[PDF] Top 20 The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers.
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The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers.
... and the absorbance of each well was read at 450 nm with an UVmax ...For the direct binding assay, wells of ELISA plates were coated with 5 m g/ml native double stranded calf thymus (CT) ... See full document
7
Viral reverse transcriptases show selective high affinity binding to DNA-DNA primer-templates that resemble the polypurine tract.
... loss of binding and washed 26 with 25 mM Tris-HCl (pH ...extracted by resuspending the nitrocellulose filter in 500 m ls of 10 mM Tris- HCl (pH ...14 of MuLV SELEX were performed ... See full document
10
Adiabatic Differential Scanning Calorimetric Study of Divalent Cation Induced DNA - DPPC Liposome Formulation Compacted for Gene Delivery
... delivers DNA into cultured cells by electrostatic mechanisms of binding to their negatively charged ...cells by various routes, such as through endocytic pathway and direct ... See full document
5
The Trypanosoma cruzi nucleic acid binding protein Tc38 presents changes in the intramitochondrial distribution during the cell cycle
... with the propensity to melt in order to facilitate the landing of the replication machinery while single stranded DNA binding proteins assist in the main- ... See full document
11
SNBRFinder: A Sequence-Based Hybrid Algorithm for Enhanced Prediction of Nucleic Acid-Binding Residues.
... As the number of experimentally solved protein-nucleic acid complexes increases steadily, a variety of structure-based algorithms have been proposed to predict DNA- or ... See full document
23
Inhibition of TDP-43 aggregation by nucleic acid binding.
... Aggregation of the TDP-43 Protein in Cell-free Systems The eukaryotic cell-free system derived from the rabbit reticulocyte lysate (TNT T7 Quick-Coupled Transcription/ Translation System, ... See full document
11
Functional importance of the DNA binding activity of Candida albicans Czf1p.
... with the model that Czf1p exerts its positive influence on filamentation and relief of inhibition by Efg1p, by acting at promoters via its DNA-binding ...at the ... See full document
10
MicroRNA regulation by the DNA and RNA binding proteins EWS and FUS
... compared to the elongated shape of KOFUS1 and KOControl treatments (Supplementary Figure ...any of the previous literature, this issue was investigated ...further. The siRNAs ... See full document
54
Affinity chromatography in plasmid DNA purification for therapeutic applications
... see the retention pattern chromatograms achieved at 5ºC for the different oligonucleotides ...represents the elution profiles performed with a particular oligonucleotide, testing different salt ... See full document
182
A Method To Find The Area Of Sector Without The Usage Of Angle Made By The Chord
... then the theorem is ...Thus the equation of circle is x²+y²=r².The equation of the chord at ‘a’ distance from center is ax-ry- ar=0 or Y= ... See full document
2
From the field to the lab: best practices for field preservation of bat specimens for molecular analyses.
... sampling of bats usually involves obtaining patagial biopsy samples [20–22] or feces ...easy to transport, and have been the preferred method of nonlethal bat tissue preservation in the ... See full document
12
Cooperative DNA Recognition Modulated by an Interplay between Protein-Protein Interactions and DNA-Mediated Allostery.
... models of the OCT4-SOX2-UTF1 ternary complex with the human UTF1 sequence (5'-CAGGCATTGTTATGCTAGCGGAACTCC-3') using our previous models of OCT4- SOX2 complexes bound to a consensus ... See full document
23
Detection of cell carcinogenic transformation by a quadruplex DNA binding fluorescent probe.
... For the scratch wound assay, cells were cultured as confluent monolayers and were wounded by removing a 1 cm strip of cells across the well ...used to record the cell migration ... See full document
10
Expression, purification and DNA-binding activities of two putative ModE proteins of Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
... from the modA1 ...gion of the genome, probably forming a single operon with another modE-like gene, modE2, immediately upstream and transcribed divergently from ...modA2. The organization ... See full document
8
ATP-independent cooperative binding of yeast Isw1a to bare and nucleosomal DNA.
... order to directly investigate the action of an ISWI family member on DNA, we have studied the behaviour of the yeast complex Isw1a on single DNA molecules using an ... See full document
13
Characterization of a novel ArsR-like regulator encoded by Rv2034 in Mycobacterium tuberculosis.
... Self-regulation of Rv2034. (A) The putative promoter region of Rv2034 was cloned upstream of the HIS3-aadA reporter genes in the bacterial one-hybrid reporter vector pBXcmT, as ... See full document
10
Functional diversification of paralogous transcription factors via divergence in DNA binding site motif and in expression.
... followed by functional diversification of the duplicated genes is a major driver of ...in the case of immunity related ...changing the activity of the gene ... See full document
12
Mutation of androgen receptor N-terminal phosphorylation site Tyr-267 leads to inhibition of nuclear translocation and DNA binding.
... recruitment to the chromatin of truncated AR protein and transcription of AR responsive genes are impaired by the Y267F ...for binding of FLAG-tagged truncated AR ... See full document
19
Low Resolution Structures of the Retinoid X Receptor DNA-binding and Ligand-binding Domains Revealed by Synchrotron X-ray Solution Scattering
... with the two DBDs occupying the tips of both arms of the ...U. The DBDs are closely positioned in such a way that they could contact DNA without major conformational ... See full document
9
Thermodynamic state ensemble models of cis-regulation.
... is to develop models that accurately predict a gene’s expres- sion from its surrounding regulatory ...class of such models, thermodynamic state ensemble models. We describe the biochemical derivation ... See full document
10
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