PCR to detection the M. leprae DNA
Detection of Lsr2 Gene of Mycobacterium leprae in Nasal Mucus
... responses to specific sequences within the Lsr antigen have been shown to be associated with the immunopathological responses in leprosy patients with erythema nodosum ...localized to ...
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Detecção e quantificação de mycobacterium leprae pela técnica de pcr em tempo real em amostras ambientais de municípios do Ceará
... Mycobacterium leprae, an intracellular agent unable to grown in axenic culture ...overall detection ratio of 24.0 / 100,000 inhabitants. Transmission is related to the bacilli eliminate ...
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Deteccção do DNA de Mycobacterium leprae em secreção nasal.
... of the polimerase chain reaction (PCR) technique in the identification of the Mycobacterium leprae DNA ...aimed to evalue the PCR sensibility at the ...
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J. bras. pneumol. vol.33 número4 en v33n4a13
... min. The DNA was extracted using QIAamp Tissue DNA Kit (Qiagen, Hilden, Germany), according to the manufactur- er’s ...Purified DNA (10 µl) was added to 40 µl of reaction ...
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Ehrlichia canis and Rickettsia spp. in dogs from urban areas in Paraiba state, northeastern Brazil
... Parola P, Paddock CD, Socolovschi C, Labruna MB, Mediannikov O, Kernif T, et al. Update on Tick-Borne Rickettsioses around the World: a Geographic Approach. Clin Microbiol Rev 2013; 26(4): 657-702. PMid:24092850. ...
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In vitro culture, PCR , and nested PCR for the detection of Theileria equi in horses submitted to exercise
... tests to identify infected animals whose movement should be restricted, several other techniques including in vitro cultures, DNA probes, and polymerase chain reaction (PCR) have been used for ...
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Selection of the temperature of casting the bronzes to plaster moulds
... of the drop of the solidification bronze on plaster mould and the fragments of plaster mould from the side of the contact with the melted drop show on Figure ...station to ...
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Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
... for the introduction and dissemination of stalk and ear rot and macrospore leaf spot ...diseases. the use of healthy seeds is an important strategy for the preventive control of these ...of ...
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Perfil de marcadores sorológicos, salivares e moleculares de Mycobacterium leprae entre contatos de pacientes com hanseníase
... de DNA de Mycobacterium leprae , através da Reação em Cadeia Polimerase (PCR), na mucosa nasal e anticorpos anti-PGL1 séricos, IgG/IgM, e salivares, IgA/IgM, por meio do ensaio imunoenzimático ligado ...
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ASSESSMENT OF PCR IN THE DETECTION OF
... by the polymerase chain reaction (PCR) was applied in the investigation of the presence of Leishmania (Kinetoplastida: Trypanosomatidae) parasites in single phlebotomine ...in the ...
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Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction.
... are the lead causes of bacterial meningitis. Detection of these pathogens from clinical specimens using traditional real-time PCR (rt-PCR) requires DNA extraction to remove ...
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Repositório Institucional UFC: Associação do câncer de mama com a infecção pelo papilomavírus humano (HPV): evidência molecular
... do DNA do HPV em cânceres de ...nova PCR, do tipo nested multiplex, para região de consenso E6/E7 do HPV com o objetivo de identificar tipos ...O DNA do HPV foi detectado em 49,5% dos carcinomas ...
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DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL
... were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental ...resemblance to V. cholerae were ...
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Arq. NeuroPsiquiatr. vol.55 número4
... Herpes simplex virus (HSV) DNA detection in cerebrospinal fluid (CSF) samples by polymerase chain reaction (PCR) has been recently evaluated as a rapid non-invasive diagnostic assay for[r] ...
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A Method To Find The Area Of Sector Without The Usage Of Angle Made By The Chord
... at the alternate sides, of the tangents through the diameter of the circle, and then the line joining these points divides the circle in the ratio of the distances ...
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Gold Nanoparticles for the Detection of DNA Adducts as Biomarkers of Exposure to Acrylamide
... increased DNA damage, which supports the fact that acrylamide per se has low reactivity to DNA, prior to CYP ...dose-dependent DNA damage in different tissues ...According ...
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DETECTION OF MYCOBACTERIUM BOVIS DNA IN NASAL SWABS FROM TUBERCULOUS CATTLE BY A MULTIPLEX PCR Eduardo Eustáquio de Souza Figueiredo
... study, the aim was to standardize a multiplex PCR- based method for the simultaneous detection of mycobacteria belonging to MTC and the specific identification of ...using ...
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RETROSPECTIVE STUDY ON PORCINE CIRCOVIRUS-2 BY NESTED PCR AND REAL TIME PCR IN ARCHIVED TISSUES FROM 1978 IN BRAZIL Fernanda Miquelitto Figueira da Silva
... PCV-2 DNA quantification assays. The plasmid was purified using the commercial kit Wizard Plus SV Minipreps DNA Purification System (PROMEGA, Madison, USA), and quantified by ...
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Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme.
... sensitivity to three leading commercial two-step RT-PCR kits when used to detect either MS2 phage or influenza A ...in the absence of target, especially after 40 cycles of PCR, is ...
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Detecção molecular de Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) em procionídeos (Carnivora: Procyonidae) na Amazônia Oriental, Brasil Paulo Cesar Magalhães-MatosI Rafaelle Cunha-SantosI Paulo Geovani Silva SousaI Francisco Dantas Sampaio-Júnio
... on the natural infection of procyonids by ...about the occurrence of this pathogen in captive animals. In the present study, a significant association was observed between captive animals and ...
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