[PDF] Top 20 Embryonic Stem-like Cells Derived from in vitro Produced Bovine Blastocysts

... of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced ...murine embryonic fibroblasts ...layer. ... See full document

... issue in bovine somatic cell nuclear transfer ...cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)–derived embryos ... See full document

... dehydrated in graded ethanols, cleared in xylene, and paraffin-embedded ...1% bovine serum albumin, and 0.1% Triton X-100 in phosphate-buffered saline, pH ...antibodies in blocking ... See full document

... embedded cells as described [59] with some modifications. Twenty cells (oocytes or granulosa cells from mouse ovary, and oocyte-like cells from FLSs) were embedded ... See full document

... of blastocysts. For this purpose, 2,355 bovine oocytes were divided into six experimental groups and analyzed in five replicates (314-352 oocytes per ...the blastocysts produced ... See full document

... Neural stem (NS) cell lines have previously been established from human fetal cortex (embryonic day 50–55) ...NS cells resemble radial glia and, although maintained in adherent culture ... See full document

... germ cells, and then, the expression during male and female gametogenesis exhibits sex-specific down- regulation similar to SSEA1 ...identified in migrating germ cells through to the post-meiotic ... See full document

... Human embryonic and induced pluripotent stem cells (hESC/hiPSC) are promising cell sources for the derivation of large numbers of specific cell types for tissue engineering and cell therapy ... See full document

... GSC-like cells was not changed during in vitro differentiation ...GSC-like cells in the inner wells and testicular cells in the outer wells ...testicular ... See full document

... engrafted stem cells into the developing or adult central nervous systems are particularly important not only because they have been contributing to elucidate how these cells may interact with the ... See full document

... cultivated bovine luteal cells from three different thirds of pregnancy behave the same way as in vivo luteal cells relative to P4 ...samples from days 90 (n=3), 150 (n=3) and ... See full document

... investigate in detail the changes in H3K9me2-marked heterochro- matin domains during terminal differenti- ation, Lienert et ...robust in vitro neurogenesis system to differentiate ES ... See full document

... improvement in best-corrected visual acuity was measured in four patients one week after the injection and was maintained throughout the ...ABMCs in eyes with advanced RP or cone-rod dystrophy was ... See full document

... listed in Table 1. To form teratomas, approximately 2610 6 cells were injected into hind limb muscle of 5-week-old nude ...fixed in 4% paraformaldehyde. Samples were embedded in paraffin and ... See full document

... infections in rabbit and cow sen- sory nerve ganglia ...[18,23]. In the present study, the absence of floating neuron-like cells following BoHV-5 infection suggests a low rate of cell ... See full document

... of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and ... See full document

... done in plants that has shown enrichment for methylated cytosines within nucleosomes and display periodicities within the nucleosome core particle (Fig 3A and 3B and S5 Table and S12 ...tie in methylation ... See full document

... mesenchymal stem cells ...use in basic and applied research. They can be obtained, in large quantities, by means of local anesthesia and with the minimum of discomfort; the MSCs have been ... See full document

... DNA from the hESC lines was extracted using DNeasy kit ...starting cells, DNA for Granulosa cells was extracted with the same kit and was subjected to whole genome amplification using REPLI-g mini ... See full document

... role in stem cells ...germ cells (2, 25, 27, ...germ cells and induce expression of molecular markers of spermatogonia (Dazl, Piwil2, Stra8, Rbm, Hsp90a, c-kit), and the and –integrin ... See full document