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[PDF] Top 20 Characterization of pyruvate uptake in Escherichia coli K-12.

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Characterization of pyruvate uptake in Escherichia coli K-12.

Characterization of pyruvate uptake in Escherichia coli K-12.

... monocarboxylate pyruvate is an important metabolite and can serve as sole carbon source for Escherichia ...specific pyruvate transporters have been identified in two bacterial species, ... See full document

7

Characterization of two new CTX-M-25-group extended-spectrum β-lactamase variants identified in Escherichia coli isolates from Israel.

Characterization of two new CTX-M-25-group extended-spectrum β-lactamase variants identified in Escherichia coli isolates from Israel.

... Indianapolis, IN, USA) according to the manufacturer’s protocols. Replicon types of all unique plasmids were identified by PCR-based replicon typing as described previously ...E. coli A15 strain as a ... See full document

7

Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli

Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli

... used in this work involved the fusion of ...tag in order to allow simple one-step purification using Ni- NTA ...derivation of this method using Ni ion- chelating magnetic beads to increase ... See full document

6

Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion

Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion

... deficient in lon and ompT proteases that degrade abnormal and outer membrane pro- teins, respectively, provides higher levels of expression, as well as a tighter control of protein expression, by ... See full document

8

Representational difference analysis between Afa/Dr diffusely adhering Escherichia coli and nonpathogenic E-coli K-12

Representational difference analysis between Afa/Dr diffusely adhering Escherichia coli and nonpathogenic E-coli K-12

... relation of E. coli C1845 to uropathogenic iso- lates, we investigated the presence of C1845-specific sequences in the Afa/Dr strain IH11128, recovered from a patient with ...E. coli ... See full document

9

Adaptive response of Escherichia coli K-12 strains to cadmium-induced oxidative stress.

Adaptive response of Escherichia coli K-12 strains to cadmium-induced oxidative stress.

... enzyme of the glycolytic metabolism that catalyzes the interconversion of PEP and ...2-phospho-D-glycerate. In E. coli, roughly one-tenth of the total enolase is sequestered in a ... See full document

166

Molecular and Phenotypic Characterization of Escherichia coli O26:H8 among Diarrheagenic E. coli O26 Strains Isolated in Brazil

Molecular and Phenotypic Characterization of Escherichia coli O26:H8 among Diarrheagenic E. coli O26 Strains Isolated in Brazil

... Prevalence of other gene ...performed in accordance with the recommendations of the manufacturer, using EvaGreen DNA binding dye (Biotium ...cycles of 95°C for 15 s and 60°C for 1 min ... See full document

8

Geniposide regulates glucose-stimulated insulin secretion possibly through controlling glucose metabolism in INS-1 cells.

Geniposide regulates glucose-stimulated insulin secretion possibly through controlling glucose metabolism in INS-1 cells.

... aliquot of 10-20 μg protein from each cell extract was loaded on a 10% SDS-PAGE ...diluted in a blocking solution and incubated with the membrane for indicated times as described previously ... See full document

9

Pyrosequencing-based analysis reveals a novel capsular gene cluster in a KPC-producing Klebsiella pneumoniae clinical isolate identified in Brazil

Pyrosequencing-based analysis reveals a novel capsular gene cluster in a KPC-producing Klebsiella pneumoniae clinical isolate identified in Brazil

... element of the IS3 family ...found in this ...occurrence of mutations leading to premature stop codons and/or frameshifts might have rendered this transposase ...protein of a Mu-like prophage ... See full document

12

Caracterização epidemiológica, molecular e perfil de resistência aos antimicrobianos de Escherichia coli isoladas de criatórios suínos do sul do Brasil.

Caracterização epidemiológica, molecular e perfil de resistência aos antimicrobianos de Escherichia coli isoladas de criatórios suínos do sul do Brasil.

... Nenhum isolado foi positivo para o gene de F6, dado diver- gente do observado por Martins et al. (2000), que encontrou 35,7% de amostras positivas para este fator. O mesmo autor tam- bém encontrou índice de 1,2% para F5, ... See full document

5

Distinct pathotypes of O113 Escherichia coli strains isolated from humans and animals in Brazil

Distinct pathotypes of O113 Escherichia coli strains isolated from humans and animals in Brazil

... observed in 32 (84%) of the 38 strains studied, and the AA pattern was found in 29 (76%) of them (Table ...occurrence of a high percentage of AA among the studied strains, ... See full document

3

Efficient Expression and Purification of Recombinant Human Enteropeptidase Light Chain in Esherichia coli

Efficient Expression and Purification of Recombinant Human Enteropeptidase Light Chain in Esherichia coli

... E. coli BL21 (DE3) by a 40 s heat shock at ...clones of E. coli BL21 (DE3) containing recombinant plasmid then grown overnight in LB medium containing 100 mg/mL ampicillin at 37°C at 180 ... See full document

6

Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

... characterizations of PEPEC showed that they lack the virulence factors of ETEC but present porcine attaching and effacing- associated (paa) nucleotide sequences, associated with the ability to produce ... See full document

8

Expression of Grapevine leafroll-associated virus 3 Coat Protein Gene in Escherichia coli and Production of Polyclonal Antibodies

Expression of Grapevine leafroll-associated virus 3 Coat Protein Gene in Escherichia coli and Production of Polyclonal Antibodies

... Grapevine leafroll-associated virus 3 (GLRaV-3), a principal espécie viral do complexo do enrolamento da folha da videira (Vitis spp.), causa reduções no rendimento e na qualidade da uva. O gene da proteína capsidial foi ... See full document

5

Bacterial strains and determination of toxins and CFs

Bacterial strains and determination of toxins and CFs

... Enterotoxigenic Escherichia coli (ETEC) is recognized as the main cause of bacterial diarrhoea among children in Asia, Africa and Latin America but less investigated in ...patterns ... See full document

6

Functional studies on BolA and related genes:

Functional studies on BolA and related genes:

... effects of mutations that cause earlier aging in mother cells (Ackermann et ...dispersal of the cytoplasmic material, therefore supposedly dividing equally the damaged constituents, cell poles and ... See full document

246

Virulence factors of Escherichia coli isolated from diarrheic calves

Virulence factors of Escherichia coli isolated from diarrheic calves

... E. coli strains (STEC) is similar to the Shiga-toxin produced by Shigella dysenteriae type ...E. coli producing Stx 1 and/or 2, causes the hemorrhagic colitis (HC) and the hemolytic-uraemic syndrome (HUS) ... See full document

6

Antimicrobial Non-Susceptibility of Escherichia coli from Outpatients and Patients Visiting Emergency Rooms in Taiwan.

Antimicrobial Non-Susceptibility of Escherichia coli from Outpatients and Patients Visiting Emergency Rooms in Taiwan.

... increase in non-susceptibility to several broad spectrum antimicrobial agents, including cefotaxime and ciprofloxacin, occurred in ...E. coli from community settings in ...only in ... See full document

17

Effects of combination of magnesium and zinc oxide nanoparticles and heat on Escherichia coli and Staphylococcus aureus bacteria in milk

Effects of combination of magnesium and zinc oxide nanoparticles and heat on Escherichia coli and Staphylococcus aureus bacteria in milk

... strains of methicillin resistant ...combination of these nanoparticles results in synergism ...mechanism of nanomaterials on microorganisms has not been established, several studies suggest ... See full document

8

Adhesin-Encoding Genes from Shiga Toxin-Producing Escherichia coli Are More Prevalent in Atypical than in Typical Enteropathogenic E. coli

Adhesin-Encoding Genes from Shiga Toxin-Producing Escherichia coli Are More Prevalent in Atypical than in Typical Enteropathogenic E. coli

... combinations of the genes studied were found among diverse aEPEC and tEPEC serotypes (Tables 3 and ...tribution of lpfA in tEPEC agreed with our previous study (26); however, this is the first ... See full document

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