[PDF] Top 20 DNA extraction, amplification and sequencing
Has 10000 "DNA extraction, amplification and sequencing" found on our website. Below are the top 20 most common "DNA extraction, amplification and sequencing".
DNA extraction, amplification and sequencing
... L-INS–i and G-INS-i criteria (for nrITS and nrLSU, ...ITS and 841 bp for ...ITS and 28S to determine whether there were any well-supported phylogenetic incongruencies between these two ... See full document
10
Clonal multidrug-resistant Corynebacterium striatum within a nosocomial environment, Rio de Janeiro, Brazil
... Gene amplification and sequencing - ...rRNA and rpoB gene ...30ºC and centrifuged for 5 min at 3,000 ...water and subsequently boiled for 15 min for DNA ... See full document
7
PCR AMPLIFICATION AND SEQUENCING
... DNA extraction was performed from a similar protocol to that proposed by Castalanelli et ...solution and then heating for a period not exceeding 30 minutes at temperatures between 70°C and ... See full document
7
Genetic materials and DNA extraction
... (2000) and Rovelli et ...of DNA, 0.6 unit of Taq DNA polymerase, buffer 1x, 1mM of MgCl 2 , 150 µM of each dNTP and ...The amplification was done using the procedure of touchdown-PCR ... See full document
7
Tuberculosis Biomarker Extraction and Isothermal Amplification in an Integrated Diagnostic Device.
... automated DNA extraction and amplification, chemical lysis was performed on surrogate sputum samples provided by ...buffers, and ampli- fication reaction solution as described above ... See full document
14
Culture-Independent Identification of Nontuberculous Mycobacteria in Cystic Fibrosis Respiratory Samples.
... CF and a history of NTM infection were identified with ...sputum and four BAL) were identified in our collec- tion that were NTM culture-positive (seven MABSC and eight ...consistency and ... See full document
13
Bacterial isolates and DNA extraction
... variability and symbiotic efficiency of Papilionoidae rhizobial communities are very ...variability and the symbiotic efficiency of eight rhizobial isolates obtained from “mulungu” (Erythrina velutina ... See full document
13
Digital Droplet Multiple Displacement Amplification (ddMDA) for Whole Genome Sequencing of Limited DNA Samples.
... Green and 0.15 μM of ϕ29 DNA polymerase (New England Biolabs, Ipswich, ...MDA and transferred to a 200 μL PCR tube, while the rest 280 μL was used for droplet ...template and MDA mix were ... See full document
12
Evaluation of methods for the extraction and purification of DNA from the human microbiome.
... six DNA extraction methods previously used in studies of the human microbiome and environmental samples to recover DNA from known organisms and yield genomic DNA representative ... See full document
10
PCR-TTGE analysis of 16S rRNA from rainbow trout (Oncorhynchus mykiss) gut microbiota reveals host-specific communities of active bacteria.
... F2 and F3 were clustered together (Figure 2A and 2B) and shared some bacterial components (Figure ...F2 and F3 clustered Figure ...the amplification of the V3–V4 region of the 16 S rRNA ... See full document
10
MATERIALS AND METHODS Bacterial strains, growth conditions and DNA extraction
... Lisitsyn and coworkers (33) and the modification of the RDA procedure described by Tinsley and Nassif ...tester and driver DNAs are digested to completion with BglII and complementary ... See full document
10
Sequencing intractable DNA to close microbial genomes.
... 5 and 6 (Table 1) yielded sequences within the gap, however, internal stoppage likely prevented read-through despite the use of ...5, and 6. The estimated size of gaps 5 and 6 were 65 and 35 ... See full document
9
Streamlining DNA barcoding protocols: automated DNA extraction and a new cox1 primer in arachnid systematics.
... [1–8] and the same gene is also among the usual markers employed in phylogenetic, genetic and genomic analyses ...spiders and other arachnids, the standard barcoding region — 650 base pair long ... See full document
12
Rapid Yeast DNA Extraction By Boiling And Freeze- Thawing Without Using Chemical Reagents And DNA Purification
... the DNA which was re-suspended in sterile distilled water and heated to 95 °C for 3 ...for DNA-extraction used by de Baere et ...water and heated at 95°C for 15 min and submitted ... See full document
10
2) Antimicrobial susceptibility testing and DNA extraction of isolated strains
... chloramphenicol and beta-lactams resistance genes (sul1, cat1 and SHV) was performed in a total volume of 25 μl reaction containing 10ng of DNA extracted from each isolated strain, with final ... See full document
6
Comparison of protocols for the extraction of genomic DNA from Sharpshooters (Hemiptera: Cicadellidae) for the detection of Xylella fastidiosa
... cases, DNA extrac‑ tion is not efficient and several other molecules can be extracted alongside the DNA, which can inhibit the PCR reaction, gener‑ ating a false‑negative ...Xylella, and 11 ... See full document
5
Analysis of high-throughput sequencing and annotation strategies for phage genomes.
... large-scale amplification of the linker- ligated, size-fractionated DNA fragments, a PCR titration experiment was performed to determine the lowest possible number of amplification rounds suitable to ... See full document
12
DNA extraction and amplification conditions
... ancient and were derived from different processes, such as differentiation from wild grapevines, spontaneous crosses between wild grapevines and cultivars or crosses between ...juices and wine, with ... See full document
10
Amplification, cloning and DNA sequencing of the
... resistance, and absence of effective ...effective and higher therapeutic value drugs are ...enzymes and thus we selected this enzyme as the potential therapeutic ...mutagenesis, and cloning of ... See full document
6
DNA extraction, PCR, and DNA sequencing
... species and the large number of common hosts (PARRELLA et al., 1982). Genetic and biological studies done in other populations of Liriomyza ...the DNA barcoding has enormous potential for ... See full document
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