[PDF] Top 20 Microbial contamination in next generation sequencing: implications for sequence-based analysis of clinical samples.
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Microbial contamination in next generation sequencing: implications for sequence-based analysis of clinical samples.
... advantage of a well-controlled study performed by the Genetic European Variation in Health and Disease (GEU- VADIS) consortium ...[13,26]. In their pilot study, ERP000177, RNA from five Ep- ... See full document
6
Quantifying Next Generation Sequencing Sample Pre-Processing Bias in HIV-1 Complete Genome Sequencing
... Currently-used clinical drug resistance tests also focus on a limited number of small genomic ...development of drugs that target different steps in the viral life cycle ...[18]. In ... See full document
14
Common contaminants in next-generation sequencing that hinder discovery of low-abundance microbes.
... assumption in calculating the sensitivity of such approaches is that one microbial read is informative ...two clinical samples for reads not aligning to the human genome, we encountered ... See full document
8
Detection and removal of biases in the analysis of next-generation sequencing reads.
... majority of NGS experiments, regardless of whether they are aimed at DNA or ...RNA. In contrast, the bias of non-hydrolysis is mostly relevant for analyses at the RNA level ...mRNA ... See full document
12
Next-generation sequence analysis of cancer xenograft models.
... Next-generation sequencing (NGS) studies in cancer are limited by the amount, quality and purity of tissue ...samples. In this situation, primary xenografts have proven ... See full document
12
Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.
... similarity in microbial compositions among those ten samples was further examined and ...(MDS) analysis showed that samples from the same environment closely clustered together ... See full document
6
Comprehensive Analysis of Disease-Related Genes in Chronic Lymphocytic Leukemia by Multiplex PCR-Based Next Generation Sequencing.
... CLL sequencing published so far have implemented NGS methods without giving details on the performance of the ...requirements of targeted NGS results using the HaloPlex probe technology from Agilent ... See full document
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Molecular diagnosis of Usher syndrome: application of two different next generation sequencing-based procedures.
... patient samples, which previously turned out to be negative for known mutations in Usher genes as assessed by APEX microarray screening ...[31]. In the analysis, we focused only on the coding ... See full document
10
DNA qualification workflow for next generation sequencing of histopathological samples.
... Histopathological samples are a treasure-trove of DNA for clinical ...quality of DNA can vary depending on the source or extraction method ...qualification of DNA preparations is ... See full document
8
A trial of karyotypic microdissection as an enrichment pathway for next-generation sequencing
... Some of these simplifications encompass whole algorithm categories, while others are specific to certain ...example of a trade-off common to some assemblers is the exclusion of the quality values ... See full document
104
DNase SISPA-next generation sequencing confirms Schmallenberg virus in Belgian field samples and identifies genetic variation in Europe.
... SBV sequence info we obtained was compared to the whole genome sequence that was determined from the virus originally isolated from diseased bovines in ...field samples showed together 16 ... See full document
6
The mosaic architecture of Aeromonas salmonicida subsp. salmonicida pAsa4 plasmid and its consequences on antibiotic resistance
... regions in the three plasmids harboring most of the conjugative transfer genes also displayed the most differences ...inversion of the traLEKBVA locus and the surrounding region seemed to have ... See full document
17
Probing the SELEX process with next-generation sequencing.
... Evolution of Ligands by Exponential Enrichment’’) has been introduced independently in the labs of Larry Gold [1] and Jack Szostak [2] as a novel in vitro selection technique for the ... See full document
10
Correction: A Next-Generation Sequencing Method for Genotyping-by-Sequencing of Highly Heterozygous Autotetraploid Potato.
... Uitdewilligen JGAML, Wolters A-MA, D’hoop BB, Borm TJA, Visser RGF, van Eck HJ (2013) A Next- Generation Sequencing Method for Genotyping-by-Sequencing of Highly Heterozygous Autotetrapl[r] ... See full document
1
Analysis of microbial contamination of gutta-percha points commonly used in clinical practice: a practical approach
... use of the packages of GP points is related to the their ...handling of the boxes in which they are present, even if for different gauges, considerably increases the time of exposure to ... See full document
30
Barriers And Profits Of Distan ce Education In Operations Research Based Decision Analysis
... layer in web-based systems is usually developed as set of HTML (or DHTML) files with server side processing provided by merging HTML with general programming code ...Pages). In practice, this ... See full document
12
Leukemia gene atlas--a public platform for integrative exploration of genome-wide molecular data.
... advances in high-throughput technologies allow to collect unprecedented amounts of genomic, trancriptomic and epigenomic ...be based on genome wide microarray expression data of more than 2 ... See full document
7
Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples
... simulation analysis - Looking to imple- ment a bioinformatics pipeline that allowed manageable and cost effective sequencing conditions, we first carried out an NGS simulation ...ber of human and ... See full document
9
PLoS Computational Biology Conference Postcards from ISMB/ECCB 2011.
... data. In his talk, ...(EHRs) of Danish patients to extract patient-level phenotypic ...earlier, in the 1990s. Fortunately, there are still a few health care providers in the US, such as ... See full document
4
Copy number variation of individual cattle genomes using next-generation sequencing
... confirm in- dividual copy number variants, including aCGH, quantitative PCR (qPCR), and fluorescent in situ hybridization ...test samples and Dominette as the reference for all ...CNVs. Based ... See full document
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