[PDF] Top 20 Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
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Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... 4-alpha) in Oryza sativa; CUL (Cullin), FPGS (Folylpolyglutamate synthase), LUG (Leunig), MEP (Membrane protein) and UBCP (Ubiquitin carrier protein) in Zea mays; CAC (Clathrin adaptor complex) and TIPS-41 ... See full document
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Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.
... interpretation of qRT-PCR data is the necessity of a stable, constitutively-expressed reference gene which can be used as a standard measure for comparing one sample to another ...This ... See full document
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Importance of suitable reference gene selection for quantitative RT-PCR during ATDC5 cells chondrocyte differentiation.
... quantify gene transcripts, real-time quantitative reverse transcription-polymerase chain reaction (qPCR) will undoubtedly play a pivotal role in deciphering the cellular and molecular ... See full document
7
Selection of suitable housekeeping genes for real-time quantitative PCR in CD4(+) lymphocytes from asthmatics with or without depression.
... The expression analysis for all 9 genes was performed using an FTC 2000 qPCR system (Funglyn Biotech Inc, Scarborough, Canada), PCR primers and TaqMan probes were obtained from Shanghai biological ... See full document
9
Selection and assessment of reference genes for quantitative PCR normalization in migratory locust Locusta migratoria (Orthoptera: Acrididae).
... The expression stability of selected internal control genes was evaluated with four statistical algorithms (the delta Ct approach, geNorm, Bestkeeper, and ...combination of different ... See full document
16
Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
... exons of human cDNA sequences. To evaluate the possibility of genomic amplification, minus-RT PCR were performed using DNAse treated RNA in the same dilution used for the cDNA ... See full document
11
Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria.
... group of photosynthetic prokaryotes, with a large morphological diversity including unicellular, colonial and filamentous ...specialized in photosynthesis (vegetative cells) and in nitrogen fixation ... See full document
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Real Time PCR and Importance of Housekeepings Genes for Normalization and Quantification of mRNA Expression in Different Tissues
... The real time PCR technique is effective to measure the absolute transcript and provides valuable quantitative information on gene expression from different sources and samples ... See full document
12
Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.
... estimate gene expression stability, the comparison of the different methods could be the best way to identify the best reference genes to be ...potential reference genes. ... See full document
9
Identification of Suitable Reference Genes for Investigating Gene Expression in Anterior Cruciate Ligament Injury by Using Reverse Transcription-Quantitative PCR.
... results of the present study indicate that the use of suitable reference genes for reliable gene expression evaluation by RT-qPCR should consider the type of ... See full document
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Validation of reference genes for quantitative RT-PCR normalization in Suaeda aralocaspica, an annual halophyte with heteromorphism and C4 pathway without Kranz anatomy
... stress-responded genes, high throughput gene expression analysis should be considered (Delporte et ...transcription quantitative real-time PCR (qRT- PCR) is one ... See full document
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Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... P-glycoprotein expression levels was higher in resistant than in susceptible strain had been previously found by Lou et al [21] using Western blotting and Vanadate-sensitive ATPase ... See full document
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Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).
... Gene expression analysis plays a major role in understanding of the complex regulatory net- works and identification of genes relevant to new biological processes ...measurement, ... See full document
16
Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.
... Quantitative real-time reverse transcription polymerase chain reaction (qPCR) is an efficient, specific, and reproducible method for quantifying transcript expression levels, and is widely ... See full document
10
Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... One of the methodologies used to determine expression levels by RT-qPCR compares the gene of interest with reference genes, whose expression does not change ... See full document
8
Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
... analysis of gene expression [16, 23, 24]. However, to the best of our knowledge, a systematic study on endogenous control genes expression under different stress conditions has ... See full document
20
Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... appropriate reference genes is essential to reverse transcription quantitative real-time PCR (RT-qPCR), which is key to many studies involving gene ... See full document
11
Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
... searches of the literature a group of previously used control genes in plants was selected ...candidate reference genes were identified after performing a TBLATN among the ... See full document
12
Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars
... de genes de referência em cana-de-açúcar sob estresse ...usando PCR tempo real para os genes α-tubulina, β-tubulina, β-actina, ciclofilina, fator 1 de alongamento eucariótico, gliceraldeído ... See full document
7
Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes
... Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in re- sponse to gastrointestinal nematode ...infection. By ... See full document
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