Nucleic Acid Amplification
Digital microfluidics for nucleic acid amplification
... Nucleic acid amplification is one of the most relevant tools in molecular biology, allowing the monitorization of gene expression, quantification of food-borne pathogens, diagnostic of hereditary and ...
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Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
... (Cartwright & Kroll 1997). Preadmission parenteral an- tibiotic is currently recommended and less lumbar punctures are done in patients with suspected meningococcal disease at presentation (Carrol et al. 2000). ...
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Chlamydia detection during the menstrual cycle: a cross-sectional study of women attending a sexual health service.
... by nucleic acid amplification of high vaginal, cervical, or urinary samples, and who recorded a date of last normal menstrual period (LNMP) between 0–28 days were included in the ...
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J. Bras. Nefrol. vol.38 número3
... quantitative nucleic acid amplification testing (NAT) by polymerase chain reaction (PCR) to identify BKPyV in urine or plasma or blood samples, or urine electronic microscopy to detect ...
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Análise comparativa de métodos de diagnóstico para linfadenite caseosa em ovinos sintomáticos e assintomáticos
... confirmed? Extension Veterinarian, UC Davis. Nucleic acid amplification of Mycobacterium tuberculosis complex DNA from archival fine needle aspiration smear scrapings vs. fresh fine[r] ...
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Molecular Staging of Patients with Colon Cancer. The C-Closer-II Study: A Multicentre Study in Portugal
... step nucleic acid amplification) assay (Sysmex Corp., Japan) uses the RT-LAMP (reverse transcription loop- mediated isothermal amplification) technique to rapidly amplify the CK19 mRNA tumour ...
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Rev. Bras. Hematol. Hemoter. vol.30 número4
... 6. Scuracchio PS, Poli MC, Lemos MM, Oliveira AG, Salles NA, Chamone DA et al. Detection of HIV-1 infection in blood donors during the immunological window period using the nucleic acid- ...
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Ecophysiological traits of highly mobile large marine predators inferred from nucleic acid derived indices
... of the biopsied cetaceans were chemically (cold sarcosyl Tris-EDTA extraction buffer) and mechanically (through sonification – 3 pulses 50 A for 1 min, and vortex) homogenised, and after centrifugation the tissues were ...
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Affinity chromatography in plasmid DNA purification for therapeutic applications
... and nucleic acid bases [6,7], it was recently developed by our research group a new affinity chro- matographic methodology using some amino acids immobilized on agarose matrix for the isolation and ...
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Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil
... for amplification and detection of hMPV and hBoV and to evaluate coinfections with respi- ratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human ...
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M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A.
... Bone-marrow was isolated by flushing the tibia and the femur of WT, MyD88 2/2 and TRIF 2/2 mice (all in the Bl/6 background) with ice-cold PBS. After lysing red blood cells with ACK red blood cell lysis buffer ...
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Rev. Bras. Hematol. Hemoter. vol.35 número4
... 8. Levi JE, Pereira RA, Polite MB, Mota MA, Nunez SP, Pinho JR, et al. One window-period donation in two years of individual donor-nucleic acid test screening for hepatitis B, hepatitis C and human ...
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PEGylated polyethyleneimine-entrapped gold nanoparticles for enhanced and targeted gene delivery applications
... non-viral nucleic acid vector system for enhanced and targeted nucleic acid delivery applications was ...folic acid (FA; for targeted delivery to cancer cells overexpressing FA ...
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Chitosan and its derivatives as nanocarriers for siRNA delivery
... Recently, a new multifunctional magnetic nanovector was developed for dual imaging and therapeutic purposes. It is comprised of a superparamagnetic iron oxide nanoparticle core, coated with polyethyleneglycol-grafted ...
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Identification of novel viruses using VirusHunter--an automated data analysis pipeline.
... Quick and accurate identification of microbial pathogens is essential for both diagnosis and response to emerging infectious diseases. High-throughput sequencing has recently emerged as a powerful approach to identify ...
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Heterotrophic prokaryote distribution along a 2300 km transect in the North Pacific subtropical gyre during a strong La Niña conditions: relationship between distribution and hydrological conditions
... To analyse the multivariate data set, principal component analyses (PCA) and redundancy analysis (RDA) were per- formed using the R software (vegan package) and the Biplot macro for Excel ® (Lipkovich and Smith, 2002). ...
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From the field to the lab: best practices for field preservation of bat specimens for molecular analyses.
... spectrophotometric nucleic acid quantification and quantitative PCR (qPCR) of a single-copy nuclear gene, was conducted between sample types (wing punch ...
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From SOMAmer-based biomarker discovery to diagnostic and clinical applications: a SOMAmer-based, streamlined multiplex proteomic assay.
... bead-based nucleic acid (SOMAmer) readout, is sufficiently sensitive and robust to separate case and control populations within a clinical sample ...
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pH-dependent conformational changes in the HCV NS3 protein modulate its ATPase and helicase activities.
... Lam and colleagues [25] have proposed model mechanism for NS3hel translocation along RNA that would be activated by acidic pH. In this model, NS3hel exists in two basic conformations, and the transition between the two ...
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Rev. Bras. Hematol. Hemoter. vol.35 número3
... by nucleic acid testing, 31 were also serologically reactive and one irst-time donor was identiied as having hepatitis B in the window ...were nucleic acid testing non-reactive and no human ...
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