Top PDF DNA methylation dynamics in human induced pluripotent stem cells over time.
DNA methylation dynamics in human induced pluripotent stem cells over time.
... methylated stem cell-specific DMRs and significantly higher expression, and 17 genes with the hyper-methylated stem cell- specific DMRs and significantly lower expression in iPSCs/ESCs from 22 iPS ...
14
Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.
... first time the number and characteristics of the acquired mutations in a monoclonal cell isolated from a healthy individual and subsequently derived ...iPSCs in long-term culture, demonstrating how ...
15
The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture.
... resulted in the most homogenous hiPSC population with the lowest load of aberrant DNA methylations in analysed ...transgenes in ontogenetically younger NDF source ...types. In contrast, ...
16
Scalable passaging of adherent human pluripotent stem cells.
... Advancing pluripotent stem cell research to clinical applications requires adapting laboratory-scale cultivation methods to less conventional and current Good Manufacturing Practices- (cGMP- ) compliant ...
9
Evaluation of the cardiotoxicity of mitragynine and its analogues using human induced pluripotent stem cell-derived cardiomyocytes.
... with human ventricular tissue as well as with hiPSC-CMs by ...the cells treated with Mitragynine for 24 hours was examined by Trypan Blue exclusion assay and over 85% viability was ...observed. ...
18
Few single nucleotide variations in exomes of human cord blood induced pluripotent stem cells.
... To validate SNVs identified by bioinformatic analysis, we used a real-time PCR approach. We designed 3 primers for each point mutation. Two forward primers, with SNV site at the 3 9 end, were manually designed and ...
9
Generating a non-integrating human induced pluripotent stem cell bank from urine-derived cells.
... (listed in Table 1). The generation of iPS cells from UCs listed in Table 2 is ...illustrated in Figure ...deposited in the ...endogenous pluripotent genes such as OCT4, SOX2 and ...
7
Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells
... up in three biological replications with the Power SYBR GreenMaster Mix (Applied Biosys- tems, USA) and analyzed with the 7500 real-time (RT) PCR system (Applied Biosystems, ...presented in table ...
14
Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells.
... from induced pluripotent stem cells (iPSCs) without contamination from unknown substances is crucial to their use in clinical ...progenitor cells, which retain hematopoietic and ...
10
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats
... resuspended in Dulbecco’s Modiied Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F12) (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) (Cultilab, Campinas-SP, Brazil), 100 U/ml penicillin (Sigma- ...
5
Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.
... of human induced pluripo- tent stem ...Primary human keratinocytes (PHK) derived from skin biopsy were reprogrammed to iPSC with a modified version of our ‘hit and run’ vector and subsequently ...
10
Induced pluripotent stem cells, a giant leap for mankind therapeutic applications
... of human induced pluripotent stem ...on cells differentiated from patient-derived induced pluripotent stem cells (iPSC) to model the disease (blue ...
14
Molecular insights into reprogramming-initiation events mediated by the OSKM gene regulatory network.
... [54]. In the next step, GSK3 inhibition exhibits multiple ...differentiation in ES cells and promote reprogramming of somatic ...of human ES cells in an undifferentiated state ...
17
Physical passaging of embryoid bodies generated from human pluripotent stem cells.
... phase cells (15.68%62.33) in 7 d-hEBs ...differences in the cell-cycle distribution relative to 7 d- hEBs, however, 5 w-hEBs demonstrated a reduced number of cells in S phase ...of ...
9
Changes in liver cell DNA methylation status in diabetic mice affect its FT-IR characteristics.
... University of Campinas. The animals were reared under standard controlled conditions, fed extruded chow (Nuvital, Colombo, Brazil) and received water ad libitum. Their glycemia levels were measured through blood samples ...
7
Pluripotent transcription factors possess distinct roles in normal versus transformed human stem cells.
... reduction in Oct4 and Nanog levels induces spontaneous lineage development and loss of pluripotent self-renewal capacity in hPSCs ...transformed cells (Figure 4L). In contrast, Nanog ...
9
Derivation, characterization, and stable transfection of induced pluripotent stem cells from Fischer344 rats.
... the time course of our studies another group reported a riPS cell derivation protocol that used both REFs and rat neural precursors as starting cell types and relied on serum-containing medium, non- excisable ...
9
Arq. NeuroPsiquiatr. vol.73 número11
... and in the search for therapeutic strategies for this ...heteroplasmy, cells carrying both normal and mutant mtDNA, is a typical feature of pathogenic mtDNA mutations, that contributes to the clinical ...
2
Culture conditions affect cardiac differentiation potential of human pluripotent stem cells.
... positive cells could be detected in long-term culture of hPSCs on Matrigel matrix together with mTeSR1 medium than in MEF and SNL feeder cell ...co-culture in cells cultured on Matrigel ...
13
Differentiation of human pluripotent stem cells to the neuronal dopaminergic fate
... PA6 cells as feeder layer by Zeng et al (Zeng, Cai, & Chen, ...cultured in Glasgow Minimum Essential Media (GMEM) for 3 weeks, hESc formed colonies, approximately 70% of which were positive for TuJ1; ...
42
