[PDF] Top 20 Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
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Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... levels and obtain more accurate quantitative real-time RT-PCR (qRT-PCR) data, normalization relative to reliable reference gene(s) is ...required. Drosophila ... See full document
9
Gene expression profile and toxic effects in human bronchial epithelial cells exposed to zearalenone.
... Validation of differentially expressed genes by qPCR Disturbingly, many of the pro-inflammatory responsive genes were down-regulated while those anti-inflammatory genes were ... See full document
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Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.
... levels of DREB2 peaked at 3–6 h and then began to decline at 12 h in roots under salt stress conditions when UNK2 and SAND were used for normalization (Figure ...conditions in Caragana ... See full document
10
Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient
... first time the use of qPCR to validate sexing bovine sperm submitted to density gradient centrifugation was ...proportion of X and Y sperm in semen samples before centrifugation without ... See full document
8
Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).
... role in understanding of the complex regulatory net- works and identification of genes relevant to new biological processes ...measurement, real time ... See full document
16
Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
... cause of tuberculosis in ...occurrence of HIV, presenting itself as an important opportunistic infection as- sociated with acquired immunodeficiency syndrome ...a real-time PCR ... See full document
7
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... suitable reference gene for expression normalization in ...NormFinder and deltaCt across our four experimental ...involved in the process of protein translation, eIF-4a had the same ... See full document
10
Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.
... potential reference genes were chosen for being frequently used as endogenous controls in expression studies by different authors [6,9,18]: beta-actin (ACTB), beta-2 microglobulin (B2M), guanine ... See full document
9
Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR
... organisms in environmental samples directly from environmental DNA ...accurate and culture- ...regions in the genome [13,14] ...located in rrs genes, and finer taxonomic ... See full document
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Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
... used in the qRT-PCR analyses are presented in Table ...pairs and a single amplifica- tion product of the expected size for each gene was obtained by electrophoresis on a 2% agarose ... See full document
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Validation of reference genes for quantitative RT-PCR normalization in Suaeda aralocaspica, an annual halophyte with heteromorphism and C4 pathway without Kranz anatomy
... ment of salinity for optimal growth, ...function of stress tolerance-related genes, and which can highly improve the quantification of gene expression profiles (Fan et ...internal ... See full document
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Selection and assessment of reference genes for quantitative PCR normalization in migratory locust Locusta migratoria (Orthoptera: Acrididae).
... insect and has caused widespread economic damage to crops as a migratory ...pattern of functional genes in L. migratoria have drawn focus in recent years, especially with the release ... See full document
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Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
... the genes tested were designed using Oligo Primer Analysis software version ...analysis, and verified for the absence of non-specific ...provided in results. RTqPCR runs were performed ... See full document
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Validation of reference genes for real-time PCR of reproductive system in the black tiger shrimp.
... variation of expression levels between different individuals during pregnancy [32], with developmental stages [33,34] and during the cell cycle of human cells [35], which agrees with our result when ... See full document
10
Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... analysis of gene-expression stability and rank; the best reference gene for soybean was selected by the GeNorm application (Vandesompele et ...(M) and a normalization factor (NF) based on the ... See full document
8
Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
... comparison of two different computer programs, geNorm [22] and NormFinder [23], was used to select the best internal controls for normalization of gene expression studies in ...comparison ... See full document
12
Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes
... prior validation, the three reference genes used in sheep from temperate regions cannot be employed in studies involving tropical ...that in Brazilian Somali sheep infected with ... See full document
5
Selection of suitable housekeeping genes for real-time quantitative PCR in CD4(+) lymphocytes from asthmatics with or without depression.
... with and without depressive disorders can lead to the identification of genes implicated in such diseases and provide added insight into the underlying pathophysiological ... See full document
9
Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... total of 14 genes that have been used in the studies of cucurbit and other crops were selected for use as candidate reference genes for ...genome and unigene ... See full document
11
Identification of Suitable Reference Genes for Investigating Gene Expression in Anterior Cruciate Ligament Injury by Using Reverse Transcription-Quantitative PCR.
... number of candi- date reference genes; it is likely that some other genes may also be used as internal references for gene expression studies in ACL samples from patients with or ... See full document
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