[PDF] Top 20 Application of aqueous biphasic systems for the separation of amino acids and proteins

... explore the differences in physicochemical properties of target compounds and contaminants present in the original media ...[2]. Separation and purification stages in ... See full document

... bioconversion; and product ...stages, the use of organic and volatile solvents, and high energy demands to achieve the desired separation or purification level 4 ...for ... See full document

... Partitioning of cytochrome c and azocasein. Cytochrome c and azocasein were studied as represent- ative examples of added-value ...proteins. Aqueous solutions of each ... See full document

... to the World Health Organization, the occurrence of antibiotic-resistant microorganisms is nowadays one of the most severe threats to global health, food security, and ...world ... See full document

... In the past decade, remarkable advances in the production and use of antibodies as therapeutic drugs and in research/diagnostic fields have led to their recognition as value-added ... See full document

... with the solubility effects observed for other biomolecules such as polymers, 61 charged polypeptides 62 and amino ...sity of biomolecules, and are known to stabilize a variety of ... See full document

... For the design of ABS as extraction and purification processes, their phase diagrams and respective tie-lines are ...set of conditions, such as temperature and pH ...any ... See full document

... processes, aqueous biphasic systems (ABS) have been recognized as an economic and efficient downstream processing tool to be used in the separation and purification ... See full document

... at the development of self-buffering and benign extraction/separation processes, this work reports a novel class of aqueous biphasic systems (ABS) composed ... See full document

... Recently amino acids became the topic of many studies due to their biological and industrial ...on the solubility of several amino acids and diglycine ... See full document

... ABSs and the characteristics and properties of the coexisting ...all the phase formers are quite important when applied DES-based ABS in the partition of different ... See full document

... interactions and interacting amino acids residues provide more details about the docking affinity on protein ...this, the proteins were classified into two categories: ... See full document

... on the tailoring ability of ILs, either for the solvation of a wide array of compounds or to extract the most diverse biomolecules from aqueous media [73], in this work, ... See full document

... determined and their ability to extract biomolecules was evaluated with two amino acids, namely tyrosine and ...tryptophan. The results obtained indicate that the amino ... See full document

... type of LLE system that do not use toxic or volatile organic ...One of the aqueous phases is enriched in one of the solutes, while the coexisting phase is mainly formed by ... See full document

... TWO-PHASE SYSTEMS: FUNDAMENTALS AND APPLICATIONS FOR PARTITIONING/PURIFICATION OF ...By the year 2005 the world biochemical market will reach an estimated $ 100 billion and ... See full document

... In the past decade, aqueous biphasic systems (ABS) composed of ILs and a wide number of salts, carbohydrates, polymers, or amino acids have been extensively ... See full document

... as amino acids, their derivatives, and many other ...efficient separation and resolution, as well as relatively easy method development, small sample volume, rapid analysis and ... See full document

... for the extraction and purification of egg yolk antibodies and the most used involve protein precipitation with ammonium sulphate, dextran sulphate or polyethylene glycol (PEG) ... See full document

... conversion of 38 to 5-fluorouracil (5-FU) following systemic delivery decreases its therapeutic ...efficacy. The mechanism of action of 38 and its major metabolite is well ... See full document