[PDF] Top 20 MATERIALS AND METHODS Bacterial strain isolation and cultivation
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MATERIALS AND METHODS Bacterial strain isolation and cultivation
... 4ºC, and centrifuged at 29,000 x g for 1h at ...NaCl and clarified at 28,000 x g for 20 min at ...buffer and eluted with a NaCl linear gradient (100 - 800 ...NaCl, and clarified by ... See full document
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MATERIALS AND METHODS Cultivation of the microorganism
... The results obtained in the group of experiments 7 to 9, where the Catlin 6 medium was used (an average of 0.095 g/L and 0.067 respectively) show that this medium can be considered an interesting possibility for ... See full document
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MATERIALS AND METHODS Cultivation of the microorganism
... organic and inorganic nitrogen in the medium were measured during ...the cultivation time, whereas the organic nitrogen consumption was linearly related to cell growth, with constant yield factors (average ... See full document
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MATERIALS AND METHODS Strain Cultivation
... The hydrolysis of lignocellulosic biomass to fermenting sugars, which can be used for ethanol biosynthesis, is one of the possibilities for production of alternative energy sources. Annually about 7 million tons of ... See full document
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MATERIALS AND METHODS Strain and maintenance
... flask cultivation of Aurantiochytrium ...C/N-ratio and absolute docosahexaenoic (DHA)-levels fell in narrow range (5-6 ...rate and/or incomplete glucose utilization occurred. Glucose and FA- ... See full document
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MATERIALS AND METHODS Bacterial isolates
... Sung and Collins (41) observed that a higher proportion of low passage MAP clinical isolates were sensitive to heat when compared to high passage ...8672 and 8819 mostly responded to temperature stress, ... See full document
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MATERIALS AND METHODS Bacterial strains
... 8ºC and 15ºC, and used for critical dilution ...those bacterial cells were not only survivors but resistant to ...AcH and nisin A) and verified similar antimicrobial patterns ranging ... See full document
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MATERIALS AND METHODS Bacterial cultures
... of bacterial isolates was extracted using the Puregene DNA Isolation Kit (Gentra Systems, QIAGEN ...pA and pH as previous described ...System and then directly sequenced in both directions by ... See full document
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MATERIALS AND METHODS Screening and isolation conditions
... positive and hydrolysis of gelatin that are typical ones for this genus (6, ...18). Strain KNOUC808 showed positive in hydrolysis of PNPG and ONPG, and utilization of lactose, and ... See full document
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MATERIALS AND METHODS Bacterial strains
... SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ...ipaA and ipaC in these ...M90T and FBC124- 13 were compared, ten amino acids changes could ... See full document
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MATERIALS AND METHODS Bacterial strains
... Brazil and was kindly donated by Prof. Marcelo Brocchi. The reference strain was obtained directly from American Type Culture Collection (ATCC), ...The bacterial strains were kept at - 70ºC in tubes ... See full document
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MATERIALS AND METHODS Bacterial strains and media
... pepF::pNPTS138 strain (SP2008) showed no growth defects, with a doubling time of 3 h, equivalent to the wild type strain NA1000, and normal swimming behaviour was observed by light ...phase, ... See full document
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MATERIALS AND METHODS Isolation and characterization of Gram-positive and Gram- negative bacterial strains
... studied and comprehensively reviewed (28, 62, 69, 74, ...swelling, and reduces the normal functioning of membrane- associated proteins (52, 74, 84, ...stability and membrane structure, causing a loss ... See full document
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MATERIALS AND METHODS Bacterial strains and media
... each strain, the Tn5 insertion site was determined by DNA ...phenylalanine and tyrosine ...(16-3A) and the other is separated by 180 bp (25-3C) ... See full document
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MATERIALS AND METHODS Bacterial strains and media
... Growth and fermentation of sugarcane juice to ethanol by KO11 and P2 required addition of ...micronutrients and thiamine promoted better ethanol ...recombinant strain that gave rise to KO11, ... See full document
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MATERIALS AND METHODS Bacterial Strains
... coco-bacillus and an intracellular opportunistic pathogen which causes pneumonia in ...environment and has been isolated from several sources, as soil, feces and gut from health and sick ... See full document
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MATERIALS AND METHODS Isolation and maintaining of cultures
... cutinase and lipase belong to the esterase group of enzymes (EC ...food and cosmetic ...plants and perform a pre-selection of molds showing esterase producing ...lipolytic and cutinolytic ... See full document
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MATERIALS AND METHODS Bacterial strains
... plants) and their transformed strains were used to study their influence on Triticum aestivum ...(46%) and fresh weight (39%) in non-inoculated ...wild and transformed strains stimulated germination, ... See full document
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MATERIALS AND METHODS Isolation and identification of bacterial strains
... cysts and nauplii were obtained via decapsulation as described by Sorgeloos et ...filtered and autoclaved sea water (FASW) over a 50 m sterile filter ...filtered and autoclaved sea water ...capped ... See full document
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MATERIALS AND METHODS Bacterial strain
... Bacterial strain MP6 was isolated from root nodule of Mimosa pudica was characterized morphologically, biochemically and physiologically according to Holt et ...(20) and identified as ... See full document
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