Recombinant E. coli
RESPONSE SURFACE METHODOLOGY OF NITRILASE PRODUCTION BY RECOMBINANT ESCHERICHIA COLI
... Four experimental factors; fructose, tryptone, yeast extract and lactose were chosen for the enhancement of growth and enzyme production by recombinant E. coli. They were found to have the most significant ...
8
Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity
... IPTG, recombinant E. coli BL21(DE3) was collected by cen- trifugation at 3000 g, washed twice with phosphate-buffered saline (PBS) and resus- pended in 1/50 volumes ...E. coli cultures by adding 15 ...
8
Biocatalytic Production of Trehalose from Maltose by Using Whole Cells of Permeabilized Recombinant Escherichia coli.
... Trehalose is a non-reducing disaccharide, which can protect proteins, lipid membranes, and cells from desiccation, refrigeration, dehydration, and other harsh environments. Treha- lose can be produced by different ...
11
Production of recombinant proteins in Escherichia coli
... of recombinant genes is often desirable or necessary since leaky expression can be detrimental or even lethal to cell ...of recombinant proteins due to two main reasons: First, they do not allow the ...
12
Induced Immune Response of Escherichia coli BL21 Expressing Recombinant MSP1a and MSP1b Proteins of Anaplasma marginale
... Escherichia coli BL21 expressando as proteínas recombinantes rMSP1a e rMSP1b de Anaplasma ...E. coli BL21 foi transformada com os plasmídios recombinantes pET102/msp1α e pET101/msp1β e as proteínas rMSP1a e ...
8
Genetic fusions of a CFA/I/II/IV MEFA (multiepitope fusion antigen) and a toxoid fusion of heat-stable toxin (STa) and heat-labile toxin (LT) of enterotoxigenic Escherichia coli (ETEC) retain broad anti-CFA and antitoxin antigenicity.
... E. coli recombinant strains expressing CS1 or CS2, using Caco-2 cells (ATCC, #HTB-37) as previously described ...E. coli bacteria expressing CFA/I, CFA/II or CFA/IV adhere to Caco-2 cells, whereas ...
20
Produção da proteína recombinante anexina V humana em cultivos de Escherichia coli em batelada alimentada
... the recombinant annexin V produced binds to PS and detects cells undergoing apoptosis, we produced a home-made kit la eli g rhANXA5 ith FluoroTag™ FITC Conjugation Kit (SigmaAldrich R ...
53
Análise, in vitro, da redução de endotoxinas em canais radiculares contaminados, após o emprego de sistemas de gás ozônio e eletrofulguração
... A presença da endotoxina (LPS) bacteriana no sistema de canais radiculares está relacionada com a manutenção e evolução das doenças pulpares e periapicais. Até o momento, não há nenhuma técnica ou material que seja ...
101
Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli
... The biolum inescence-based test on E. coli allows for fast determ ination of non-specific toxicity of a sam ple. In addition, another m odification of the m ethod was recently developed, whereby prolonged chronic ...
9
Recombinant Technology and Probiotics
... Risk exposure determination, risk assessment and safety assessment are essential to ensure protection for the population against any unintended consequences of the use of probiotics [25], as well as against general ...
6
Recombinant hepatitis C virus-envelope protein 2 interactions with low-density lipoproteinCD81 receptors
... Inhibiting recombinant E2 proteins from binding to cell receptors - In HUVEC, blocking LDLr led to a sig- nificant reduction in E2B and E29 binding both in the presence and absence of FCS and/or LDL in the media ...
9
Caracterização fenotípica e genotípica da resistência a antimicrobianos e análise da diversidade genética em amostras de Escherichia coli produtoras de toxina Shiga e Escherichia coli isoladas de sítios extra-intestinais
... E. coli patogênica extraintestinal (ExPEC) se possuísse ao menos 2 dos seguintes fatores de virulência: fímbria P (fímbria associada a pielonefrite codificada pelo gene papC), adesina fimbrial (sfaS), adesina da ...
170
RECOMBINANT HORSERADISH PEROXIDASE FOR ANALYTICAL APPLICATIONS
... E. coli in the form of inclusion ...heme) recombinant per - oxidase used to be rather low [9, 10], which has so far precluded the use of this enzyme in the genetic fusion ...E. coli and reactivation ...
8
Development and Characterization of Recombinant Antibody Fragments That Recognize and Neutralize In Vitro Stx2 Toxin from Shiga Toxin-Producing Escherichia coli
... The scFv antibody fragment constructed here was obtained from a bacteria-induced culture and showed a diagnostic ability, since it was able to detect the STEC strains by ELISA. None of the commercially available ...
15
Caracterização molecular de fragmentos de anticorpos anti-ssDNA obtidos a partir de uma biblioteca combinatória de genes variáveis de galinha
... E. coli, a origem de replicação de fagos filamentosos, que contém todos os elementos em cis para a síntese da fita complementar e replicação viral, bem como o sinal de empacotamento do DNA fita simples) como ...
101
Overexpression of Salmonella enterica serovar Typhi recA gene confers fluoroquinolone resistance in Escherichia coli DH5 a
... Escherichia coli DH5a and two recombinant plasmids were ...E. coli DH5a, protein analysis of a crude STM1-A1 extract showed overexpression of a 40 kDa ...E. coli DH5a, the STM1-A11 subclone ...
6
Gag mutations strongly contribute to HIV-1 resistance to protease inhibitors in highly drug-experienced patients besides compensating for fitness loss.
... The absence of effect of MA-CA-SP1 sequences on drug resistance, and their only modest impact on viral fitness, strongly suggests that cleavage events in the NC-SP2-p6 region have a critical impact on resistance and ...
11
MATERIAL AND METHODS Sample collection
... Fungal crude extracts active ( 90%) against the recombinant enzyme trypanothione reductase (TryR) and tested against amastigote forms of Trypanosoma cruzi.. b TryR = recombinant enzyme[r] ...
11
A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide
... We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The ...
7
Melanosis coli
... Melanosis coli denotes brownish discoloration of the colonic mucosa found on endoscopy or histopathologic ...Melanosis coli develops in over 70% of persons who use anthraquinone laxatives (eg cascara ...
8