[PDF] Top 20 Evaluation of the dot enzyme-linked immunosorbent assay in comparison with standard ELISA for the immunodiagnosis of human toxocariasis

... to the procedures described by Boctor et al. (1987) with some ...Strips of NC paper (Sigma), 1 cm wide and 6 cm long were placed on a microtiter 96-well plastic plate and after a slight pressure ... See full document

... being the best sample available for parasite ...as the intermittent pattern of cyst or trophozoite excretion, sensitivities greater than 90% are rarely ...detection of Giardia antigen ... See full document

... - Standard ELISA was performed accord- ing to the procedures described by Roldán et ...(2006) with some ...coated with TES antigens ...well. The microtiter plates were washed ... See full document

... infections of vertebrates with this nematode can result in abdominal ...complications. Human infections are di- agnosed by histological or serological methods because the isolation ... See full document

... fraction of the ...1:100 in PBS-Tween-milk (phosphate buffered solution containing 0.05% of Tween- 20 and 1% skimmed milk) were incubated for 1 h at 37 o ...series of washings, a ... See full document

... (PBS-BSA). The plates were then sequentially incubated with 50 µL biotinylated an- ti-human immunoglobulin G (1:2,000 dilution) (Amer- sham, GE Healthcare Life Sciences, Buckinghamshire, UK) ... See full document

... from the three purification procedures showed identical performance in the standardized ...resulted in a cut-off of 0.433 in the standard plate, considering ... See full document

... zoonosis with a worldwide distribution and is transmitted to animals and humans by infectious eggs from Toxocara canis and Toxocara ...diagnosis. The traditional diagnostic method for this disease relies on ... See full document

... one of the most important endemic mycoses in Latin America; it's usually diagnosed by observation and/or isolation of the etiologic agent, Paracoccidioides brasiliensis, as well as by a ... See full document

... use of immunoblotting in cervico-vaginal mucus samples for the diagnosis of bovine genital campylobacte- riosis in herds with no history of the disease could be a ... See full document

... followed in many investigations of SE outbreaks in poultry relies mainly on culturing the egg contents for SE isolation (yolk, yolk vitelline membrane, ...to the very low frequency ... See full document

... conducted in local laboratories in Ecuador (Calvopiña et al. 2015). The sensitivity of direct microscopic observation is up to ten times lower than the formalin-ether method (Calvo- ... See full document

... following the manufacturer's instructions. The env-nested PCR was performed with the external primers ENV1 (5’-TCT GTG CCA AGT CTC CCA GAT A-3’) and ENV4 (5’-AAC AAC AAC CTC TGG GAA GGG T-3’) ... See full document

... located in the Amazon State and River Iguape (pH ...ted in an Environmental Protection Area in Southern Saint Paul State - ...Brazil. The water samples were collected at sur- face, ... See full document

... procedures with correct virus identification are important for establishing appropriated plant disease control program (JAN et ...2013). The plate trapped viruses together with their specific ... See full document

... role in the diagnosis of intestinal amoebiasis, as well as the history of the ...tests, the subjectivity of the test, the requirement for experi- ... See full document

... aim of this study was to estimate the frequency of human toxocariasis in Cauday district, Cajamarca, Peru, using a dot-ELISA ...total of 256 adult subjects ... See full document

... proportion of asymptomatic dogs, serological meth- ods are essential for CVL ...Although the immu- nofluorescent antibody test (IFAT) is the most widespread diagnostic method, cross-reactivity ... See full document

... and the pH and ionic strength are additional factors that influence on the equilibrium constant ...Therefore, the pH and ionic strength of the working solution were optimized (Table S3 ... See full document

... proportion of asymptomatic dogs, serological meth- ods are essential for CVL ...Although the immu- nofluorescent antibody test (IFAT) is the most widespread diagnostic method, cross-reactivity ... See full document