[PDF] Top 20 Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense.
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Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense.
... Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique that rapidly amplifies target DNA under isothermal ...gene of Trypanosoma brucei ... See full document
8
Loop-Mediated Isothermal Amplification for Laboratory Confirmation of Buruli Ulcer Disease-Towards a Point-of-Care Test.
... features of LAMP technology are attributable to the Bacillus stearothermophilus- derived Bst polymerase, which is characterized by strand-displacement activity (without 5’-3’ exo- nuclease activity), enzyme ... See full document
14
Rapid and sensitive detection of Plesiomonas shigelloides by loop-mediated isothermal amplification of the hugA gene.
... positive amplification of ...the detection limit of LAMP in human stool, serial 10-fold dilutions of a mid-log-phase culture of ...plating method, resulting in spiking ... See full document
6
Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR
... and rapid examination system for the detection of Phellodendron chinense ...Two detection methods, real-time fluorescence quantitative PCR (real-time PCR) and loop-mediated ... See full document
9
Development of a Loop-Mediated Isothermal Amplification Method for Detection of Histoplasma capsulatum DNA in Clinical Samples
... treatment of PDH results in mortality rates between 30 and 42% (22–26) in regions where the disease is endemic and where there are underserved populations or limited diagnostic ...amounts of DNA with high ... See full document
7
Clinical evaluation of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Neisseria meningitidis in cerebrospinal fluid.
... agent of bacterial meningitis in ...isolation of bacteria from patients’ cerebrospinal fluid (CSF) is time consuming and some- times yields negative ...methods of detecting Nm have been considered ... See full document
13
Rapid, simple and sensitive detection of Q fever by loop-mediated isothermal amplification of the htpAB gene.
... because of a lack of specific clinical manifestations ...lack of recognition and laboratory testing capacity for Q fever, almost all of the recorded cases of Q fever in China are ... See full document
6
Rapid and sensitive detection of Bordetella bronchiseptica by loop-mediated isothermal amplification (LAMP)
... aetiology of atro- phic rhinitis and bronchopneumonia, which increase inci- dence and severity of respiratory system disease through cooperate with other pathogenic germs, so ...diagnosis of ... See full document
5
A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes.
... Today rapid assays are available that can report infection efficiently, for example by detect- ing anti-HIV antibodies in oral samples—however, the nucleic acid-based method presented here has additional ... See full document
11
Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification
... that of conventional ...a rapid, simple, specific, sensitive and reliable diagnostic protocol that can be applied under field conditions, even at China's trade borders, to prevent ASFV spread into ...ASFV ... See full document
7
Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method
... total of 87 bacteria strains consisting of 30 ...tute of Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia’s Culturebank, Kelantan Public Health laboratory and Institute Medical ... See full document
7
Rapid and sensitive detection of novel avian-origin influenza A (H7N9) virus by reverse transcription loop-mediated isothermal amplification combined with a lateral-flow device.
... deaths. Rapid detection of the novel H7N9 virus is urgently needed to differentiate the disease from other infections, and to facilitate infection control as well as epidemiologic ...transcription ... See full document
7
Rapid and Sensitive Detection of the Main Contaminating Fungus Penicillium restrictum in Jet Fuel using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick
... Loop-mediated isothermal amplification (LAMP) assay is a rapid and specific DNA amplification method described originally by Notomi et ...set of four primers were ... See full document
12
Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by loop-mediated isothermal amplification (LAMP).
... T. brucei subspecies and T. evansi [11]. LAMP is an isothermal DNA amplification method with excellent analytical sensitivity and specificity when employed for the detection of a ... See full document
10
J. bras. pneumol. vol.33 número4 en v33n4a13
... µl of reaction mixture containing 20 mM Tris-HCl (pH ...each of the primers BC3 (5’-TCGACGGCAGAAAGCGTGCC-3’) and BC5 (5’- ...(complement of bases 200266 to 200285) and BC5 (bases 200679 to 200697), ... See full document
6
Programmed Cell Death in Procyclic Form Trypanosoma brucei rhodesiense - Identification of Differentially Expressed Genes during Con A Induced Death
... member of the RACK gene fam- ily which are homologues of the beta sub-unit of G proteins and which are intracellular receptors for activated protein kinase ...death of procyclic T. b. ... See full document
6
Comparison of the efficacies of loop-mediated isothermal amplification, fluorescence smear microscopy and culture for the diagnosis of tuberculosis.
... (specificity of 93.6%). The utility of LAMP as a tool to resolve differences between the culture and smear results is questionable, especially when smear negative samples are ...development of a LAMP ... See full document
5
DESENVOLVIMENTO E OTIMIZAÇÃO DA TÉCNICA LAMP (LOOP-MEDIATED ISOTHERMAL DNA AMPLIFICATION) PARA A IDENTIFICAÇÃO DAS PRINCIPAIS GENOESPÉCIES DO COMPLEXO Borrelia burgdorferi sensu lato MARTA MARIA ALMEIDA DIAS DO NASCIMENTO
... afzelii e B. burgdorferi s.s. e de 0,02pg (10 cópias/µl) para B. garinii, tendo os valores de sensibilidade sido substancialmente mais baixos (30). Outro exemplo é o trabalho publicado este ano (2015) por Zhang e ... See full document
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Phylogeography and taxonomy of Trypanosoma brucei.
... biology of T. brucei and the epidemiology of sleeping sickness [14,15,16,17,18,19,20,21,22, ...distinction of the different parasite forms are still not very well ...parts of eastern ... See full document
11
J. Braz. Chem. Soc. vol.20 número4
... two AB type aromatic protons at d 7.17 (1 H, d, J 8.0Hz, H-6) and 6.96 (1H, d, J 8.0Hz, H-7), one N-methyl group at d 3.74 and one O-methyl group at d 3.76. Also signals of two trisubstituted double bonds at d ... See full document
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