[PDF] Top 20 Biological effects of cigarette smoke in cultured human retinal pigment epithelial cells.
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Biological effects of cigarette smoke in cultured human retinal pigment epithelial cells.
... extracts, cells were washed twice with ice-cold PBS, collected, and lysed in three times packed cell volumes of low-salt hypotonic cell lysis buffer [20 mM HEPES pH ...uC) in a microfuge, the ... See full document
9
Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells.
... formation of the human ovarian teratocarcinoma cell line PA-1 [20] occurs in an agar medium, since PA-1 cells could mimic possible malignant cells derived from ...PA-1 cells ... See full document
9
Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium
... 2006). Cigarette smoke is a complex mixture of over 4700 identified constituents that include numerous reactive substances such as a large quantity of reactive aldehydes, free radical species ... See full document
10
Investigating the epigenetic effects of a prototype smoke-derived carcinogen in human cells.
... class of carcinogenic compounds present in tobacco smoke, as well as in numerous other sources, including occupational, environmental, ...its biological effects in vivo ... See full document
12
Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle
... activity of epithelial cells in the normal breast throughout the menstrual cycle [3-8], an underlying limitation in these studies is the concurrent use of oral contraceptives by ... See full document
8
Effects of cigarette smoke inhalation and coffee consumption on bone formation and osseous integration of hydroxyapatite implant
... one of the most popular beverages and it is highly appreciated among smokers, who often drink a cup of coffee before smoking or ...found in coffee, and nicotine, the toxic addicting agent in ... See full document
6
Ocular masquerade syndrome due to intraocular lymphoma - two forms of retinal pigment epithelium involvement: case reports
... curring in 31 eyes from 17 patients with primary intraocular ...disturbances of the RPE were: granularity (61%); blockage (55%) and late staining (45%), more than one could be found in the same ... See full document
5
GLUT1 activity contributes to the impairment of PEDF secretion by the RPE
... Detection of GLUT1 expression by western blot: Whole cell proteins were extracted in cold RIPA buffer (50 mM Tris-HCl pH ...isolation of the membrane/soluble fractions, cells were extracted ... See full document
10
Colonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment.
... Cigarette smoke (CS) protects against intestinal inflammation during ulcerative ...aim of this study was to assess the effects of CS on experimental colitis and to characterize the ... See full document
13
Effects of maternal smoking on the placental expression of genes related to angiogenesis and apoptosis during the first trimester.
... termination of a pregnancy at 6–8 weeks of ...age of the fetus (in ...out in the Department of Obstetrics and Gynecology at Showa University School of Medicine (Tokyo, ... See full document
7
Cytotoxic Effects of Zoledronic Acid on Human Epithelial Cells and Gingival Fibroblasts
... activity of SDH enzyme, which is a measure of cellular (mitochondrial) respiration, and can be considered as the metabolic rate of ...h of incubation of the cells in ... See full document
8
Characteristics of Human Natal Stem Cells Cultured in Allogeneic Medium
... population of DP-MSC is enclosed in natal teeth and neonatal ...series of teeth (>90%) (3), which tend to appear in place of lower primary central incisors ...1:3000 of ... See full document
8
Red blood cells protect albumin from cigarette smoke-induced oxidation.
... Oxidation of HSA Cys34 thiol is partially reversible in the presence of ...reversible in the presence of 1–2 mM DTT. Fig. 2A shows the detection of the Cys34 free sulfhydryl ... See full document
13
Sao Paulo Med. J. vol.127 número1
... hydration of the histological sections, they were then subjected to blocking of endogenous peroxidase, and an- tigen recovery in a steamer (T-Fall, Dijon, France) with 10 mM citrate buffer at pH ... See full document
6
HDACs and HDAC inhibitors
... inhibition of HDAC6 by LBH-589 and M344 resulted in the highest fold increase of full-length transcripts, even when compared to inhibition of multiple ...diversity of functions for ... See full document
9
Behavior of cardiac variables in animals exposed to cigarette smoke
... to cigarette smoke resulted in effects on the rat’s heart, whose detection depended on the method used for functional ...studied in the preparations with isola- ted papillary muscle, no ... See full document
4
Gene expression profile and toxic effects in human bronchial epithelial cells exposed to zearalenone.
... found in cereal crops such as maize, wheat and ...present in air, little is known about the health risk or the molecular basis of action when lung cells are exposed to ...purpose of ... See full document
19
Rev. Col. Bras. Cir. vol.41 número1
... model of exposure to tobacco burning (cigarette) products to assess the effects of its chronic use in relation to cancers of the ...products in a semi-open chamber to ... See full document
4
Giardia duodenalis Surface Cysteine Proteases Induce Cleavage of the Intestinal Epithelial Cytoskeletal Protein Villin via Myosin Light Chain Kinase.
... results in delayed epithelial apoptosis in vitro, and its deletion in vivo enhances susceptibility to dextran sodium sulfate (DSS)- induced colitis due to increased rates of IEC ... See full document
28
Expression of activated PIK3CA in ovarian surface epithelium results in hyperplasia but not tumor formation.
... [43]. In p53 null murine OSE cells, enforced expression of the PI3-kinase downstream mediator, Akt, in cooperation with mutant K-ras, induces the OSE cell transformation ...tumors in ... See full document
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