[PDF] Top 20 Excitability constraints on voltage-gated sodium channels.

... functional constraints bound and shape evolution through an analysis of mammalian voltage-gated sodium ...of sodium channels is to allow the propagation of action ...that ... See full document

... available on the venoms and toxins produced by ...light on their evolutionary relationship with other Tityus toxins that target ion channels considering the primitive status of this subgenus as ... See full document

... all-or-none voltage deflections triggered when the membrane potential of a neuron crosses a ...the voltage at the upstroke of spikes, varies with firing history and input ...of sodium channels ... See full document

... effect on other pharmacological properties of sodium ...based on toxicity assays and binding ex- periments ...toxins on mam- malian VGSCs by studying the modulation of gating prop- erties, not ... See full document

... CNG channels are heteromeric complexes made up of principal and modulatory ...channel on their own, the properties of which can be modified by heteromerization with the A4 subunit or with either of the two ... See full document

... tissue excitability to light was recently ...effect on the electrical properties of cardiomyocyte ...of voltage-gated ion channels, this study examined whether the sensitization effect ... See full document

... stimulation on the axon and the cell ...of voltage-gated calcium channels in response to membrane depolarisation, speed of Fluo-4 response to the increase of calcium concentration, and of ... See full document

... The objective of this study was to investigate PUFAs as reducers of excessive neuronal responses to synchronous input, and thereby as possible anticonvulsive substances. To experimentally test the importance and ... See full document

... through voltage-gated ionic channel. Voltage- gated sodium and potassium channels (Nav and Kv, respectively) are critical to the generation of these action potentials that convey ... See full document

... 7.5. Samples were separated on 10% sodium dodecyl sulfate– polyacrylamide gels by electrophoresis and transferred to nitrocellulose membranes. The primary antibodies used in this study were: ... See full document

... act on the phospholipids and contributes to the destruction of neuronal membranes and brain vascular endothelium, through a mechanism called enzymatic lipid peroxidation (the non enzymatic lipid peroxidation is of ... See full document

... Brain injury due to ischemia is a serious cause of disability and death worldwide. Neuroprotection and brain repair remains a great medical challenge, since pharmacological interventions seem to be ineffective. However, ... See full document

... VI revealed the presence of two major fractions as well as a minor electrophoretic band (results not shown). This fraction was subjected to a reverse phase HPLC. The chromatographic profile of the purification of the ... See full document

... 1. The required data for plate (corrugation pattern, dimensions, area enlargement factor and thermal conductivity), hot and cold fluids (flow rate, inlet temperature, fouling factor and correlations for friction factor, ... See full document

... act on the insect sodium ...action on the insect sodium channel and furthermore, if it could act on mammalian sodium ...tested on the mammalian sodium channel ... See full document

... Gomez et al. (16) showed that PhTx3 decreased KCl-elicited ACh release in MPLM and suggested that PhTx3 acted on calcium channels. Guatimosim and co-work- ers (17) have shown that Tx3-3, one of the isoforms ... See full document

... Flp-In 293 T-REX cell lines expressing DmelOrco and its variants were plated (700,000 cells/well in a 6-well plate, grown for 24 h, and induced with tetracycline (0.1 m g/ml) for 24h and proteins at the cell-surface ... See full document

... actions on heart muscle. Nevertheless, no work on the pharmacological action mechanisms of the AmV in isolated aorta has been ...melittin, on isolated ... See full document

... and lysed in 30 m l 2x Laemmli sample buffer supplemented with 2 mM PMSF. Samples were run on 8 or 10% polyacrylamid gel and blotted onto nitrocellulose membrane. To block non-specific binding sites in Western ... See full document

... capacitors on the AC side of the diode rectifier in the front ...AC voltage, even greater than line ...during voltage sags without any additional energy storage ...output voltage range [3] [4] ... See full document