T cells - CD69
Increased frequency and compromised function of T regulatory cells in systemic sclerosis (SSc) is related to a diminished CD69 and TGFbeta expression.
... CD3+ cells were isolated from PBMCs using CD3 microbeads according to manufacturer’s protocol (Miltenyi ...CD3+ cells were re-suspended in 100 m l buffer (PBS + 1% BSA) on ...isolation, cells were ...
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miR-20a inhibits TCR-mediated signaling and cytokine production in human naive CD4+ T cells.
... and CD69 expression, overexpression of miR- 20a did not affect T-cell ...+ T-cell proliferation upon antigen stimula- tion ...of T-cell pro- liferation but rather in the cytokine production in ...
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T cells in sputum of asthmatic patients are activated independently of disease severity or control
... or CD69 on sputum lymphocytes and the control of ...of CD69 on sputum CD8+ T lymphocytes showed a trend towards being significantly increased in moderately severe asthma, when controlled patients were ...
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Effect of immunoglobin-like transcript 7 cross-linking on plasmacytoid dendritic cells differentiation into antigen-presenting cells.
... stimulate T cell activation and proliferation, we co- cultured purified pDC with autologous T cells in presence of different combinations of TLR9L, XL-ILT7 and the T cell superantigen ...
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Phenotypic and functional aspects of CD8 + T cells in atopy: from populations to cells
... Sputum cells and PBMC were washed in PBS containing ...dark. Cells were then washed, resuspended in PBS and acquired using a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA, ...positive ...
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Mesenchymal stem cells promote the sustained expression of CD69 on activated T lymphocytes : roles of canonical and noncanonical NF B signalling
... stem cells (MSCs) are known to induce the conversion of activated T cells into regulatory T cells in ...marker CD69 is a target of canonical nuclear factor kappa-B (NF-B) ...
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Obesity impairs γδ T cell homeostasis and antiviral function in humans.
... Vγ9Vδ2 T cells recognize and are activated by non-prenylpeptidic antigens such as those in the mevalonate pathway ...Vγ9Vδ2 T cells isolated from obese subjects, PBMC were stimulated in vitro ...
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Filter-Dense Multicolor Microscopy.
... Immunofluorescence microscopy—microscopic detection of proteins using fluorochrome-la- beled antibodies—is a powerful approach to analyze the spatial localization of proteins in tis- sues. The technique can be used for ...
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Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients.
... NK cells bearing self-specific KIR remain elusive; we can only hypothesize that HLA-presented DENV-2 peptides could modulate KIR/HLA interaction as previously shown in HIV-1 infected patients ...
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Rev. Bras. Reumatol. vol.56 número1
... tri-functional cells relative to all cells produc- ing any one of the analyzed cytokines, we did find a significant difference in cultures stimulated with PMA/Io, where samples from patients with A-SLE had ...
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Identification of a transient lymphoid population in the murine epidermis
... Langerhans cells (LCs) can be found on both mouse and human epidermis and have been distinguished from other cells by the expression of langerin and CD1a, respectively 16,20 ...naїve T cells ...
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Microbial Translocation and B Cell Dysfunction in Human Immunodeficiency Virus Disease
... B cells and reduced production of antigen-specific antibody is seen in the majority of chronic HIV infection even though the humoral system is subject to repeated and long-term stimulation through TLR agonists ...
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HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT).
... on T cells and a major target for immune checkpoint ...+ T cells (S9 Fig), indicating that PD-1 is also a target of ...ATL cells and HTLV-1 infected cells of HAM/TSP patients ...
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Regulatory T and B cells in asthmatic women
... Métodos: Se incluyeron cuatro grupos de mujeres para este estudio: mujeres embarazadas en su tercer trimestre, asmáticas (n = 24) y sanas (n = 43), y mujeres no embarazadas, asmáticas (n = 33) y sanas (n = 35). Las ...
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Expansion of NK cells and reduction of NKG2D expression in chronic lymphocytic leukemia. Correlation with progressive disease.
... CD8 T cells at diagnosis. The relative number of CD8 T cells at diagnosis was associated with time to treatment, suggesting that CD8 T cells may modify disease ...CD8 T ...
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High efficiency of antiviral CD4(+) killer T cells.
... Target cells were distinguished from recipient cells by flow cytometric analysis based on CFSE fluorescence, in some cases also congenic markers, and further stratified according to MHC-II expression ...
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HIV-1 infection on Follicular Helper T cells
... The innate immune system is the first line of host defense comprising multiple mechanisms and cells (macrophages, DCs and natural killer cells (NKs)) that provide immediate protection against infection. It ...
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A convenient model of severe, high incidence autoimmune gastritis caused by polyclonal effector T cells and without perturbation of regulatory T cells.
... by T cells with a single specificity as observed in TCR transgenic mice does not resemble normal physiological ...of T cells from H/Ka 2/2 mice, a ...
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Focusing on the epigenetic control of regulatory T cells
... Diferenças nos níveis de metilação da região TSDR contribuem para a distinção entre células Treg e linfócitos T convencionais (Tcon). A par da região TSDR, verificou-se que o gene CAMTA1, localizado no cromossoma ...
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The role of CD8+ T cells during allograft rejection
... these cells as preformed and prepacked chemokines ...immune cells in the al- lograft is essential for the pathogenesis of tissue ...of cells mediated by selectins, followed by firm arrest on the ...
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