[PDF] Top 20 DNA extraction, PCR, and DNA sequencing
Has 10000 "DNA extraction, PCR, and DNA sequencing" found on our website. Below are the top 20 most common "DNA extraction, PCR, and DNA sequencing".
DNA extraction, PCR, and DNA sequencing
... distributed and have economic importance as they cause damage to at least 14 plant families, especially Solanaceae, Cucurbitaceae, Asteraceae, and ...Blanchard and L. huidobrensis (Blanchard). The ... See full document
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COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES
... available dNa extraction kits (Wizard® Genomic dNa Purification Kit, High Pure PCr Template Kit, dNeasy mericon Food and GenejeT PCr Purification Kit), as well as standard ... See full document
5
Genetic Structure of Czech and Slovak Populations of the Eurasian Otter (Lutra lutra): DNA Typing of Spraints and Microsatellite Variability
... Stool DNA Mini Kit (Qiagen,) or Invisorb Spin Stool DNA Kit (Invitek), following manufacturer’s instructions with only minor ...Extracted DNA was used as a template in Polymerase Chain Reactions ... See full document
7
Comparison of DNA extraction using proteinase K and extraction kit: analysis of the quality of the genetic material
... do DNA foi verificada em gel de agarose a 2%, com brometo de ...A PCR amplificou o gene Large no DNA extraído, independente da metodologia ...do DNA é ... See full document
6
Sample collection and DNA extraction
... Resumo – Os objetivos deste trabalho foram identificar a origem racial de ovinos Santa Inês e avaliar a unicidade das populações das principais raças naturalizadas brasileiras de ovinos deslanado. Foi realizado um estudo ... See full document
7
Evaluation of five methods for total DNA extraction from western corn rootworm beetles.
... virgifera, DNA extractions by the SDS method, CTAB method, DNAzolH reagent, PuregeneH solutions and DNeasyH column were compared in terms of DNA quantity and quality, cost of materials, ... See full document
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Genetic materials and DNA extraction
... (2000) and Rovelli et al. (2000) were analyzed. The PCR reaction was realized in a total volume of 20 µL containing 50 ηg of DNA, ...Taq DNA polymerase, buffer 1x, 1mM of MgCl 2 , 150 µM of ... See full document
7
Evaluation of methods for the extraction and purification of DNA from the human microbiome.
... six DNA extraction methods previously used in studies of the human microbiome and environmental samples to recover DNA from known organisms and yield genomic DNA representative ... See full document
10
Unveiling distribution patterns of freshwater phytoplankton by a next generation sequencing based approach.
... NGS and morphological based data cannot be avoided (Figure ...the DNA extraction and PCR procedures ...optimize and standardize all steps for an objective assessment of true ... See full document
10
Amplificação da sequência de inserção IS6110 diretamente do esfregaço das lâminas coradas para bacilo álcool- ácido resistente
... various DNA extraction methods and found an efficiency variation between the methods of 75% to 90%, which proves that the extraction method directly influences the IS6110-PCR ...steps ... See full document
60
Streamlining DNA barcoding protocols: automated DNA extraction and a new cox1 primer in arachnid systematics.
... automated DNA extraction protocol. Compared with manual extraction procedures using kits, robotic DNA extraction methods often yield lower quantity of extracted DNA ...robotic ... See full document
12
An Efficient and Rapid DNA Minipreparation Procedure Suitable for PCRSSR and RAPD Analyses in Tropical Forest Tree Species
... extracted DNA was not degraded and contained no RNA contamination ...the DNA samples ranged from ...This DNA extraction method, excluding the Proteinase K, was also tested with frozen ... See full document
8
Bacterial isolates and DNA extraction
... legumes, and advances in the taxonomy of their rhizobia have shown the prevalence of β-rhizobia, mainly Paraburkholderia ...isolate and classify the rhizobial community from Papilionoidae trees from the ... See full document
13
Rapid Yeast DNA Extraction By Boiling And Freeze- Thawing Without Using Chemical Reagents And DNA Purification
... the DNA which was re-suspended in sterile distilled water and heated to 95 °C for 3 ...for DNA-extraction used by de Baere et ...water and heated at 95°C for 15 min and submitted ... See full document
10
MATERIALS AND METHODS Bacterial strains, growth conditions and DNA extraction
... Lisitsyn and coworkers (33) and the modification of the RDA procedure described by Tinsley and Nassif ...tester and driver DNAs are digested to completion with BglII and complementary ... See full document
10
PCR AMPLIFICATION AND SEQUENCING
... DNA extraction was performed from a similar protocol to that proposed by Castalanelli et ...solution and then heating for a period not exceeding 30 minutes at temperatures between 70°C and ... See full document
7
Comparison of protocols for the extraction of genomic DNA from Sharpshooters (Hemiptera: Cicadellidae) for the detection of Xylella fastidiosa
... cases, DNA extrac‑ tion is not efficient and several other molecules can be extracted alongside the DNA, which can inhibit the PCR reaction, gener‑ ating a false‑negative ...Xylella, ... See full document
5
DNA sequencing analysis of several G6PD variants previously defined by PCR-restriction enzyme analysis
... site and shows a complex elec- trophoretic banding pattern (Arámbula, ...mutation and the silent polymorphisms at nt 381 C ® T and nt 384 C ® T (Beutler and Vulliamy 2002; Hamel et ... See full document
5
Extraction of viral DNA PCR qPCR: The viral genomes present
... controls and samples were run in duplicates; the limit of detection was found to be 40 to 60 genome copies per reaction, the efficiency was ...(NTC), and AdV negative samples were used in each run to ensure ... See full document
6
Extraction and quantification of DNA
... The PCR-RFLP was conducted to confirm the results of multiplex ...The PCR reaction of each gene was performed with their universal primers (Table ...The PCR products were digested with restriction ... See full document
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