16S rRNA
Improved detection of bifidobacteria with optimised 16S rRNA-gene based pyrosequencing.
... bacterial 16S rRNA gene followed by pyrosequencing is beginning to reveal the role of the GI microbiome in diverse diseases such as obesity [1], atopic disease [2,3], colonic cancer [4] and necrotizing ...
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Caracterização de rizóbios indicados para produção de inoculantes por meio de sequenciamento parcial do 16S rRNA.
... of 16S rRNA gene of rhizobia strain patterns already classiied with strains recommended for the production of inoculants in Brazil, in order to verify the reliability of partial sequencing of the gene for ...
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Classificação taxonómica de procariotas com base em sequências simuladas do gene 16S rRNA
... de leituras atribuídas a cada táxon dos 5 níveis taxonómicos desde o filo até ao género, e um relatório com diversas estatísticas. Neste trabalho, o sim16S foi utilizado para produzir diversos conjuntos de leituras de 2 ...
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Classificação taxonômica das estirpes de rizóbio recomendadas para as culturas da soja e do feijoeiro baseada no seqüenciamento do gene 16S rRNA.
... Soybean [Glycine max (L.) Merrill] and common bean (Phaseolus vulgaris L.) crops are of economical and social importance in Brazil; their requirement for nitrogen can be supplied by the symbiosis with bacteria belonging ...
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Plastid 16S rRNA gene diversity among eukaryotic picophytoplankton sorted by flow cytometry from the South Pacific Ocean.
... Comparison of the two primers OXY107F vs. PLA491F. It has been suggested that mismatches close to the 5 9 end can result in poor amplification compared to a perfectly matching primer [26]. This is consistent with our ...
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CLUSTOM: a novel method for clustering 16S rRNA next generation sequences by overlap minimization.
... of 16S ribosomal RNA (rRNA) gene sequences from a multitude of natural environments now offers a unique opportunity to study microbial diversity and community ...cluster 16S rRNA sequences ...
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Characidae, Bryconinae) based on the mitochondrial gene 16S rRNA
... Tissues were obtained from adipose fin biopsy and stored in 95% ethanol until their use. One to three individu- als of each species were used for DNA extraction, amplifi- cation and sequencing. Total DNA from ...
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Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system
... the 16S rRNA gene has resulted in a decrease in the generation of the full-length reference sequences that are needed for performing phylogenetic analyses and designing lineage specific PCR primers and ...
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Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies.
... numerous 16S rRNA genes had been collected in databases, the real bacterial world in environmental samples will still be invisible under the current protocol for 16S rDNA ...full-length 16S ...
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Diversidade bacteriana por análise clonal de 16S rRNA e pela hidridação DNA-DNA em...
... Cerca de 96 clones de cada amostra com o tamanho certo do inserto em 12 amostras provenientes de 10 indivíduos com periodontite agressiva foram analisados. Uma seqüência de aproximadamente 500 pares de base foi obtida ...
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Molecular epidemiology of 16S rRNA methyltransferase in Brazil: RmtG in Klebsiella aerogenes ST93 (CC4)
... Ribosomal 16S RNA modification by methyltransferases ...900 16S rRNA methyltransferases (16S-RMTases) and methyltransferase-related ...of 16S-RMTases is crucial to delay the ...
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Biogeochemical typing of paddy field by a data-driven approach revealing sub-systems within a complex environment--a pipeline to filtrate, organize and frame massive dataset from multi-omics analyses.
... BGC type I was statistically independent from the elements tested and from the variation on the FT-IR profile. However, it presented a positive correlation to buckets on the aromatic side of the 1 H-NMR spectra, ...
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Microbiota ileal de frangos de corte submetidos a diferentes dietas.
... gene 16S rRNA do microrganismo probiótico e da microbiota ileal, utilizaram-se os oligonucleotídeos iniciadores: BA338fCG (5´ CGC CCG CGC GCG GCG GGC GGG GCG GGG GCA GCA CGG GGG GAC TCC TAC GGG 3´) e UN518r ...
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Diversidade bacteriana ruminal em bovinos Nelore
... ribosomal 16S rRNA gene that provides resources for identification and also quantification of bacterial ...hypervariable 16S rRNA regions to generate amplicons used for DNA sequencing ...
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SCREENING AND ISOLATION OF HALOPHILIC BACTERIA PRODUCING INDUSTRIALLY IMPORTANT ENZYMES Sumit Kumar
... and 16S rRNA gene analysis found them related to Marinobacter, Virgibacillus, Halobacillus, Geomicrobium, Chromohalobacter, Oceanobacillus, Bacillus, Halomonas and Staphylococcus ...
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MATERIALS AND METHODS Sample collection
... The presence of Lb. manihotivorans was only detected when 16S rRNA genes were hybridized with phylogenetic probes. Using culture-dependent methods, Lacerda et al. (18) showed that Lb. plantarum and ...
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HIGH PREVALENCE OF DNA FROM NON-H. pylori HELICOBACTERS IN THE GASTRIC MUCOSA OF VENEZUELAN PET DOGS AND ITS HISTOLOGICAL ALTERATIONS
... Non-H. pylori helicobacters (NHPH) have been demonstrated as gastric spiral-shaped bacteria in specimens obtained from dogs; however, their roles in the pathogenesis of upper gastrointestinal disease have not yet been ...
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Clonal multidrug-resistant Corynebacterium striatum within a nosocomial environment, Rio de Janeiro, Brazil
... by 16S rRNA and rpoB gene ...reactions. 16S rRNA gene was amplified using universal primers pA (5’-AGAGTTTGATCCTGGCTCAG) and pH (5’-AAG- GAGGTGATCCAGCCGCA), as described by Watts et ...The ...
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Metagenomic identification of active methanogens and methanotrophs in serpentinite springs of the Voltri Massif, Italy
... ESOM Bin 2 (42% complete, 12% contamination) includes sequences that were classified as Comamonadaceae (43% of taxonomic markers) and Xanthomonadaceae (27% of taxonomic markers) (File S6). Both of these bacterial ...
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Assessment of microbial communities associated with fermentative–methanogenic biodegradation of aromatic hydrocarbons in groundwater contaminated with a biodiesel blend (B20)
... Noticeable BTEX removal occurred at 0.7 year, considerably faster than under natural attenuation conditions (with a noticeable onset of BTEX biodeg- radation after 2.5 years) (Ramos et al. 2013). BTEX removal was ...
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