Reverse transcription
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
... Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) RNA was extracted using the UNIQ-10 column TRIzol kit (Shanghai Sangon Biotech, Shanghai, China) and treated with ...was reverse ...
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A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes.
... involves reverse transcription-based loop mediated isothermal amplification (RT-LAMP) ...by reverse transcriptase, and then isothermal amplification is carried out to increase the amount of total ...
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Detection of Brazilian Hantavirus by Reverse Transcription Polymerase Chain Reaction Amplification of N Gene in Patients with Hantavirus Cardiopulmonary Syndrome
... nested reverse transcription-polymerase chain reaction (RT-PCR) assay for hantavirus using prim- ers selected to match high homology regions of hantavirus genomes detected from the whole blood of hantavirus ...
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Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
... improved reverse transcription-polymerase chain reaction (RT-PCR) protocol for universal detection of flaviviruses by using semi-nested primers that discriminate between DENV serotypes and ...
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The use of reverse transcription-PCR for the diagnosis of X-linked chronic granulomatous disease
... use reverse transcription (RT)-PCR for the analysis of molecular defects responsible for X-linked CGD in eight Brazilian patients and to assess its potential for broader applica- tion to molecular screening ...
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Phosphodiesterase 8a supports HIV-1 replication in macrophages at the level of reverse transcription.
... Rockville, MD, USA) was used. The vector containing the miR- 145-5p overexpression cassette was created by cloning the stem- loop sequence of human miR-145-5p (miRBase accession number MI0000461) including approximately ...
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Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... Melon (Cucumis melo. L) is not only an economically important cucurbitaceous crop but also an attractive model for studying many biological characteristics. Screening appropriate reference genes is essential to ...
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Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut
... Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) is currently the most sensitive techni- que for quantification of low abundance transcripts, and at the same time is suitable for ...
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Rapid and sensitive detection of novel avian-origin influenza A (H7N9) virus by reverse transcription loop-mediated isothermal amplification combined with a lateral-flow device.
... a reverse transcription loop-mediated isothermal amplification combined with a lateral flow device (RT-LAMP-LFD) assay to rapidly detect H7N9 virus was developed and ...
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Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers.
... performed reverse transcription experiments using an LNA-modified RNA template ...III Reverse Transcriptase efficiently transcribed the LNA nucleotides of the template RNA strand by incorporating DNA ...
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Performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples
... and reverse transcription-polymerase chain reaction (RT-PCR) for detecting human respiratory syncytial virus (HRSV) in 306 nasopharyngeal aspirates samples (NPA) was performed in order to assess their ...
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Identification of Suitable Reference Genes for Investigating Gene Expression in Anterior Cruciate Ligament Injury by Using Reverse Transcription-Quantitative PCR.
... The anterior cruciate ligament (ACL) is one of the most frequently injured structures during high-impact sporting activities. Gene expression analysis may be a useful tool for under- standing ACL tears and healing ...
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Comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real-time reverse transcription-PCR assays.
... To evaluate the comparative yields of NP and OP swabs in detecting key respiratory viruses by real-time RT-PCR (qRT- PCR), we conducted a prospective study using paired NP and OP specime[r] ...
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Dengue Virus Detection by Using Reverse Transcription- Polymerase Chain Reaction in Saliva and Progeny of Experimentally Infected Aedes albopictus from Brazil
... and reverse transcriptase-PCR am- plification (RT-PCR) - Viral RNA was extracted from the supernatant of infected cells by using the QIAmp Viral Mini Kit (Qiagen, ...
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A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics.
... using a SuperScript III Platinum One-Step Quantitative RT-PCR system (Life Technologies) or a One Step PrimeScript RT-PCR Kit (Takara/Clontech). A commercial thermal cycler was used to perform real-time RT-PCR, with a ...
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Mapping the sites of latency and reactivation by bovine herpesvirus 5 (BoHV-5) and a thymidine kinase-deleted BoHV-5 in lambs
... (pDx), reverse-transcription-PCR (RT-PCR) for a late viral mRNA (glycoprotein D gene) dem- onstrated reactivation of parental virus in neural (TGs) and lymphoid tissues (tonsils, lymph ...
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Arq. NeuroPsiquiatr. vol.74 número10
... (EV) reverse transcription polymerase chain reaction (RT-PCR) in CSF; this is the first study that tries to establish an acceptable maximum number of RBCs in CSF that does not influence the positivity of ...
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Rev. Bras. Hematol. Hemoter. vol.32 número5
... the reverse transcription reaction, tubes with a 13µL mixture containing 4µl (500ng) total RNA, 1µl Oligo dT primer (50µM), 1µl dNTP (10 mM), and 7µl Rnase-free water, were placed in a dry bath for 5 min at ...
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Noroviruses associated with acute gastroenteritis in a children’s day care facility in Rio de Janeiro, Brazil
... a reverse transcription-polymerase chain reaction (RT-PCR) assay using random primers (PdN6) and specific Ni and E3 primers, directed at a small region of the RNA-dependent RNA polymerase- coding region of ...
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