturation stage and type of cytogenetic or molecular abnormalities involved in the pathogenesis of the ...

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Luz Maira Wintaco Martínez, Gloria Puerto Castro

Luz Maira Wintaco Martínez, Gloria Puerto Castro

... a molecular tool for rapid detection of MDR TB and extensively drug- resistant TB based on the mutations in the rpoB, katG, inhA, ahpC, and gyrA genes by reverse hybridisation of ...

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Diagnostic methods for identification of root-knot nematodes species from Brazil

Diagnostic methods for identification of root-knot nematodes species from Brazil

... general, molecular approaches are accurate, rapid and do not require only females for diagnosis purposes, such as isozyme phenotypes and perineal ...that molecular techniques will soon be used more ...

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