[PDF] Top 20 Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil

... aim of this study was to detect the IS900 region of Mycobacterium avium ...(MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and ... See full document

... provide the sanitary status of the herd, and also inform the health condition of the owners, the aim of this study was to verify the presence of MAP ... See full document

... for Mycobacterium avium subspecies paratuberculosis (MAP) isolation are based on the MAP detection from feces and not ...milk. The choice of the best ... See full document

... persist in unfavorable conditions such as those encountered in soil and aquatic ...like state to survive the pressures applied by pasteurization and thereby provide one explanation for MAP ... See full document

... investigation of Mycobacterium avium subsp. paratuberculosis (MAP) infection in dairy cattle of the Garanhuns microre- gion, in Pernambuco, ... See full document

... Mycobacterium avium subsp. paratuberculosis is known as the etiologic agent of Johne’s disease, or paratuberculosis, which affects mostly ...M. paratuberculosis ... See full document

... isolated Mycobacterium sp. organisms from several patients with ...revived the idea of a mycobacterial cause for this human disease (Chiodini, Van Kruiningen, Merkal, Thayer & Coutu, ... See full document

... aim of this survey was to identify spatial clustering of bovine paratuberculosis po- sitive herds in the State of Paraíba, Northeastern ...Brazil. The ... See full document

... ranges from 5 to 25% (Martínez-Covarrubias et al., 2012). In Mexico, PTB is widely distributed, with studies indicating that the rate of prevalence is between 5 and 30%, primarily in ... See full document

... reported the different methods used for detection of MAP in milk and milk products and analyzed the current methods for direct or non-direct identification of MAP ... See full document

... 20ng/μL of DNA. Then, 20ng of positive control were added to the diluted samples and qPCR was ...had the same Cq in the qPCR; therefore, there were no inhibitors in ... See full document

... evidence of dog attack lesions from a dairy farm with a history of bovine paratuberculosis, where fecal culture prevalence was estimated to be 10% in a survey previously carried ... See full document

... explained in Tables 2 and 3, significant differences were observed in the frequency of positive animals between the cows with and without clinical ...seen in the number ... See full document

... enhance the risk of severe infections in humans, mice, and domestic cattle, including the potential for increased susceptibility to Johne’s disease, a debilitating and economically important ... See full document

... sample of milk, a loopful of MAP positive control was inoculated in 50 mL of milk powder Molico® (Nestlé, São Paulo, Brazil) reconstituted with distilled water and ... See full document

... assessments of odds ratio and RR in studies of para- tuberculosis is usually related to the search for risk factors associated with management practices applied to livestock and the ... See full document

... tracing the sources of infec- tion and for studying the epidemiology, population structure, and evolutionary relationships between ...reveal the genetic diversity underlying important ... See full document

... A similaridade da DC em humanos com a DJ em bovinos revelada por dados histopatológicos (inflamação entérica crónica, presença de granulomas, infiltrados inflamatórios e espessamentos) é, pelo menos, sugestivoa de uma ... See full document

... throughout the lamina propria and mucosa, as previously reported (Perez et ...by the presence of more than one precipitin line indicating a strong positive reac- ...on the LJ media with ... See full document

... failure in the reproductive system of goats and sheep. The species that are most commonly affected are ...2013). The pre- sence of these microorganisms in the ... See full document