[PDF] Top 20 Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola

... Kilamba-Kiaxi and Viana since all but 5 patients resided in these munic- ...located in the eastern zone of Kilamba-Kiaxi, near the Viana municipality and primarily serves these two ... See full document

... assignment of clonal isolates by SNVs offered a clue to separate them into local epidemic cases (sub-clonal group II) and nationwide expansion (sub-clonal group I and III) ...subdivision of ... See full document

... 42.7% in 1992 (Silva et al. 1992); another survey determined primary resistance in that state during the nineties to be ...16.5% and secondary resistance between ...47.5% and ... See full document

... association of R-TB and previous treatment was observed. Furthermore, in nine of the hot spot zones studied, there were patients who had lost follow-up or failed treatment, which resulted ... See full document

... used in the molecular epi- demiology of TB, to investigate the population structure of ...M. tuberculosis, focusing on the identification of genotypic lineages and spoligotype ... See full document

... berculosis and mutations related to drug resistance are mainly based on the PCR amplification of target genes or mutations and subsequent analysis of the amplified product by ... See full document

... M. tuberculosis strains are highly clonal. However, SNP analysis of 3R genes seems to be a robust phylogenetic method with very high resolution, even for a generally monomorphic, recent pathogen, such as ... See full document

... association of R-TB and previous treatment was observed. Furthermore, in nine of the hot spot zones studied, there were patients who had lost follow-up or failed treatment, which resulted ... See full document

... the genetic variation among Mycobacterium tuberculosis isolates in the East Azerbaijan Province of Iran and to evaluate the level of and risk factors for recent ... See full document

... M. tuberculosis population can be caused by a re-infection event or by an evolutionary genetic variation of a single infection event (Small et ...the genetic relatedness of the ... See full document

... one of the 9 strains displayed an identical ...level of one locus among the 24 loci analyzed (Table 3) thus, suggesting a possible focal outbreak in Pointe Noire district since 6 strains of ... See full document

... number of compounds have been shown as potential inhibitors of the ...M. tuberculosis efflux systems (Viveiros et ...thioridazine and chlorpromazine and the phenylalkylamine verapamil ... See full document

... sequence-based diversity dataset to further explore the complex interactions between mycobacterial evolution and human population growth and travel ...‘‘Out- of-and-back-to-Africa’’ ... See full document

... to Mycobacterium tuberculosis is a reality worldwide, and its diagnosis continues to be difficult and time ...use of rapid molecular tests. We evaluated the routine use (once a week) ... See full document

... strains of Mycobacterium tuberculosis have attracted worldwide attention because of their wide geographical distribution and global ...strains in South America. We aimed to ... See full document

... Tris-base and 1 mM EDTA, pH 8.0). The suspension was mixed with 50 μL of lysozyme (10 mg/mL) and 10 μL of RNAse A and incubated at 37°C for 2 ...μL of proteinase K (10 mg/mL) at ... See full document

... component of respiratory chain of Mtb—thus raising the hypothesis that this is the main molecular target of TZ and making it also effective against latent TB ...reaction of the electron ... See full document

... Background: Tuberculosis (TB) causes about 3 million of death per ...mechanism of defense against Mycobacterium tuberculosis. The role of gamma-delta (/9) T lymphocytes in ... See full document

... assay of Inter- delta genomic sequences. Experimental parameters of PCR to identify were optimized and optimal simplified method of DNA extraction from dried preparations of yeast ... See full document

... poverty, drug addiction and a lack of access to health programs (Watterson et ...14% of new TB cases world- wide are MDR, this number can reach up to 50% among previously treated patients ... See full document