[PDF] Top 20 DNA methylation dynamics in human induced pluripotent stem cells over time.

... methylated stem cell-specific DMRs and significantly higher expression, and 17 genes with the hyper-methylated stem cell- specific DMRs and significantly lower expression in iPSCs/ESCs from 22 iPS ... See full document

... first time the number and characteristics of the acquired mutations in a monoclonal cell isolated from a healthy individual and subsequently derived ...iPSCs in long-term culture, demonstrating how ... See full document

... resulted in the most homogenous hiPSC population with the lowest load of aberrant DNA methylations in analysed ...transgenes in ontogenetically younger NDF source ...types. In contrast, ... See full document

... Advancing pluripotent stem cell research to clinical applications requires adapting laboratory-scale cultivation methods to less conventional and current Good Manufacturing Practices- (cGMP- ) compliant ... See full document

... with human ventricular tissue as well as with hiPSC-CMs by ...the cells treated with Mitragynine for 24 hours was examined by Trypan Blue exclusion assay and over 85% viability was ...observed. ... See full document

... To validate SNVs identified by bioinformatic analysis, we used a real-time PCR approach. We designed 3 primers for each point mutation. Two forward primers, with SNV site at the 3 9 end, were manually designed and ... See full document

... (listed in Table 1). The generation of iPS cells from UCs listed in Table 2 is ...illustrated in Figure ...deposited in the ...endogenous pluripotent genes such as OCT4, SOX2 and ... See full document

... up in three biological replications with the Power SYBR GreenMaster Mix (Applied Biosys- tems, USA) and analyzed with the 7500 real-time (RT) PCR system (Applied Biosystems, ...presented in table ... See full document

... from induced pluripotent stem cells (iPSCs) without contamination from unknown substances is crucial to their use in clinical ...progenitor cells, which retain hematopoietic and ... See full document

... resuspended in Dulbecco’s Modiied Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F12) (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) (Cultilab, Campinas-SP, Brazil), 100 U/ml penicillin (Sigma- ... See full document

... of human induced pluripo- tent stem ...Primary human keratinocytes (PHK) derived from skin biopsy were reprogrammed to iPSC with a modified version of our ‘hit and run’ vector and subsequently ... See full document

... of human induced pluripotent stem ...on cells differentiated from patient-derived induced pluripotent stem cells (iPSC) to model the disease (blue ... See full document

... [54]. In the next step, GSK3 inhibition exhibits multiple ...differentiation in ES cells and promote reprogramming of somatic ...of human ES cells in an undifferentiated state ... See full document

... phase cells (15.68%62.33) in 7 d-hEBs ...differences in the cell-cycle distribution relative to 7 d- hEBs, however, 5 w-hEBs demonstrated a reduced number of cells in S phase ...of ... See full document

... University of Campinas. The animals were reared under standard controlled conditions, fed extruded chow (Nuvital, Colombo, Brazil) and received water ad libitum. Their glycemia levels were measured through blood samples ... See full document

... reduction in Oct4 and Nanog levels induces spontaneous lineage development and loss of pluripotent self-renewal capacity in hPSCs ...transformed cells (Figure 4L). In contrast, Nanog ... See full document

... the time course of our studies another group reported a riPS cell derivation protocol that used both REFs and rat neural precursors as starting cell types and relied on serum-containing medium, non- excisable ... See full document

... and in the search for therapeutic strategies for this ...heteroplasmy, cells carrying both normal and mutant mtDNA, is a typical feature of pathogenic mtDNA mutations, that contributes to the clinical ... See full document

... positive cells could be detected in long-term culture of hPSCs on Matrigel matrix together with mTeSR1 medium than in MEF and SNL feeder cell ...co-culture in cells cultured on Matrigel ... See full document

... PA6 cells as feeder layer by Zeng et al (Zeng, Cai, & Chen, ...cultured in Glasgow Minimum Essential Media (GMEM) for 3 weeks, hESc formed colonies, approximately 70% of which were positive for TuJ1; ... See full document