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Polymerase Chain Reaction

Diagnosis of Dengue by Using Reverse Transcriptase- Polymerase Chain Reaction

Diagnosis of Dengue by Using Reverse Transcriptase- Polymerase Chain Reaction

... Characterization of DEN-1 and DEN-2 viruses by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Ethidium bro- mide stained agarose gel showing RT-PCR products: lane 1, molecular weight ...

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Aseptic meningitis caused by Leptospira spp diagnosed by polymerase chain reaction

Aseptic meningitis caused by Leptospira spp diagnosed by polymerase chain reaction

... The polymerase chain reaction (PCR) is becoming a common diagnostic tool for the early and accurate diagnosis of bacterial meningitis (Ni et ...

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Molecular Identification of Similar Species of the Genus Biomphalaria (Mollusca: Planorbidae) Determined by a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

Molecular Identification of Similar Species of the Genus Biomphalaria (Mollusca: Planorbidae) Determined by a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

... The freshwater snails Biomphalaria straminea, B. intermedia, B. kuhniana and B. peregrina, are morphologically similar; based on this similarity the first three species were therefore grouped in the complex B. straminea. ...

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NEW PRIMERS FOR DETECTION OF Leishmania infantum USING POLYMERASE CHAIN REACTION

NEW PRIMERS FOR DETECTION OF Leishmania infantum USING POLYMERASE CHAIN REACTION

... Polymerase Chain Reaction: For PCR standardization, some conditions were ...The reaction mixture contained ...DNA polymerase (Invitrogen Life Technologies, Sao Paulo, Brazil), ...

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Parasitology Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis Diagnosis of cutaneous leishmaniasis by polymerase chain reaction

Parasitology Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis Diagnosis of cutaneous leishmaniasis by polymerase chain reaction

... of polymerase chain reaction (PCR) was similar to that of immunofluorescence assay and enzyme-linked immunosorbent assay, and higher than that of MST and PE ( P b ...

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Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)

... the polymerase chain reaction (PCR) technique, isolated from small intestine samples of bovines that have died suddenly, after manifesting or not enteric or neurological ...

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Brazilian ground beef authentication by multiplex polymerase chain reaction

Brazilian ground beef authentication by multiplex polymerase chain reaction

... tissues. Polymerase chain reaction (PCR) is an efficient method for detecting adulteration, and this method has already been usedto detect adulteration in meat products (DAGUER et ...

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A diagnosis of Burkholderia pseudomallei directly in a  lavage by polymerase chain reaction

A diagnosis of Burkholderia pseudomallei directly in a lavage by polymerase chain reaction

... Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B ...

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A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia

A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia

... Dark P, Dunn G, Chadwick P, Young D, Bentley A, Carlson G, War- hurst G 2011. The clinical diagnostic accuracy of rapid detection of healthcare-associated bloodstream infection in intensive care using multi-pathogen ...

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Detecção do complexo Mycobacterium tuberculosis por nested polymerase chain reaction em espécimes pulmonares e extrapulmonares.

Detecção do complexo Mycobacterium tuberculosis por nested polymerase chain reaction em espécimes pulmonares e extrapulmonares.

... nested polymerase chain reaction (NPCR) com aquele de culturas na detecção do complexo Mycobacterium tuberculosis em espécimes pulmonares e ...

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Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis

Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis

... of polymerase chain reaction (PCR) in the diagnosis of genital campylobacteriosis in samples obtained from bull prepuce aspirate, cow cervical mucus, and abomasum contents of aborted fetuses, ...

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Modelling of Serpentine Continuous Flow Polymerase Chain Reaction Microfluidics

Modelling of Serpentine Continuous Flow Polymerase Chain Reaction Microfluidics

... flow Polymerase Chain Reaction (PCR) microfluidics DNA amplification device is a recent discovery aimed at eliminating the cyclic hold experienced while using the alternative stationary ...

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Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)

... transcription-quantitative polymerase chain reaction (RT-qPCR) RNA was extracted using the UNIQ-10 column TRIzol kit (Shanghai Sangon Biotech, Shanghai, China) and treated with ...PCR reaction ...

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DIAGNOSIS OF THE PULMONARY TUBERCULOSIS BY POLYMERASE CHAIN REACTION: A COMPARATIVE STUDY BETWEEN HIV-POSITIVE AND -NEGATIVE INDIVIDUALS Rosemeri Maurici da Silva

DIAGNOSIS OF THE PULMONARY TUBERCULOSIS BY POLYMERASE CHAIN REACTION: A COMPARATIVE STUDY BETWEEN HIV-POSITIVE AND -NEGATIVE INDIVIDUALS Rosemeri Maurici da Silva

... of polymerase chain reaction (PCR) directly from sputum for the diagnosis of pulmonary tuberculosis by comparison between HIV-positive and HIV-negative ...

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Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase

Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase

... the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of ...DNA polymerase, an enzyme that would survive the high temperatures needed for DNA ...Taq ...

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Polymerase chain reaction – PCR

Polymerase chain reaction – PCR

... C. perfringens produces a variety of exotoxins; four of these - alpha, beta, epsilon, and iota - are considered the major toxins and are used to group the bacteria into five types A, B, C, D, and E. Alpha toxin is ...

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Detection of human papillomaviruses by polymerase chain reaction and ligation reaction on universal microarray.

Detection of human papillomaviruses by polymerase chain reaction and ligation reaction on universal microarray.

... Here we report a highly sensitive and specific DNA microarray method for genotyping of 15 different hrHPVs and two low-risk HPVs (lrHPVs). The method is based on multiplex PCR amplification of the viral L1 region using ...

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Detection of genomic mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis isolates using polymerase chain reaction and multiplex allele-specific polymerase chain reaction

Detection of genomic mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis isolates using polymerase chain reaction and multiplex allele-specific polymerase chain reaction

... The amount of each pair of primers in MAS-PCR was balanced to achieve acceptable amplification of all target regions. For each MAS-PCR reaction, a standard 35 µL final reaction was used. Each final volume ...

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Polymerase chain reaction (PCR)

Polymerase chain reaction (PCR)

... The amplification was carried out in a PC Thermocycler (Biometra, Germany), using an initial heating of 95°C for 5 min. Each cycle was divided into three stages: denatur- ation (95°C – 1.5 min), annealing, and elongation ...

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Polymerase Chain Reaction (PCR) Identification of Terverticillate

Polymerase Chain Reaction (PCR) Identification of Terverticillate

... (Fermentas), 2.5 µL 25 mM MgCl (Fermentas), 2 µl 2.5 mM dNTPmix, 0.25 µL 5 U/ µL Taq DNA polymerase (Fermentas), and 11.75 µL sterile deionized water. DNA amplification was performed in a thermocycler with an ...

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