[PDF] Top 20 Human stem cell-derived neurons: a system to study human tau function and dysfunction.
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Human stem cell-derived neurons: a system to study human tau function and dysfunction.
... stabilization and axonal transport [1,2]. The human tau gene is located in chromosome 17, it consists of 16 exons that are alternatively spliced to generate six different tau ...region ... See full document
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Efflux protein expression in human stem cell-derived retinal pigment epithelial cells.
... mRNA and protein ...cells, and declined thereafter in fully mature cells with a cobblestone ...first study to confirm that MRP1 expression fluctuates depending on the maturation ...cells, ... See full document
10
Stimulation of GABA-induced Ca2+ influx enhances maturation of human induced pluripotent stem cell-derived neurons.
... fluorimeter system (Cairn Research, Faversham, Kent, ...U.K.) and continuously perfused using an RSC, rapid perfusion system (RSC160, Intracel RSC160, Intracel, Royston, ...γ40 and γ80 nm ... See full document
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Estrogen receptor beta-selective agonists stimulate calcium oscillations in human and mouse embryonic stem cell-derived neurons.
... emissions and comparison with Fura-2 standards ...[46,47] to detect peaks in [Ca2+]i ...0.53 and 0.39. A smoothing window of 25 sec was used to determine segmented baseline ...of ... See full document
17
Primary cilia: the chemical antenna regulating human adipose-derived stem cell osteogenesis.
... Adipose-derived stem cells (ASC) are multipotent stem cells that show great potential as a cell source for osteogenic tissue replacements and it is critical to understand the ... See full document
11
In Vitro Reconstruction of Neuronal Networks Derived from Human iPS Cells Using Microfabricated Devices.
... way to overcome these problems in neuronal ...chamber to separate neurites from cell bodies ...due to its size, the Campenot chamber is not suitable for culturing any neurons, ... See full document
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Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells.
... commitment to functional CD34+ progenitor cells from human iPSC is crucial for the clinical application of ...stromal cell co-culture or EB–based systems [9,23–25,29], the induction efficacy ... See full document
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Use of Ferritin Expression, Regulated by Neural Cell-Specific Promoters in Human Adipose Tissue-Derived Mesenchymal Stem Cells, to Monitor Differentiation with Magnetic Resonance Imaging In Vitro.
... this study was to establish a method for monitoring the neural differentiation of stem cells using ferritin transgene expression, under the control of a neural-differentia- tion-inducible promoter, ... See full document
14
Male germ-like cell differentiation potential of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells in co-culture with human placenta cells in presence of BMP4 and retinoic acid
... cells and germ cells, which can be stimulated by ...appear to be necessary for spermatogenesis and the development of spermatocytes through early stages of ...ESCs and bone marrow cells into ... See full document
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Variations of X chromosome inactivation occur in early passages of female human embryonic stem cells.
... development and cell physiology. Human embryonic stem cells (hESCs) derived from inner cell mass (ICM) of blastocyst stage embryos have been used as a model system ... See full document
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Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Transplants Improve Recovery after Cervical Spinal Cord Injury
... Morphometry and cell quantification analyses revealed a number of significantly improved histological outcomes in the transplants compared to ...forelimb function in both transplants and ... See full document
12
Human embryonic mesenchymal stem cell-derived conditioned medium rescues kidney function in rats with established chronic kidney disease.
... ) and gene expression profile, were generated by trypsinization and propagation of hESCs from either HuES9 hESC line or H1 hESC line in feeder- and serum-free selection media ...doublings. To ... See full document
12
A novel zebrafish xenotransplantation model for study of glioma stem cell invasion.
... Invasion and metastasis of solid tumors are the major causes of death in cancer ...Cancer stem cells (CSCs) constitute a small fraction of tumor cell population, but play a critical role in tumor ... See full document
9
Characterization of human adipose-derived stem cells
... aspirated and the lask was washed using a PBS solution supplemented with ethylenediamine-tetra acetic acid (EDTA) ...discarded and 3 ml of a solution composed by tripsin ...lask and left for two ... See full document
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Screening for osteogenic activity in extracts from Irish marine organisms: the potential of Ceramium pallidum
... DMSO and ethanol, which may have been due in part to the use of a non-toxic solvent and the ability this afforded to use higher treatment ...compounds and therefore it is also not ... See full document
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Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.
... Immunocytochemistry and staining procedures were as de- scribed previously ...single and double ...reactivity and non-specific ...1:50, and as secondary antibodies: Alexa Fluor 488 Goat ... See full document
11
Genetic background drives transcriptional variation in human induced pluripotent stem cells.
... these cell lines are heterogeneous, which presents a limitation for their use in disease modeling and personalized ...medicine. To explore the basis of this heterogeneity we generated 25 iPS ... See full document
11
Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.
... were derived by transducing skin cells with a modified version of our ‘‘Hit and Run’’ lentiviral vector [19] carrying Oct4, Sox2, Klf4 and c-Myc cDNAs driven by the EF-1a ...cells to produce ... See full document
10
Protective effects of mesenchymal stem cells with CXCR4 up-regulation in a rat renal transplantation model.
... applications, and thus, the localization of MSCs is crucial for successful ...homing and engraftment of MSCs to target ...humans and rats [45,46]. CXCR4 and SDF-1 levels are both ... See full document
13
Generating a non-integrating human induced pluripotent stem cell bank from urine-derived cells.
... pluripotent stem cells that can undergo unlimited self-renewal while maintaining the potential to differentiate into all three germ ...layers. Human iPS cells hold great potential to be used ... See full document
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