[PDF] Top 20 Detection of Dehalococcoides spp. by peptide nucleic acid fluorescent in situ hybridization
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Detection of Dehalococcoides spp. by peptide nucleic acid fluorescent in situ hybridization
... concentration in each field of view in the ...ratio by increasing the number of cells in the field of view relative to the occasional debris that might be present on the ... See full document
13
Optimization of peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria: The effect of pH, dextran sulfate and probe concentration
... optimum of 300 nM). The fluorescence values (in arbitrary units) of the contour lines are the ones obtained for ...predicted by the software for ...represented in black circles with its ... See full document
35
Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids
... Different hybridization conditions were used, depending on the nucleic acid composition, length, and chemical nature of each ...range of 25–70 °C in pure cultures of ... See full document
16
Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of helicobacter pylori clarithromycin resistance in gastric biopsy specimens
... established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection ... See full document
8
Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia
... Study of karyotype was done using peripheral blood whose buffy coat was divided and placed in 2 culture flasks with 8 ml RPMI 1640 medium, pH ...followed by hypotonic treatment with ...acetic ... See full document
4
Optimizing locked nucleic acid/2′-O-methyl-RNA fluorescence in situ hybridization (LNA/2′OMe-FISH) procedure for bacterial detection
... application of nucleic acid mimics (NAMs) have emerged for in vivo and in vitro applications in the health area ...]). In particular, locked nucleic acid ... See full document
16
Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens
... shown in Table 2, Hpy769 hybridizes with all ...no hybridization was observed for the other bacterial species ...used in this study. Positive detection of culture collections was ... See full document
6
A new colorimetric peptide nucleic acid-based assay for the specific detection of bacteria
... Several hybridization procedures using PNA probes have been described in recent years, but most of them are based on fluorescently-labeled probes ...application of this technique to ... See full document
16
Optimization of a peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the detection of bacteria and disclosure of a formamide effect
... identified by 16S rRNA gene sequencing (Simões et ...Cells in the exponential growth phase were harvested from plates, suspended in sterile water and homogenized by vortexing in an ... See full document
21
Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
... Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely- used tech- nique in ...step of FISH—fixation/permeabilization—is crucial to the outcome of the ... See full document
13
Conjugates of Peptide Nucleic Acid-Biotin for the Detection of Bacteria using in Situ Hybridization
... presence of biotin-streptavidin binding, the most popular method uses enzymes (which are conjugated with streptavidin) capable of generating a colour change when they are incubated in the presence ... See full document
53
Discrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH)
... ability of this technique to distinguish the phage- infected population within complex structures (Figure ...embedded in paraffin and cut into transverse ...μm in diameter, are visible in the ... See full document
17
A modern mode of activation for nucleic acid enzymes.
... mode of activation to other catalytic nucleic ...All of these nucleic acid enzymes share a relatively similar ...site of the substrates is located in the middle of ... See full document
7
Tetrasomy 15Q11-Q13 identified by fluorescence in situ hybridization in a patient with autistic disorder
... b in ação d e m et o d o lo g ias d a cit o g en ét ica clássica e m o lecu lar p ela t écn ica d e h i b r i d ação in situ f l u o r escen t e, d em o n st r o u o car i ó t i p o co m o 4 7 , XX, ... See full document
5
Detection of cell carcinogenic transformation by a quadruplex DNA binding fluorescent probe.
... for in vitro analysis ...density of 10 4 cells/60 mm dish and then incubated for 24 ...doses of 3-methyl-cholanthrene (MCA, Sigma-Aldrich, ...maintained in culture for 2–5 weeks, with medium ... See full document
10
Digital microfluidics for nucleic acid amplification
... Nucleic acid amplification is one of the most relevant tools in molecular biology, allowing the monitorization of gene expression, quantification of food-borne pathogens, ... See full document
13
Chromosomal imbalances detected in primary bone tumors by comparative genomic hybridization and interphase fluorescence in situ hybridization
... gain of 8q23 sequences, without involvement of the CMYC oncogene, as verified by FISH (Stock et ...overrepresentation of the EBAG9 gene, CMYC, or both, in 58/129 breast tu- ... See full document
7
Analysis of endodontic pathogens by classical microbiological and nucleic acid approaches
... combination of Culture and Molecular identification approaches, have confirmed the polymicrobial nature of primary endodontic infections with a predominance of anaerobic ...component of the ... See full document
277
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
... panel of 156 DNAs ob- tained from ...mutations in the hot-spot region of the rpoB and associated with RIF-resistance in this region (Table ...collection of the Institute of ... See full document
7
Cytogenetic study of Anopheles albitarsis (Diptera: Culicidae) by C-banding and in situ hybridization
... variation in the size of the rDNA cistrons of ...difference in the distribution of rDNA copies asso- ciated with constitutive ...structure of A. albitarsis sensu lato and ... See full document
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