[PDF] Top 20 Four methods of preparing mRNA 5' end libraries using the Illumina sequencing platform.
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Four methods of preparing mRNA 5' end libraries using the Illumina sequencing platform.
... Illumina sequencing libraries were prepared following Meyer & Kircher ...biotin-collected 5 9 ends were subjected to blunt-end repair (100 ng of collected 5 9 ends ... See full document
15
The accuracy, feasibility and challenges of sequencing short tandem repeats using next-generation sequencing platforms.
... for the loci analyzed in this study was over 5-fold higher by the 454- platform compared to Illumina, which resulted in differences between the two NGS strategies in number ... See full document
14
De novo transcriptome sequencing of the Octopus vulgaris hemocytes using Illumina RNA-Seq technology: response to the infection by the gastrointestinal parasite Aggregata octopiana.
... from the hemocytes of 5 sick and 5 healthy octopuses selected from each group was extracted according to the Invitrogen ...DNA. The RNA samples were purified using RNeasy ... See full document
15
Transcriptome sequencing and De Novo analysis of Youngia japonica using the illumina platform.
... for Illumina Sequencing The mRNA-seq library was constructed using an mRNA-Seq Sample Preparation Kit (Cat# RS-930-1001, Illumina ...following the manufacturer’s ... See full document
10
Selection of the temperature of casting the bronzes to plaster moulds
... opinion of the influence of the temperature of casting on the size of the volumetric contraction, the studied grades of the bronze, it was ... See full document
6
Assembly and annotation of the sardine (Sardina pilchardus) transcriptome
... for the assembly. The Trinity de novo and Trinity genome guided assemblies that could be done with either local or end-to-end ...assembling the reads from each tissue, a reference ... See full document
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De novo assembly and transcriptome analysis of the rubber tree (Hevea brasiliensis) and SNP markers development for rubber biosynthesis pathways.
... is the primary source of natural rubber that is native to the Amazon ...rainforest. The singular properties of natural rubber make it superior to and competitive with synthetic rubber ... See full document
14
Differential Gene Expression (DGE) by RNA sequencing analysis and development of software for integrating different DGE methods
... illustrate the potential of the ...designed using four biological replicates with two samples for wild type (WT) and two for the mutant NIK T474D lines (Santos et ...2009). ... See full document
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Recent Progress in the Methods of Genome Sequencing
... amplify the DNA fragments in vitro, and of a fabricated substrate and instrument that performs Pyrosequencing (Dressman et al, 2003) in picolitre-sized wells (Thomas et al, ...2006). The genome is ... See full document
7
Common contaminants in next-generation sequencing that hinder discovery of low-abundance microbes.
... number of whole genome shotgun sequencing libraries processed at different sequencing centers by a standardized protocol and not expected to contain etiologic microbes or be complicated by ... See full document
8
Characterization of X chromosome inactivation using integrated analysis of whole-exome and mRNA sequencing.
... by the consenting staff person. In addition, written consent for the minor under the age of 18 years was obtained from the ...years of age provided written consent at the ... See full document
24
Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing.
... role of microRNAs (miRNAs) as a post-transcriptional gene regulator has been elucidated in a broad range of organisms including domestic ...Characterization of miRNAs in normal tissues is an ... See full document
10
The Impact of the Expansion of the Bolsa Familia Program on the Time Allocation of Youths And Their Parents Lia Chitolina Miguel Nathan Foguel Naercio Menezes-Filho
... As the multinomial model is non-linear, the marginal effect of the treatment in a DID model is not the marginal impact of the interaction between time and treatment, but ... See full document
39
Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.
... captured using the MAGNOTEX-SA kit (Takara Bio) according to the manufacturer’s instructions except for the final ...washing, the cDNA-attached beads were suspended in 100 ml of ... See full document
13
Development and validation of a 20K single nucleotide polymorphism (SNP) whole genome genotyping array for apple (Malus × domestica Borkh).
... Other methods have been developed recently for high- throughput genotyping of eukaryotic genomes using short-read sequencing technologies [39–41], and these techniques have been employed in ... See full document
9
Metagenomic profiling of antibiotic resistance genes and mobile genetic elements in a tannery wastewater treatment plant.
... to the observed divergence of microbial community structure between anaerobic sludge and aerobic ...that the phyla of Synergistetes and Thermotogae (known to be anaerobic bacteria) had higher ... See full document
9
Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.
... FLX platform) and 400 bp (e.g. Titanium platform) of read length were observed in taxonomic identification by their in- silico ...three methods on the Titanium platform allowed ... See full document
6
Qualitative and Quantitative Methods in Libraries (QQML) 5: 211- 220, 2016
... purpose of this paper is to discuss the methodological approaches used in this process, namely the confluence of future studies, action learning and stakeholder analysis, integrating different ... See full document
10
The application of optical measurements for the determination of accuracy of gear wheels casts manufactured in the RT/RP process
... discusses the possibilities of using optical measurements for defining the geometric accuracy of gear wheels casts manufactured in the rapid prototyping ...process. The ... See full document
4
INTERACTIVE SYSTEM FOR ON-LINE CONSULTING
... requirements of employers in Romania 614 ...(knowledge of foreign languages, computer knowledge: Word, Excel, Power Point, Internet, communication and law knowledge of: Labor Code, Tax Code, ... See full document
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