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protease activity

The threonine protease activity of testes-specific protease 50 (TSP50) is essential for its function in cell proliferation.

The threonine protease activity of testes-specific protease 50 (TSP50) is essential for its function in cell proliferation.

... Upon reaching confluency, CHO cells showed no contact- induced growth arrest, but they did undergo high-density dependent apoptosis [16]. Figure 3A shows that, until day 4 there was no significant difference in the ...

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Caveolin-1 influences vascular protease activity and is a potential stabilizing factor in human atherosclerotic disease.

Caveolin-1 influences vascular protease activity and is a potential stabilizing factor in human atherosclerotic disease.

... MMP-9 activity plays a major role in expansive remodeling in animal models ...and protease activity that remains clinically silent and untreated for a long ...

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Protease activity in Giardia duodenalis trophozoites of axenic strains isolated from symptomatic and asymptomatic patients

Protease activity in Giardia duodenalis trophozoites of axenic strains isolated from symptomatic and asymptomatic patients

... - Protease activities in trophozoite lysates of ...of protease activity in the BTU-10 lysate showed five bands: a group of three bands with molecu- lar masses of 92, 86 and 74 kDa (bands 1-3) and two ...

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Nepenthesin protease activity indicates digestive fluid dynamics in carnivorous nepenthes plants.

Nepenthesin protease activity indicates digestive fluid dynamics in carnivorous nepenthes plants.

... Measuring protease activity with fluorescent substrate and FRET Using a small and highly specific FRET peptide substrate (FITC(Ahx)-Val-Val-LysDbc), en- coded as PFU-093 by Kaman et ...proteolytic ...

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Nitrated Fatty Acids Reverse Cigarette Smoke-Induced Alveolar Macrophage Activation and Inhibit Protease Activity via Electrophilic S-Alkylation.

Nitrated Fatty Acids Reverse Cigarette Smoke-Induced Alveolar Macrophage Activation and Inhibit Protease Activity via Electrophilic S-Alkylation.

... Among the many cell types that contribute to COPD pathophysiology, alveolar macro- phages (AMs) are especially critical [3]. They are directly exposed to cigarette smoke, the major risk factor for COPD, and other noxious ...

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Assay of Protease Activity and Protein Content

Assay of Protease Activity and Protein Content

... Salt precipitation and chromatography have commonly been employed for the purification of enzymes (Bajaj and Singh 2010; Mahajan et al. 2012). B. subtilis I-2 protease was purified by 4.8- fold with a recovery of ...

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Partial characterization of digestive proteases in sheepshead, Archosargus probatocephalus (Spariformes: Sparidae)

Partial characterization of digestive proteases in sheepshead, Archosargus probatocephalus (Spariformes: Sparidae)

... enzymatic activity present in the digestive system, particularly in the case of proteases, since protein is the most important nutrient in re- lation to energy and amino acid requirements in fish (Lazo et ...the ...

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MATERIALS AND METHODS Organism

MATERIALS AND METHODS Organism

... halophilic protease was purified using ultrafiltration, ethanol precipitation, hydrophobic interaction column chromatography and gel permeation chromatography to 180 fold with 22% ...the protease determined ...

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OPTIMIZATION OF GROWTH MEDIUM FOR PROTEASE PRODUCTION BY HALOFERAX LUCENTENSIS VKMM 007 BY RESPONSE SURFACE METHODOLOGY Muthu Manikandan

OPTIMIZATION OF GROWTH MEDIUM FOR PROTEASE PRODUCTION BY HALOFERAX LUCENTENSIS VKMM 007 BY RESPONSE SURFACE METHODOLOGY Muthu Manikandan

... of protease activity was carried out according to McDonald and Chen ...of protease activity was expressed as the amount of enzyme that liberated 1 µM tyrosine min -1 under assay ...

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FRET peptides reveal differential proteolytic activation in intraerythrocytic stages of the malaria parasites Plasmodium berghei and Plasmodium yoelii

FRET peptides reveal differential proteolytic activation in intraerythrocytic stages of the malaria parasites Plasmodium berghei and Plasmodium yoelii

... The effects of Ca 2+ modulation on protease activity of P. falcipa- rum and P. chabaudi have been investigated by Farias and colleagues (2005), who studied the proteolysis of fluorescence res- onance energy ...

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Profiling of substrate specificity of SARS-CoV 3CL.

Profiling of substrate specificity of SARS-CoV 3CL.

... Goetz et al. used a fully degenerate tetrapeptide library to study the substrate specificity of 3CL pro at P4 to P1 positions [19]. The library consisted of 20|4 sub-libraries, each consisted of a mixture of 20 3 ...

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Enzymatic activity of proteases and its isoenzymes in fermentation process in cultivars of cocoa (Theobroma cacao L.) produced in southern Bahia, Brazil

Enzymatic activity of proteases and its isoenzymes in fermentation process in cultivars of cocoa (Theobroma cacao L.) produced in southern Bahia, Brazil

... determining protease activity (under predetermined conditions) and its isoenzymes in two cocoa cultivars (PH-16 and HRT-1188) in different cocoa fermentation times, in addition to establishing the microbial ...

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BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY

... enzyme activity was defined as the amount of enzyme that liberated 1 µg of tyrosine from substrate (casein) per minute under assay conditions and reported in terms of protease activity per gram dry ...

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Effect of Feed Additives on the Development of Proteolytic Enzymes of the Tropical Sport Fish Malaysian Mahseer (Tor tambroides-Bleeker) Fry

Effect of Feed Additives on the Development of Proteolytic Enzymes of the Tropical Sport Fish Malaysian Mahseer (<i>Tor tambroides</i>-Bleeker) Fry

... tryptic activity and proteolytic action have been found in larvae fed on Artemia that of artificial diet for carp [13] ...trypsin activity in the carnivorous fish is four times higher than chymotrypsin ...

3

Inhibition of the host proteasome facilitates papaya ringspot virus accumulation and proteosomal catalytic activity is modulated by viral factor HcPro.

Inhibition of the host proteasome facilitates papaya ringspot virus accumulation and proteosomal catalytic activity is modulated by viral factor HcPro.

... To investigate the consequences of HcPro-PAA interaction on the protease activity of the proteasome, the amount of total ubiquitinated protein was estimated from the N. benthamiana leaves infiltrated with ...

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PHYSIOLOGICAL REGULATION OF PROTEASE AND ANTIBIOTICS IN PENICILLIUM SP. USING SUBMERGED AND SOLID STATE FERMENTATION TECHNIQUES

PHYSIOLOGICAL REGULATION OF PROTEASE AND ANTIBIOTICS IN PENICILLIUM SP. USING SUBMERGED AND SOLID STATE FERMENTATION TECHNIQUES

... Antibiotics, protease activity and pH values were determined after cultivation of the fungus using submerged fermentation (SF) and solid state fermentation ...

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NS3 protease from flavivirus as a target for designing antiviral inhibitors against dengue virus

NS3 protease from flavivirus as a target for designing antiviral inhibitors against dengue virus

... catalytic activity of flaviviral proteases with natural polyprotein sub- strates (Li et ...viral protease acti- vation (Falgout et ...the protease activity of NS3pro (Speroni et ...

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Protease prospecion and determination of its isoenzymes activity in cocoa cultivars (Theobroma cacao L.)

Protease prospecion and determination of its isoenzymes activity in cocoa cultivars (Theobroma cacao L.)

... The protease activity in pulp and seed was determined by taking aliquots of 100 l of the purified extract and mixing them to 100 of Tris-HCl 0.01M buffer pH 9. To this mixture, 100 l of substrate ...

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Different feeding habits influence the activity of digestive enzymes in freshwater fish

Different feeding habits influence the activity of digestive enzymes in freshwater fish

... the activity of some digestive enzymes in four fish species with different feeding ...enzymatic activity: stomach, anterior, mid and posterior ...trypsin activity was observed in all gut portions of ...

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MATERIALS AND METHODS Sampling, culturing procedures and identification of yeasts

MATERIALS AND METHODS Sampling, culturing procedures and identification of yeasts

... and protease activity were positive and had highly expressed elevations, which confirms the idea that the virulence of ...and protease secretions, leading to the belief that they must be the most ...

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