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[PDF] Top 20 MATERIALS AND METHODS Bacterial strains, growth conditions and DNA extraction

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MATERIALS AND METHODS Bacterial strains, growth conditions and DNA extraction

MATERIALS AND METHODS Bacterial strains, growth conditions and DNA extraction

... penem and carbapenem -lactam antibiotics in ...threonine; and U, ...superfamily and utilize the free energy of ATP hydrolysis to expel drugs from the cell (39, ...penem and carbapenem ... See full document

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MATERIALS AND METHODS Bacterial strains and culture media

MATERIALS AND METHODS Bacterial strains and culture media

... The filter sterilized, crude extract of the BLS from C. piscicola L 103 had a bacteriostatic effect against L. monocytogenes, when inoculated on salmon pieces, as no significant growth (p>0.05) of the pathogen ... See full document

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MATERIALS AND METHODS Fungal isolates and growth conditions

MATERIALS AND METHODS Fungal isolates and growth conditions

... 27. Sambrook, J.; Fritsch, E.F.; Maniatis, T. Molecular cloning: A Laboratory Manual. New York, Cold Spring Harbor Laboratory, 1989. 28. Sanger, F.; Nicklen, S.; Coulson, A.R. DNA sequencing with chain- ... See full document

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MATERIALS AND METHODS Fungal isolates and growth conditions

MATERIALS AND METHODS Fungal isolates and growth conditions

... laboratory methods “amplification of genomic DNA using random primers, enzyme activity staining, and colony size determination” have been used to discriminate between highly virulent (HV) and ... See full document

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MATERIALS AND METHODS Bacterial strains

MATERIALS AND METHODS Bacterial strains

... while growth occurred at higher incubation temperatures from 20 to 37 ...APC growth curve showed cells entering the stationary growth phase from week 2 onwards until the end of the experiment, a ... See full document

8

MATERIALS AND METHODS Bacterial strains and antimicrobial screening

MATERIALS AND METHODS Bacterial strains and antimicrobial screening

... proline and trehalose on the two strains has shown only at inhibitory ...medium and in ISP5 medium. GB and proline are two major organic osmolytes that accumulate in a variety of plant species ... See full document

11

MATERIALS AND METHODS Organism and growth conditions

MATERIALS AND METHODS Organism and growth conditions

... samples and identified as Pseudomonas and Arthrobacter (Strains 3B, 4B, 7B, 21B, 18E and 21D), were tested for the yield of polysaccharides produced during growth in two culture media: ... See full document

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MATERIALS AND METHODS Bacterial strains

MATERIALS AND METHODS Bacterial strains

... inoculum and antibiotics were dispensed into each well of the microtitre plate ...visible growth on agar plates and incubating for 18–24 ...control strains were included in duplicate in each ... See full document

8

MATERIALS AND METHODS Bacterial strains

MATERIALS AND METHODS Bacterial strains

... sterilized and used to test the activity of bacteriocin against Listeria monocytogenes 1/2a, at 8ºC and ...1 and L. sakei ATCC 15521 and the listerial populations were determined after 0, 5 ... See full document

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MATERIALS AND METHODS Bacterial strains

MATERIALS AND METHODS Bacterial strains

... plant growth promotion are present on chromosome instead of ...bacterium and can be further used for plant-microbe ...in growth stimulation are present on plasmids or on ...isolated and ... See full document

10

MATERIALS AND METHODS Microorganism and growth conditions

MATERIALS AND METHODS Microorganism and growth conditions

... the bacterial genus Rhodococcus are glycolipids with tensoactive properties ...The bacterial cell wall of Rhodococcus has a highly organized and complex cell envelope, formed by a 30 to 54 carbon ... See full document

9

MATERIALS AND METHODS Bacterial strains and media

MATERIALS AND METHODS Bacterial strains and media

... localization and virulence. We have cloned and characterized the gene of the metalloendopeptidase PepF from the aquatic bacterium Caulobacter ...any growth defect, but the mutant strain possesses ... See full document

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MATERIALS AND METHODS Isolation and identification of bacterial strains

MATERIALS AND METHODS Isolation and identification of bacterial strains

... cysts and nauplii were obtained via decapsulation as described by Sorgeloos et ...filtered and autoclaved sea water (FASW) over a 50 m sterile filter ...filtered and autoclaved sea water ...capped ... See full document

9

MATERIALS AND METHODS Bacterial strains, bacteriophage, media and culture conditions

MATERIALS AND METHODS Bacterial strains, bacteriophage, media and culture conditions

... Bacteria and phage preparations: Two different populations of ...10 and 10 2 cfu/mL, were ...Brazil), and 10 µl of the sample was spread onto BHI-YE agar ...h and the colonies were counted on ... See full document

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MATERIALS AND METHODS Bacterial growth and degradation media

MATERIALS AND METHODS Bacterial growth and degradation media

... Various methods for the determination of salicylic acid have been ...These methods vary from colorimetry, fluorimetry and chromatography to ion selective electrodes and voltametric ...assay ... See full document

6

MATERIALS AND METHODS Fungal and bacterial strains

MATERIALS AND METHODS Fungal and bacterial strains

... Overexpression and purification of MBP fusion proteins Selected clones of Vlchit1 were grown at 37ºC in ...the growth medium to repress the maltose genes on the chromosome of the ...0.4 and 0.6. Both ... See full document

7

MATERIALS AND METHODS Bacterial strains and media

MATERIALS AND METHODS Bacterial strains and media

... OD and ethanol determination throughout the 96h incubation ...11750g and supernatants were stored at -20ºC for ethanol ...pH and amount of 2M KOH used were recorded several times during the ... See full document

10

MATERIALS AND METHODS Bacterial strains and media

MATERIALS AND METHODS Bacterial strains and media

... pH and UV light at longer time points, suggesting that despite the fact that the nadB gene is not essential for Caulobacter, its loss causes it to be more sensitive to some ... See full document

6

MATERIALS AND METHODS Bacterial cultures and growth media

MATERIALS AND METHODS Bacterial cultures and growth media

... peroxide and bacteriocin or bacteriocin like compounds ...the growth of diverse Gram positive and Gram negative bacterial strains ...born and spoilage pathogen especially ... See full document

5

MATERIALS AND METHODS Bacterial strains

MATERIALS AND METHODS Bacterial strains

... invading and persisting in the blood, differences in the degree of bacterial susceptibility to serum determine whether a microorganism can infect and persist in a given organism ...faecalis ... See full document

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