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[PDF] Top 20 No control genes required: Bayesian analysis of qRT-PCR data.

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No control genes required: Bayesian analysis of qRT-PCR data.

No control genes required: Bayesian analysis of qRT-PCR data.

... qPCR analysis on dilution series data made relative quantification practically equivalent to the other flavor of qRT-PCR analysis, absolute quantification ...(‘‘cycle of ... See full document

12

Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea.

Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea.

... expressed genes (DEGs) between two adjacent points and between vegetative mycelium and each point were detected by Tag Count Comparison ...RNA-seq data, expression levels of selected genes ... See full document

23

Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).

Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).

... (qRT-PCR) data, normalization relative to reliable reference gene(s) is ...stability of its reference genes have not been previously ...reference genes (RPL18, RPS3, AK, EF-1b, ... See full document

9

PRC1 and PRC2 are not required for targeting of H2A.Z to developmental genes in embryonic stem cells.

PRC1 and PRC2 are not required for targeting of H2A.Z to developmental genes in embryonic stem cells.

... gradient of increasing acetonitrile concentration, containing ...sec of MS followed by 3 sec MSMS analyses of the 2 most intense peaks seen by ...from analysis for the next 60 sec. MS/MS ... See full document

13

Characterization of five ECF sigma factors in the genome of Pseudomonas syringae pv. syringae B728a.

Characterization of five ECF sigma factors in the genome of Pseudomonas syringae pv. syringae B728a.

... factors of strain B728a were identified by ECF group and named according to the report’s ...expression of several virulence associated factors in ...members of the FecI-type of ECF sigma ... See full document

13

Identification of genes related to high royal jelly production in the honey bee( Apis mellifera ) using microarray analysis

Identification of genes related to high royal jelly production in the honey bee( Apis mellifera ) using microarray analysis

... Ten genes were randomly selected for confirmation using qRT-PCR to verify the accuracy of the identification of the DEG in the chip ...10 genes were signifi- cantly up-regulated ... See full document

9

Characterization of the floral transcriptome of Moso bamboo (Phyllostachys edulis) at different flowering developmental stages by transcriptome sequencing and RNA-seq analysis.

Characterization of the floral transcriptome of Moso bamboo (Phyllostachys edulis) at different flowering developmental stages by transcriptome sequencing and RNA-seq analysis.

... validity of Illumina analysis and assess the expression profiles in terms of specific mRNA abundances, 8 putative genes were selected and detected by qRT-PCR, and to further ... See full document

12

Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut

Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut

... reference genes were designed for peanut and analyzed by GeNorm and NormFinder ...endogenous control genes have been identified for other Arachis species, including the wild relatives which ... See full document

11

Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.

Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.

... Quantification of gene expression modulation is usually carried out by means of quantita- tive real-time reverse transcription-coupled PCR (qRT-PCR), and amplification data are ... See full document

22

A combined proteomic and transcriptomic analysis on sulfur metabolism pathways of Arabidopsis thaliana under simulated acid rain.

A combined proteomic and transcriptomic analysis on sulfur metabolism pathways of Arabidopsis thaliana under simulated acid rain.

... transcriptomic analysis, we performed quantitative real-time PCR (qRT-PCR) analysis on 12 genes, all of which are very crucial in S metabolism pathways, including S uptake ... See full document

11

Method for measuring factors that affect the performance of pilots

Method for measuring factors that affect the performance of pilots

... types of organizational factors that directly contribute to aeronautical acci- ...number of studies in the literature about the role that the error of the flight crew plays in the etiology of ... See full document

14

Micromass cultures are effective for differentiation of human amniotic fluid stem cells into chondrocytes

Micromass cultures are effective for differentiation of human amniotic fluid stem cells into chondrocytes

... differentiation of MSCs derived from UCB into chondrocytes (9) than other growth factors (TGF-b3 was combined with dexamethasone to induce ...expression of type II collagen, aggrecan and ...expression ... See full document

8

TLR3 Agonist Poly-IC Induces IL-33 and Promotes Myelin Repair.

TLR3 Agonist Poly-IC Induces IL-33 and Promotes Myelin Repair.

... expression of myelin is not ...release of IL-33 is caused by either cell death or ...absence of cell death is by activation of purinergic signaling pathways ...source of energy, it also ... See full document

24

Expressão gênica diferencial em animais cruzados Gir x Holandês infestados com o carrapato Rhipicephalus microplus

Expressão gênica diferencial em animais cruzados Gir x Holandês infestados com o carrapato Rhipicephalus microplus

... de genes. Dentre os genes mais expressos nos animais susceptíveis, podem ser destacados os genes codificantes de: indoleamina 2,3- deoxigenase 1, de CXCL10 e da proteina quinase associada a cadeia ... See full document

117

MicroRNA Expression Profiling in Clear Cell Renal Cell Carcinoma: Identification and Functional Validation of Key miRNAs.

MicroRNA Expression Profiling in Clear Cell Renal Cell Carcinoma: Identification and Functional Validation of Key miRNAs.

... target genes are directly regulated by miR-199a-5p, 3’ UTR segments of the corresponding genes containing the miR-199a-5p recognition region mutated region not recognizable by miR-199a-5p were ... See full document

16

Molecular cloning and expression analysis of fushi tarazu factor 1 in the brain of air-breathing catfish, Clarias gariepinus.

Molecular cloning and expression analysis of fushi tarazu factor 1 in the brain of air-breathing catfish, Clarias gariepinus.

... guidelines of Institutional Animal Ethical Committee (IAEC), however approval is not required for edible fish ...tissues of 3-4 larvae for each sample were used for each time point of ontogeny ... See full document

9

Identifying Stable Reference Genes for qRT-PCR Normalisation in Gene Expression Studies of Narrow-Leafed Lupin (Lupinus angustifolius L.).

Identifying Stable Reference Genes for qRT-PCR Normalisation in Gene Expression Studies of Narrow-Leafed Lupin (Lupinus angustifolius L.).

... purpose of this study is to provide an independent recommendation of suitable refer- ence genes for normalisation in qRT-PCR analyses of narrow-leafed ...housekeeping ... See full document

18

Caracterização molecular e análise filogenética de rotavírus a em população infantil com e sem diarreia no semiárido brasileiro.

Caracterização molecular e análise filogenética de rotavírus a em população infantil com e sem diarreia no semiárido brasileiro.

... A metodologia do Luminex consiste basicamente na técnica de reação em cadeia da polimerase (PCR) associada à hibridização com microesferas de poliestireno acopladas a sondas específicas para cada alvo. As ... See full document

93

Bayesian factor analysis for mixed data on management studies

Bayesian factor analysis for mixed data on management studies

... assignment of numerals to objects or events according to ...scales of measurement that are quite different and cannot be used interchangeably even though they may be represented by ...number of ... See full document

16

Prognostic significance of miR-181b and miR-21 in gastric cancer patients treated with S-1/Oxaliplatin or Doxifluridine/Oxaliplatin.

Prognostic significance of miR-181b and miR-21 in gastric cancer patients treated with S-1/Oxaliplatin or Doxifluridine/Oxaliplatin.

... amount of efforts have been focused on discover- ing predictive and prognostic biomarkers for gastric cancer ...complicity of gastric cancer, it still remains a major challenge to identify clinical useful ... See full document

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