Polymorphic Dna Microsatellite Locus
Development of 22 Polymorphic Microsatellite Loci for the Critically Endangered Morato's Digger Toad, Proceratophrys moratoi
... the locus-specific annealing temperature (Table 1), and 1 min at 72 °C, followed by a final extension of 30 min at 72 °C to minimize stutter ...bp DNA ladder (Invitrogen) and using the GelAnalyzer 2010a ...
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Development and Integration of Genome-Wide Polymorphic Microsatellite Markers onto a Reference Linkage Map for Constructing a High-Density Genetic Map of Chickpea.
... long DNA motifs present in coding and non-coding sequence components of crop genomes ...of microsatellite repeats at a locus among indi- viduals results in different sized amplicons, thereby making ...
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Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
... or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet ...orange microsatellite loci revealed that AG/TC was the most abundant (69%) ...
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Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazama gouazoubira
... observed locus-specific allele size ranges and screened for polymorphisms using template DNA from 15 individu- als captured in the wild, in the central region of the Brazil- ian Pantanal (state of Mato ...
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Characterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa burmeisteri and cross-species amplification
... We estimated polymorphism levels by genotyping a total of 25 individuals from 2 populations of the Minas Gerais State: Juiz de Fora (N = 12; S 21.7325°, W 43.3696°) and Carangola (N = 13; S 20.7986°, W 42.0836°) using 2X ...
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Benefits of Turbid River Plume Habitat for Lake Erie Yellow Perch (Perca flavescens) Recruitment Determined by Juvenile to Larval Genotype Assignment.
... use microsatellite markers and genotype assignment between life stages (juvenile to lar- val) to test the hypothesis that survival of larval yellow perch (Perca flavescens; YP, hereafter) to the age-0 juvenile ...
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5meCpG epigenetic marks neighboring a primate-conserved core promoter short tandem repeat indicate X-chromosome inactivation.
... (XY). DNA methylation at the carbon-5 position of the cytosine pyrimidine ring in the context of a CpG dinucleotide sequence (5 me CpG) in promoter regions is a key epigenetic marker for transcriptional gene ...
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Polymorphic DNA microsatellite markers for forensic individual identification and parentage analyses of seven threatened species of parrots (family Psittacidae)
... wildlife DNA forensics to determine with conclusive evidence illegal trade, current usage of DNA profiling approaches in parrots has been limited by the lack of suitable molecular markers specifically ...
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Molecular techniques for the study and diagnosis of parasite infection
... enables DNA amplification with high specificity, sensitivity, and rapidity under isothermal ...conditions. DNA amplification can be achieved using simple incubators (water bath or block heater) because of ...
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Caracterização da diversidade genética em feijão por meio de marcadores RAPD.
... A aplicabilidade de marcadores RAPD em estabelecer a relação genética de germoplasmas de feijão, mesmo entre os estreitamente relacionadas (mesmo pool gênico) é mais uma vez comprovada neste estudo. Os resultados obti- ...
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ISSN 1516-635X Jul - Sept 2016 v.18 n.3 519-524 http:dx.doi.org10.15901806-9061-2015-0101 Microsatellite Analysis of Genetic Diversity in Quail Populations from China
... 12 microsatellite markers in the three quail communities are shown in Table ...12 microsatellite markers, UBC0004 presented the least effective alleles ...
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Determination of microsatellite profiles Initial DNA preparation for elaboration of a sub-library - Total DNA
... ABSTRACT - In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved ...
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Guilherme da Silva Pereira (1) , Ana Luíza Ramos Cazé
... identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the ...were polymorphic, two of which were amplified by one ...
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Differentiation and Genetic Analysis of Rhodnius prolixus and Rhodnius colombiensis by rDNA and RAPD Amplification
... amplified polymorphic DNA amplification patterns and cluster analysis suggests reproductive discontinuity between the two spe- ...ribosomal DNA supports these ...
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Key words: MLVA, VTEC O157:H7, molecular characterization, typing
... Short motif VNTRs with repeat units harboring less than six bases are prone to variation in unit number at a given locus (25), and most of the VNTR loci included in this MLVA protocol are short motif repeats with ...
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Riva P Oliveira, Anna Izabel R Melo, Andréa M Macedo, Egler Chiari, Sérgio DJ Pena
... Polymorphism of CA-repeats in T. cruzi - We first performed an analysis of seven different clones with the eight CA-repeat microsatellite loci. All these clones showed amplification of only one or two fragments of ...
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J. Bras. Patol. Med. Lab. vol.51 número3
... fundamental principles of these molecular diagnostic techniques is important for their implementation in routine clinical practice. However, individual expertise, institutional and inancial issues should be taken into ...
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Morphological and molecular diversity among cassava genotypes
... Abstract – The objective of this work was to characterize morphologically and molecularly the genetic diversity of cassava accessions, collected from different regions in Brazil. A descriptive analysis was made for 12 ...
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Genome-wide identification of regulatory elements and reconstruction of gene regulatory networks of the green alga Chlamydomonas reinhardtii under carbon deprivation.
... reinhardtii, DNA fragments present in the FAIRE samples were sequenced by deep sequencing ...the DNA sample was fractionated on a 2% agarose gel and fragments between 200 to 400 bp were used for the ...
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Genetic variability and homozygosity in a F4 castor bean population by microsatellite markers
... for DNA extraction according to the protocol described by Doyle and Doyle ...of DNA were evaluated by comparative analysis with known concentrations of lambda DNA (Invitrogen, Carlsbad, CA, USA) in ...
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