Two-phase aqueous systems
Forças motrizes da transferência de vermelho 40 e vermelho ponceau 4R em sistemas aquosos bifásicosDriving forces of transfer of allura red and red ponceau 4R in two-phase aqueous systems
... El estudio del comportamiento de partición en SABS usando los colorantes Rojo 40 y Rojo Ponceau 4R como sondas moleculares dieron conocimientos experimentales s[r] ...
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Antibodies purification using aqueous micellar two-phase systems
... mandatory. Aqueous micellar two-phase systems (AMTPS) are a special type of aqueous two-phase systems that comprise micellar solutions of surfactants for ...
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Enhanced performance of polymer-polymer aqueous two-phase systems using ionic liquids as adjuvants towards the purification of recombinant proteins
... requires additional processing steps envisaging the isolation and solubilization of the target protein. Considering the works developed by Prazeres [4] and Mukherjee [5] research groups on the solubilization of IFNα-2b ...
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Progress in the Development of Aqueous Two-Phase Systems Comprising Ionic Liquids for the Downstream Processing of Protein- Based Biopharmaceuticals
... polymer-salt systems as a way to increase the purification factors of a clinically relevant enzyme - ...this phase, resulting in a decrease of contaminant proteins and higher purification ...
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Recovery of pectinases from Aspergillus niger using aqueous two-phase systems / Recuperação de pectinases de Aspergillus niger usando sistemas aquosos bifásicos
... The systems were weighed into centrifuge tubes (50 mL), homogenized at room temperature during 15 min and for phase separation the samples were centrifuged (centrifuge MPW-351R, Warsaw, Poland) at 4,000 x g ...
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Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction
... Aqueous two-phase systems (ATPS) are formed by mixtures of chemical species that, under certain conditions, separate into two immiscible phases, with water as the major component in ...
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Partition of Glucose Oxidase from Aspergillus niger in Aqueous Two-Phase Systems Based on Salt and Polyethylene Glycol
... The results showed a increase of K with temperature and maximum partition coefficient (6.9) at temperature 35 0 C. At 40 0 C there was sharp decline. The effect of temperature is quite complex because the phase ...
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STUDY OF ALPHA-AMYLASE OBTAINED BY SOLID STATE FERMENTATION OF CASSAVA RESIDUE IN AQUEOUS TWO-PHASE SYSTEMS
... phosphate salts were determined in this work. The stock solutions of polyethylene glycol (50% w/w) and potassium phosphate buffer (20% w/w) were prepared before conducting the experiments. The pH of the PEG solution was ...
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Enzymatic hydrolysis of sugarcane bagasse in aqueous two-phase systems (ATPS): exploration and conceptual process design
... literature. Aqueous two-phase systems (ATPS) can be applied to design an extractive reaction due to their capacity to partition solutes to different phases in such a ...studied two ...
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Partição de clorofila e betalaína utilizando sistemas aquosos bifásicosPartition of chlorophyll and betalain using aqueous two-phase systems
... Termodinamicamente, foi observado que para sistemas formados por PEG 1500 e todos os sais estudados, o processo de transferência do corante foi exotérmico Em sistemas formados por PEG 40[r] ...
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Bioconversion of apigenin-7-O-β-glucoside in aqueous two-phase system
... top phase apigenin yield of 108% (Table ...that phase volume ratio increased but content of apigenin in the top phase decreased when phosphate buffer was used, which resulted in lower yield of ...
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Purification of C-phycocyanin from Spirulina platensis in Aqueous Two-Phase Systems using an Experimental Design
... that allow for refinement and a better understanding of the system under the study (Montgomery 1991; Barros Neto et al. 1995). The present work aimed to study and optimize the purification of the C-phycocyanin extracted ...
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Determinação das forças motrizes de formação e partição em sistemas aquosos bifásicos macromolécula + salDetermination of the driven forces in the formation and partition in macromolecule + salt aqueous two-phase systems
... of aqueous two-phase systems (ATPS) at the industrial level requires systems formed by non-toxic substances to decrease the negative impact on the ...the phase diagrams increased ...
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LIQUID-LIQUID EQUILIBRIUM DATA, VISCOSITIES, DENSITIES, CONDUCTIVITIES, AND REFRACTIVE INDEXES OF POLY (VINYL PYRROLIDONE)+ TRI-SODIUM CITRATE + UREA AQUEOUS TWO-PHASE SYSTEMS AT DIFFERENT PH
... polymer-rich phase (and TLL) behaved in a slightly shorter ...the aqueous single-phase system of PVP (K30) (p) + tri-sodium citrate (s) + urea (u) + water) at ...
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Novos sistemas aquosos bifásicos aplicados no preparo de amostras alimentícias para a determinação de corantes sintéticosNew aqueous two-phase systems applied in the food sample preparation for the determination of synthetic dyes
... Artificial dyes are widely used in the food industry with the aim of making the food more appetizing. However, artificial dye consumption is associated with several deleterious effects on human health, necessitating ...
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Thermoreversible (Ionic-Liquid-Based) Aqueous Biphasic Systems
... proteins. Aqueous solutions of each biomolecule were prepared at the concentra- tions of 1, 2 and 3 g·L −1 ...the phase diagram of [N 11[2(N11)]0 ][C 1 CO 2 ]-based ABS: 6 wt % [N 11[2(N11)]0 ][C 1 CO 2 ] + ...
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Application of aqueous two-phase systems for the development of a new method of cobalt(II), iron(III) and nickel(II) extraction: A green chemistry approach
... Only few reports found in the literature focus on the study of ion transfer in ATPS, but almost all refer to systems formed with PEO and different electrolytes. To the best of our current knowledge, only ...
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Novas descobertas sobre a partição de corantes fenotiazínicos em sistemas aquosos bifásicosNew insights about the partition of phenothiazines dyes in aqueous two-phase systems
... Aqueous two-phase systems (ATPSs) have proven to be an efficient and environmentally safe method for extraction of chemical ...these systems is still little ...enriched phase, ...
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Copper recovery from ore by liquid–liquid extraction using aqueous two-phase system
... A phase split results in a polymer-enriched top phase and an electrolyte-enriched bottom ...these systems have a high content of water in both phases ...These systems have been used for the ...
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An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems
... the two-phase ...bottom phase – from the first – was transferred into a tube containing 5 mL of a pure top phase (from a previously prepared phase system obtained under identical ...
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