[PDF] Top 20 Negligible immunogenicity of induced pluripotent stem cells derived from human skin fibroblasts.

... rejection of syngeneic mouse iPSCs (miPSCs) following transplantation [17], in which miPSCs derived via the episomal approach were less prone to immune-mediated attack than those generated using viral ... See full document

... series of normal single-cell derived clones by whole genome and exome ...characteristics of the acquired mutations in a monoclonal cell isolated from a healthy individual and subsequently ... See full document

... in pluripotent stem cells and that the protein levels increase during motor neuron ...importance of VAPB levels has been documented in Drosophila neuromuscular ...Over-expression of the ... See full document

... function of the nervous system is maintained via well-coordinated processes both in central and peripheral nervous tissues, which govern the homeostasis of organs/tis- ...Impairments of the nervous ... See full document

... models from GD patients with various genotypes and differentiated iPSc to macrophages, neurons or both (Table ...use of a GD iPSc model for mechanistic studies ...a human neuroglioma cell line (H4) ... See full document

... generation from iPS and E14 cell ...C57-iPS-12 cells showed similar potency in motoneruon ...percentage of both ChAT-positive neurons and Hb9-positive neurons between ALS- iPS-1 and C57-iPS-12 groups ... See full document

... 5% of genes were removed from the data set (Figure S7, ...analysis of mitochondrial gene expression. We found similar proportions of reads coming from mitochondrial genes in adult, IPS ... See full document

... differentiation from hBMMSC-iPSCs. We did so to evaluate the potential of iPSCs for therapeutic ...endothelial cells, such as BMP4, PD98059, Flt3L, SCF, and VEGF, were selected and divided into ... See full document

... modified from a human iPSC hematopoietic differentiation ...stromal cells overgrown for 8–10 days in differentiation media containing 10% fetal bovine serum (FBS), 100 μM monothioglycerol ... See full document

... methylated stem cell-specific DMRs and significantly higher expression, and 17 genes with the hyper-methylated stem cell- specific DMRs and significantly lower expression in iPSCs/ESCs from 22 iPS ... See full document

... clustering of upregulated genes in hiPSCs and donor cells with respect to hESCs further confirmed the proximity of hiPSCs to their corresponding cell of origin (Figure 5) as compared to other ... See full document

... development of retinal neurons from embryonic stem (ES) cell or induced pluripotent stem (iPS) cell cultures ...track stem cell differentiation, fluorescent reporters have ... See full document

... adult human skin-derived fibroblasts using either four retroviral vectors encoding Oct3/4, Sox2, Klf4, and c-Myc, or a single polycistronic Oct3/4-Klf4-Sox2-c-Myc-GFP expressing viral vec- ... See full document

... for human pluripotent stem cells including human embryonic stem cells (hESCs) and human induced pluripotent stem cells ...production ... See full document

... discovery of a simple approach for reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) has revolutionized regenerative medicine ...high-levels ... See full document

... Differentiation of iPSCs in either condition resulted in a rise in the proportion of cells able to fire induced action potentials (iAPs) upon current injection (Figure βA, left) between weeks ... See full document

... bottom of the culture dish using a marker pen. The numbers of positively stained clones derived from HTFs/HPFs were counted and compared between different culture ...number of positive ... See full document

... passage of years, the impact of age on oocytes being consistent with its effect on the risk of congenital abnormalities (Balen, ...contain cells which can be isolated and propagated, and have ... See full document

... iPS Cells: Human induced pluripotent stem cells (iPSCs, cat# SC102A-1) were generated by transducing genetically unmodified human fibroblasts with viruses ... See full document

... and fibroblasts were isolated from neonatal foreskin from healthy individuals from the Children’s Hospital at Columbia University Medical ...trypsinization of keratinocytes and after ... See full document