[PDF] Top 20 MATERIALS AND METHODS Strain and maintenance
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MATERIALS AND METHODS Strain and maintenance
... C/N-ratio and absolute docosahexaenoic (DHA)-levels fell in narrow range (5-6 ...rate and/or incomplete glucose utilization occurred. Glucose and FA- fluxes based on fat-free biomass peaked at a C/N ... See full document
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MATERIALS AND METHODS Strain and growth conditions
... inoculated and spread on sterile strips of dialysis membranes (1 cm x 2 cm) overlying SMM supplemented or not, with a benzene compound and incubated at 30ºC for different periods of ...solution, and ... See full document
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MATERIALS AND METHODS Organism, maintenance and culture conditions
... extracellular and intracellular fructosyltransferase and hydrolytic enzymes ...extracellular and intracellular fructosyltransferase production were recorded after day 4 and day 3 of ... See full document
6
MATERIALS AND METHODS Microorganism maintenance and culture conditions
... biotechnological and environmental processes has had increasing interest due to its efficiency, selectivity and mainly for being environmentally healthful, but these applications require a great volume of ... See full document
5
MATERIALS AND METHODS Organism, Growth and Maintenance
... fungi and bacteria are employed for the production of proteases and alpha amylases but the choice of organism mainly depends upon the application of the enzyme ...thermostability and heat sensitivity ... See full document
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MATERIALS AND METHODS Microorganism and culture maintenance
... Isomaltulose [O-α-D-glucopiranosyl-(1→6)-β-D-frutofuranose] is a reducing disaccharide commercially obtained by the enzymatic conversion from sucrose catalyzed by a microbial glucosyltransferase as shown in Fig. 1. ... See full document
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MATERIALS AND METHODS Isolation and maintenance of microbes
... 5.2) and 100µl of culture supernatants (6) were incubated for 1 h at 50 0 ...stock and prior to use, it was diluted with nine volume of glacial acetic acid) was added in each tubes and incubated at ... See full document
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MATERIALS AND METHODS Yeast culture and its maintenance
... Statistical optimization of a process or formulation of a fermentation medium can overcome the limitations of classical empirical methods. A successful application of RSM to enhance exoinulinase production from K. ... See full document
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MATERIALS AND METHODS Organism, maintenance and culture condition
... histidine and cysteine induced ß-glucosidase synthesis to certain ...temperature and pH for ß-glucosidase activity was 50°C and ...juice and wine ... See full document
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MATERIALS AND METHODS Microorganisms and maintenance
... extract and peptone were the best nitrogen sources for highest production of inulinases I & II at five and seven days of incubation ...gel-filtration and ion-exchange chromatography with ...75 ... See full document
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MATERIALS AND METHODS Microorganism and maintenance
... 40 and 50 ºC for mushrooms fresh, dehydrated mushrooms and powdered dehydrated mushrooms, using the saline solutions static method (Rizvi, ...desiccator and periodically weighed until constant ... See full document
8
MATERIALS AND METHODS Microorganisms and maintenance
... acid and hydrogen peroxide in the presence of oxygen and ...marker and viral antigens ...enzymes and eventual secondary metabolites with potential anti-microbial and anti-parasitic ... See full document
7
MATERIALS AND METHODS Strain Cultivation
... The substrate specificity of endoglucanase was tested with 10 mg/mL carboxymethyl cellulose (CMC), cellobiose, and methyl glycoside (Sigma) by determination of the reducing sugars released. When 1.0 mM ... See full document
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MATERIALS AND METHODS Virus strain and aphid colony
... lamina and were transmitted by the ...plants, and efficient transmission was observed when these particles were microinjected in the hemocoel, indicating that these mutations in the RTD interfere with the ... See full document
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MATERIALS AND METHODS Materials
... The maintenance of the characteristics of the biomaterial is important for a good heart valve ...drying and secondary ...size and, consequently, the primary and secondary drying ...temperature ... See full document
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MATERIALS AND METHODS Raw materials and evaluated treatments
... type and additive ...micelles and the wood retraction (MELO et ...shape and sizes of wood particles bonded with a synthetic adhesive and consolidated under pressure and heat (FREIRE et ... See full document
8
MATERIALS AND METHODS Yeast strain and culture condition
... Gdh activity was observed for cells growing on glutamate, followed by 2-fold decrease in leucine and 1.8-fold decrease in glutamine, when compared to ammonia-growing cells (Table 2). However, glutamine seems to ... See full document
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MATERIALS AND METHODS Bacterial strain isolation and cultivation
... R and F are the amplification products using, respectively, the Reverse and Forward ...U and D are the same amplification products Undigested and Digested, ... See full document
5
MATERIALS AND METHODS Fungal strain, media and growth conditions
... flasks and aerated fermenter cultures have been ...mutan and 0.05% peptone and inoculated with 20% of the 72-h mycelium as ...synthesis and could be utilized as an inexpensive and ... See full document
10
MATERIALS AND METHODS Raw Materials and tannin estimation
... fungal strain identified as Penicillium atramentosum KM was used for tannase production under solid-state fermentation (SSF) using different agro residues like amla (Phyllanthus emblica), ber (Zyzyphus ... See full document
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