[PDF] Top 20 Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons.
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Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons.
... described in Koch et al [11] to three ES cell lines and six different iPSC lines originating from fibroblasts or from radial glia-like neural stem (NS) cells derived from ... See full document
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Fibroblasts derived from human pluripotent stem cells activate angiogenic responses in vitro and in vivo.
... induced pluripotent stem cells (hESC/hiPSC) are promising cell sources for the derivation of large numbers of specific cell types for tissue engineering and cell therapy ...fibroblasts ... See full document
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Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.
... therapy like cell replacement ...precursor cells (NPCs). However, these cells have a low proliferation rate and are therefore not ideal for transplantation ...[2]. Human embryonic stem ... See full document
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Einstein (São Paulo) vol.12 número1
... proliferation of the two cell types was significantly increased after laser irradiation when compared with the non-irradiated control ...the cells, which was also observed in the present study ... See full document
7
Male germ-like cell differentiation potential of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells in co-culture with human placenta cells in presence of BMP4 and retinoic acid
... level of pluripotency ...performed in triplicate by SYBR green real- time master mix (Takara, Japan) in Rotor gene 3000 system (Corbett, ...well of the PCR reactions l of SYBR ... See full document
9
Making human neurons from stem cells after spinal cord injury.
... quantities of motor neurons will be benefi cial for treating neurodegenerative diseases such as ALS (amyotrophic lateral ...neuron production is obtained after other experimental types of ... See full document
3
Motoneuron differentiation of induced pluripotent stem cells from SOD1G93A mice.
... properties of both ALS-iPS and C57-iPS derived motoneurons in ...RNAs of motoneurons derived from ALS-iPS and C57- iPS cells were extracted using TRIzol (Invitrogen) ...Time ... See full document
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Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.
... RE19 from S7) carried fewer mutations, of which a subset was shared between them as well as with EPCs from the same ...None of the shared SNVs were detected in the corresponding ... See full document
15
Design and Characterization of Bioengineered Cancer-Like Stem Cells.
... development of various malignant tumors from the stem cells will indeed be possible, this may require additional studies to further investigate the specific role of different microen- ... See full document
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Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.
... dehydrated in graded ethanols, cleared in xylene, and paraffin-embedded ...series of ethanols, and washed in ...X-100 in phosphate-buffered saline, pH ...antibodies in blocking ... See full document
11
Stepwise development of hematopoietic stem cells from embryonic stem cells.
... promoter from Cytomegalovirus (CMV); the rabbit beta-globin 2nd intron; human HOXB4 cDNA; an internal ribosome entry site (ires); EGFP cDNA; and a polyA ...amplified in E. coli Stabl2 cells ... See full document
10
Figueiredo a Leal Santos Baptista de
... expansão in vitro assim como a possibilidade de manipulação genética, permitindo então a criação artificial de diferentes linhagens celulares com marcadores específicos (Nishikawa et ...culturas in ... See full document
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The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method
... involved in reprogram- ming to produce iPSCs remain largely ...an in-depth analysis of the biological functions of differentially expressed genes in all 20 iPSC lines ...difference ... See full document
8
Induced pluripotent stem cell modeling of Gaucher's disease: what have we learned?
... number of approaches to ameliorate symptoms or cure the disease have been explored experimentally and/or clinically, including enzyme replacement therapy (ERT), substrate reduction therapy (SRT), chaperone ... See full document
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Reprogramming primordial germ cells into pluripotent stem cells.
... germ cells (PGCs) are the embryonic precursors of the germ cell lineage, which are restricted to form only sperm and eggs following their specification from pluripotent epiblast ...determinant ... See full document
8
Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells.
... commitment of a specialized cell type from induced pluripotent stem cells (iPSCs) without contamination from unknown substances is crucial to their use in clinical ... See full document
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Expression and secretion of human recombinant LIF by genetically modified mammalian cells
... для куль ти ви ро ва ния ме зен хи маль ных ство ло вых кле ток (МСК), улуч ша ет их осте о ген ную диф фе - рен ци ров ку [6, 7], до бав ля ет ся в сре ды для диф фе - рен ци ров ки МСК в кар ди о ми о ци ты [8, 9] и ... See full document
6
Differentiation of human pluripotent stem cells to the neuronal dopaminergic fate
... portion of the dorsal ectoderm forms the neural ectoderm and its cells become distinguishable by their columnar appearance; this region of the embryo is called the neural plate, which ultimately ... See full document
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Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
... iPS cells cultured on various substrates were differentiated into three germ layer- derived lineages as described previously with minor modifications ...201B6 cells maintained on R-Fc, NC-Fc, or Matrigel ... See full document
17
Culture conditions affect cardiac differentiation potential of human pluripotent stem cells.
... positive cells could be detected in long-term culture of hPSCs on Matrigel matrix together with mTeSR1 medium than in MEF and SNL feeder cell ...expression of PAX-6, Musashi and NF-68 ... See full document
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