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[PDF] Top 20 Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean

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Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean

Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean

... the analysis of gene-expression stability and rank; the best reference gene for soybean was selected by the GeNorm application (Vandesompele et ...mean of the ... See full document

8

Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.

Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.

... candidate reference genes, such as actin, a-tubulin, translation elongation factor, and ADP-ribosylation factor, belong to different ...families. Genes in the same family have conserved ... See full document

11

Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.

Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.

... lot of attention as a forage and bioenergy crop during the past few ...Gene expression studies are in progress to improve new traits and develop new ...cultivars. Quantitative real ... See full document

12

Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.

Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.

... stresses, soybean plants activate numerous signaling pathways to respond to the adverse environmental stimuli ...the expression of various ...responsive genes, stably expressed ... See full document

10

Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.

Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.

... potential reference genes were chosen for being frequently used as endogenous controls in expression studies by different authors [6,9,18]: beta-actin (ACTB), beta-2 microglobulin ... See full document

9

Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).

Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).

... Gene expression analysis plays a major role in understanding of the complex regulatory net- works and identification of genes relevant to new biological processes ... See full document

16

Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).

Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).

... P-glycoprotein expression levels was higher in resistant than in susceptible strain had been previously found by Lou et al [21] using Western blotting and Vanadate-sensitive ATPase ...assessed ... See full document

9

Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.

Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.

... used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as ... See full document

10

Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.

Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.

... identified by microarray analyses in Arabidopsis thaliana and soybean that show highly stable expression levels ...These reference genes include SAND family protein (SAND), ... See full document

10

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR

... quantification of accurate gene expres- sion have an increasingly important role in studies aiming for the reliable examination of expression profiles gener- ated by high-throughput ... See full document

11

Identification and expression analysis of cytokinin metabolic genes in soybean under normal and drought conditions in relation to cytokinin levels.

Identification and expression analysis of cytokinin metabolic genes in soybean under normal and drought conditions in relation to cytokinin levels.

... number of exons (10, 9 and 11, respectively), while five GmIPT members lack ...GmIPT genes possess two or three exons (Figure 1A, Table ...observed in genes encoding IPTs from Arabidopsis ... See full document

15

Morphophysiological and molecular responses of sugarcane genotypes to water stress

Morphophysiological and molecular responses of sugarcane genotypes to water stress

... higher in that genotype than in the susceptible ...reduction in stalk length in this genotype, while no differences were observed for the tolerant ...total of 15 ... See full document

67

Genomic signatures predict poor outcome in undifferentiated pleomorphic sarcomas and leiomyosarcomas.

Genomic signatures predict poor outcome in undifferentiated pleomorphic sarcomas and leiomyosarcomas.

... candidate genes from this region had been studied in the context of ...expressed in all normal human tissues with increased expression in the ovary, lung, spleen, testis, and ... See full document

11

Estudo de função de HIPK2 durante o desenvolvimento embrionário

Estudo de função de HIPK2 durante o desenvolvimento embrionário

... Quatro regiões do transcrito de Hipk2 foram selecionadas como potenciais locais de ribossondas de hibridações in situ. A figura 4 mostra um esquema da seqüência de Hipk2 murina e os locais escolhidos para desenho ... See full document

92

Melissa officinalis extract inhibits laser-induced choroidal neovascularization in a rat model.

Melissa officinalis extract inhibits laser-induced choroidal neovascularization in a rat model.

... AMD in humans. It is a model of acute injury and inflammation, rather than one of long standing senile degeneration and chronic inflammation ...use in traditional herbal ...injections ... See full document

11

Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.

Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.

... case of endothelial cells of the blood-brain barrier (BBB) of patients afflicted with neurodegenerative disorders such as Alzheimer's, Parkinson's, or multiple sclerosis, as well as in cases ... See full document

22

Inhibitory effect of tanshinone IIA on rat hepatic stellate cells.

Inhibitory effect of tanshinone IIA on rat hepatic stellate cells.

... involved in inflamma- tory ...downregulated by Tan ...suppressed by both Tan IIA (10 m M) and Sal B (200 m M, Figure ...comparison in the Luciferase ...expressions of IL-1b, TNF-a, ... See full document

10

Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR

Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR

... set of four primers (B3, F3, BIP and FIP), recognizing six distinct sequences within a target DNA in total (Fig ...groups of primers were designed and filtered to select the best group depending on ... See full document

9

Efeitos das dietas em Ômega-3 OU Ômega-6 na expressão gênica de neoplasias mamárias de ratas Sprague-Dawley sob o tratamento com tomoxifeno

Efeitos das dietas em Ômega-3 OU Ômega-6 na expressão gênica de neoplasias mamárias de ratas Sprague-Dawley sob o tratamento com tomoxifeno

... efficacy of tamoxifen (Tam) against N-methyl-N-nitrosourea (MNU)-induced rat mammary ...gene expression of mammary tumors depending upon the type of dietary fat fed to the ...rich in ... See full document

87

The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos)

The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos)

... inhibitor in the egg white. In the present study, real-time quantitative PCR and Western-Blot were used to analyze different expression pattern of OIH in ... See full document

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