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N-terminal amino acid sequence

Produção da enzima antileugêmica L-Asparaginase II a partir da clonagem do Gene ErA de Erwinia Carotovora Supsp. Atroseptica em Escherichia Coli

Produção da enzima antileugêmica L-Asparaginase II a partir da clonagem do Gene ErA de Erwinia Carotovora Supsp. Atroseptica em Escherichia Coli

... The N-terminal amino acid sequencing showed the removal of the N-terminal signal peptide from ...peptide sequence (AspMP). Amino acid sequencing ...

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Molecular cloning and characterization of novel Morus alba germin-like protein gene which encodes for a silkworm gut digestion-resistant antimicrobial protein.

Molecular cloning and characterization of novel Morus alba germin-like protein gene which encodes for a silkworm gut digestion-resistant antimicrobial protein.

... The N-terminal amino acid sequence of the purified protein extracted from silkworm fecal matter was found exactly similar to the deduced amino acid sequence ...

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Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization

Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization

... Crevalle jack pyloric caeca trypsin (91.2 U/mg of specific activity and 27.5 kDa) was easily purified approximately 102-fold, recovering about 20% of the enzyme contained in the crude extract. The use of specific ...

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N-Terminal Amino Acid Sequences of the Major Outer Membrane Proteins from a Neisseria meningitidis Group B Strain Isolated in Brazil

N-Terminal Amino Acid Sequences of the Major Outer Membrane Proteins from a Neisseria meningitidis Group B Strain Isolated in Brazil

... strains. Although its cellular function is unknown, it shares sequence homology with Escherichia coli Omp A (Klugman et al. 1989). Antibodies directed against this protein are non-bactericidal and have the ...

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Identification and characterization of the regions involved in the nuclear translocation of the heterodimeric leishmanial DNA topoisomerase IB.

Identification and characterization of the regions involved in the nuclear translocation of the heterodimeric leishmanial DNA topoisomerase IB.

... basic sequence of 43 amino acids in the C-terminal extension of the large ...10-amino acid motif located in the N-terminal extension of the protein; NLS3 consists of a ...

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Evolutionary descent of prion genes from the ZIP family of metal ion transporters.

Evolutionary descent of prion genes from the ZIP family of metal ion transporters.

... sequences N-terminal to the PL domain, could have originated from a ZIP ancestor ...respective N-terminal ...sequences N-terminal to both PrP and ZIP proteins contain similar ...

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A Novel Cytosolic Isoform of Mitochondrial Trans-2-Enoyl-CoA Reductase Enhances Peroxisome Proliferator-Activated Receptor α Activity

A Novel Cytosolic Isoform of Mitochondrial Trans-2-Enoyl-CoA Reductase Enhances Peroxisome Proliferator-Activated Receptor α Activity

... its amino acid sequence is exactly the same as that of MECR, except for the N-terminal 76 amino acids that compose the mitochondrial targeting ...

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cDNA cloning and 1.75 Å crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds

cDNA cloning and 1.75 Å crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds

... full-length amino acid sequence of Parkia platycephala lectin 2, determined by N-terminal sequencing and cDNA clo- ning, and its three-dimensional structure, established by X-ray ...

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The N-terminal 33 amino acid domain of Siva-1 is sufficient for nuclear localization

The N-terminal 33 amino acid domain of Siva-1 is sufficient for nuclear localization

... the N-terminal, SAH and DDHR ...the N- terminal region of Siva-1 is indispensable for this protein nuclear localization, which is required for attenuation of NF-kB ...of ...

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Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper

Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper

... partial amino acid sequence shows high identity with snake venom serine proteinases and a complete identity with a cDNA clone previously sequenced from this ...The N-terminal ...

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Chromosomal rearrangements and protein globularity changes in Mycobacterium tuberculosis isolates from cerebrospinal fluid

Chromosomal rearrangements and protein globularity changes in Mycobacterium tuberculosis isolates from cerebrospinal fluid

... The amino acid sequence of a protein determines its folding into three-dimensional structures which may be ordered (globular with high hydrophobicity) or disordered (unstructured, typically with low ...

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Comparative genomics of an endophytic Pseudomonas putida isolated from

Comparative genomics of an endophytic Pseudomonas putida isolated from

... Isolation of Pseudomonas putida TJI51 has been de- scribed elsewhere (Khan et al., 2014). Briefly, Pseudomo- nas putida TJI51 was isolated from bark of a mango tree situated in the Horticultural Garden, Sindh Agriculture ...

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Cloning, structural modelling and characterization of VesT2s, a wasp venom hyaluronidase (HAase) from Vespa tropica

Cloning, structural modelling and characterization of VesT2s, a wasp venom hyaluronidase (HAase) from Vespa tropica

... The basic characterizations of the gene and protein se- quences were analyzed using NCBI (http://www.ncbi.nlm. nih.gov/Database/index.html) and the basic local alignment search tool BLAST ...

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Proteolytic Processing of the Cyt1Ab1 Toxin Produced by Bacillus thuringiensis subsp. medellin

Proteolytic Processing of the Cyt1Ab1 Toxin Produced by Bacillus thuringiensis subsp. medellin

... activity. Amino terminal sequence of the ...a sequence DDPNEKNNHNS; while for the trypsin-resistant fragment the cutting site was determined between Leu 29 and Arg 30 , and for the ...

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Molecular evidence for a functional ecdysone signaling system in Brugia malayi.

Molecular evidence for a functional ecdysone signaling system in Brugia malayi.

... high sequence similarity to Di-RXR-1 from ...Procedures; sequence deposited in GenBank ...the sequence information from the other filarial species as well as the Brugia malayi genome project we ...

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Computational Identification of Protein Pupylation Sites by Using Profile-Based Composition of k-Spaced Amino Acid Pairs.

Computational Identification of Protein Pupylation Sites by Using Profile-Based Composition of k-Spaced Amino Acid Pairs.

... k-spaced amino acid pair. As a sketch of sequence motif, k-spaced amino acid pair could better reflect the coor- dinated pairs of residues surrounding the pupylation ...Table, ...

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MATERIALS AND METHODS Dog and human blood specimens

MATERIALS AND METHODS Dog and human blood specimens

... rRNA sequence of strain Lara was different from another Venezuela strain from Maracaibo, suggesting that there are at least two strains of ...groESL sequence was found to be identical to the sequence ...

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A Conserved LIR Motif in Connexins Mediates Ubiquitin-Independent Binding to LC3/GABARAP Proteins

A Conserved LIR Motif in Connexins Mediates Ubiquitin-Independent Binding to LC3/GABARAP Proteins

... the amino acid sequence of rat Cx43 through the iLIR webtool [18] revealed a potential LIR motif in the amino terminal of the protein with core residues corresponding to tryptophan 4 ...

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Conserved domains and evolution of secreted phospholipases A2

Conserved domains and evolution of secreted phospholipases A2

... consensus sequence, the expected-value for the align- ment, the identity (name) of the conserved domain and the location of the conserved domain in the query sequence ...aspartic acid active-site ...

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PredPPCrys: accurate prediction of sequence cloning, protein production, purification and crystallization propensity from protein sequences using multi-step heterogeneous feature fusion and selection.

PredPPCrys: accurate prediction of sequence cloning, protein production, purification and crystallization propensity from protein sequences using multi-step heterogeneous feature fusion and selection.

... use sequence-derived features that can be readily exploited by experimental ...with sequence lengths ranging from 46 to 200 amino ...of sequence-derived features to train their models and ...

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