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[PDF] Top 20 Dissecting mitosis by RNAi in Drosophila tissue culture cells

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Dissecting mitosis by RNAi in Drosophila tissue culture cells

Dissecting mitosis by RNAi in Drosophila tissue culture cells

... analysis in Drosophila remains one of the most powerful methods to determine the function of a particular protein involved in cell division (reviewed in ...alleles. In those cases, ... See full document

9

A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.

A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.

... genome-wide RNAi screen in Drosophila tissue culture cells (S2Rþ) designed to identify proteins required for centriole duplication and centrosome ...implicated in ... See full document

16

A genome-wide RNAi screen identifies regulators of cholesterol-modified hedgehog secretion in Drosophila.

A genome-wide RNAi screen identifies regulators of cholesterol-modified hedgehog secretion in Drosophila.

... genes in protein/membrane traffic, we examined the effect of the depletion of the four candidate genes on ER-Golgi trafficking and Golgi structure using immunofluorescence and electron micros- copy ...retained ... See full document

15

Assembly of Drosophila centromeric chromatin proteins during mitosis.

Assembly of Drosophila centromeric chromatin proteins during mitosis.

... S2 cells stably expressing SNAP-tagged CID, CAL1 and CENP-C and GFP-tubulin or clonal Kc167 cells expressing SNAP-CID were diluted at 1610 6 cells/ml in serum medium two days prior to ... See full document

17

Organotypic tissue culture of adult rodent retina followed by particle-mediated acute gene transfer in vitro.

Organotypic tissue culture of adult rodent retina followed by particle-mediated acute gene transfer in vitro.

... after culture, probably because of loss of retinal pigmental epithelium and shorter outer segments of ...ganglion cells after 4-day culture were able to generate action potential by ... See full document

9

Expression in aneuploid Drosophila S2 cells.

Expression in aneuploid Drosophila S2 cells.

... S2 cells were fixed with 1% formaldehyde in tissue culture media for 10 min at room ...PBS, cells were collected and resuspended in cell lysis buffer (5 mM PH ...resuspended ... See full document

12

Dissecting systemic RNA interference in the red flour beetle Tribolium castaneum: parameters affecting the efficiency of RNAi.

Dissecting systemic RNA interference in the red flour beetle Tribolium castaneum: parameters affecting the efficiency of RNAi.

... For RNAi experiments, the length of dsRNA used varies widely depending on the context and model organism (generally ranging from 21 to 1,000 base ...pairs). In plants, dsRNA length does not cause much ... See full document

14

Mutations in Drosophila Greatwall/Scant reveal its roles in mitosis and meiosis and interdependence with Polo kinase.

Mutations in Drosophila Greatwall/Scant reveal its roles in mitosis and meiosis and interdependence with Polo kinase.

... behavior in the more conventional cell cycles of the larval central nervous ...delay in late G2 to anaphase in gwl mutant neuroblasts in addition to chromatin condensa- tion defects [13] and ... See full document

17

Phytoceramide in vertebrate tissues: one step chromatography separation for molecular characterization of ceramide species.

Phytoceramide in vertebrate tissues: one step chromatography separation for molecular characterization of ceramide species.

... sphingolipids in vertebrates, while plants contain phytoceramide. By using a novel chromatography purification method we show that phytoceramide comprises a significant proportion of animal ...mouse ... See full document

8

INDUCED PLEURIPOTENT STEM CELLS: A PIONEER IN THERAPY

INDUCED PLEURIPOTENT STEM CELLS: A PIONEER IN THERAPY

... stem cells can potentially be used to counter a wide range of diseases, from diabetes to spinal cord injury, to childhood leukemia, to heart ...iPS cells are similar to human embryonic stem (ES) ... See full document

14

Identification and characterization of a stage specific membrane protein involved in flagellar attachment in Trypanosoma brucei.

Identification and characterization of a stage specific membrane protein involved in flagellar attachment in Trypanosoma brucei.

... approved by the Bio Resources Ethics Review committee at Trinity College Dublin and was performed under a certificate A licence issued by the Minister for Health and Children under the Cruelty to Animals ... See full document

12

Alteration of cellular behavior and response to PI3K pathway inhibition by culture in 3D collagen gels.

Alteration of cellular behavior and response to PI3K pathway inhibition by culture in 3D collagen gels.

... role in drug ...3D culture techniques is not enough to ensure a more robust and predictive ...concentration in our gels was sufficient to drastically alter cell behavior in one cell line, and ... See full document

11

ActRIIA and BMPRII Type II BMP receptor subunits selectively required for Smad4-independent BMP7-evoked chemotaxis.

ActRIIA and BMPRII Type II BMP receptor subunits selectively required for Smad4-independent BMP7-evoked chemotaxis.

... For RNAi-mediated down-regulation of type II BMP receptor subunits, synthetic oligonucleotides were designed to target sequences 100% conserved in human, mouse and rat ActRIIA, BMPRII and Smad4, using shRNA ... See full document

14

BLG ) gene by lentivirus-mediated RNAi

BLG ) gene by lentivirus-mediated RNAi

... used in innumerous cases of gene silencing. Through induction by plasmids or virus vectors, RNAi has broadened its range of application (Bartel, ...animal cells, to so achieve continuous long- ... See full document

6

Mesenchymal Stem Cells - Emphasis in Adipose Tissue

Mesenchymal Stem Cells - Emphasis in Adipose Tissue

... stem cells has evolved rapidly in recent ...these cells are potentially able to become any cell type and have the power of self-renewal throughout the life of the ...stem cells (MSCs) can be ... See full document

12

Efeito terapêutico de células-tronco mesenquimais no tratamento da sepse experimental

Efeito terapêutico de células-tronco mesenquimais no tratamento da sepse experimental

... characterized by a generalized inflammatory state caused by ...made in developing new treatments and mortality rates are virtually the same in the last 20 to 30 ...stem cells have ... See full document

87

Gallus gallus domesticus: immune system and its potential for generationof immunobiologics

Gallus gallus domesticus: immune system and its potential for generationof immunobiologics

... IgY antibody shares structural similarities with mammalian IgG, like the antigen binding fragment (fab) with complementarity determining regions (CdR) and crystallizable fragments (fc). However, IgY antibody lacks a ... See full document

8

Road March Searching a Better Alternative Way of Hartal Culture in Bangladesh

Road March Searching a Better Alternative Way of Hartal Culture in Bangladesh

... work in the factories which causes nations loss as productions cannot reach up to mark (Islam Z, ...find in the newspapers the pictures of wanton acts of vandalism like destruction of government and private ... See full document

8

In vitro osteoclastogenesis on textile chitosan scaffold

In vitro osteoclastogenesis on textile chitosan scaffold

... the cells to the given ...the cells and the uncoated ...osteoclast-like cells have formed and are attached along the chitosan fibres after 12 days of ...skeleton. In case of collagen coating ... See full document

11

The human adenovirus type 5 E1B 55 kDa protein obstructs inhibition of viral replication by type I interferon in normal human cells.

The human adenovirus type 5 E1B 55 kDa protein obstructs inhibition of viral replication by type I interferon in normal human cells.

... accumulate in infected cell nuclei [111], this activity could contribute to repression of expression of interferon-sensitive genes by the E1B 55 kDa protein ...export in infected cells depends ... See full document

15

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