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[PDF] Top 20 Pichia pastoris Like an Expression System

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Pichia pastoris Like an Expression System

Pichia pastoris Like an Expression System

... that an inorganic phosphate source is commonly added into the feed for the purposes of phosphorous supple- mentation (Torre et ...as an antinutrient factor since it forms insoluble complexes with proteins ... See full document

6

Whole Pichia pastoris yeast expressing measles virus nucleoprotein as a production and delivery system to multimerize Plasmodium antigens.

Whole Pichia pastoris yeast expressing measles virus nucleoprotein as a production and delivery system to multimerize Plasmodium antigens.

... delivery system appears attractive. Here, we evaluated Pichia pastoris yeast as an alternative vaccine production and delivery system for the circumsporozoite protein (CS) of ... See full document

13

Expressão recombinante e caracterização funcional da β-amilase de banana produzida...

Expressão recombinante e caracterização funcional da β-amilase de banana produzida...

... heterologous expression of the β-amylase genes allow us to understand the mechanisms of action of this enzyme present in the pulp of ...heterologous expression in Pichia pastoris in order to ... See full document

60

Applications of recombinant Pichia pastoris in the healthcare industry

Applications of recombinant Pichia pastoris in the healthcare industry

... Pichia pastoris expression system was used to pro- duce functionalized single-chain antibody fragments (scFv) directed against the ED-B domain of the B-fibro- nectin (B-Fn) isoform (Marty et ... See full document

6

Production of chitin-glucan complex by Pichia pastoris

Production of chitin-glucan complex by Pichia pastoris

... Pichia pastoris was initially developed in 1970 by a North American oil company, named Philips Petroleum Company, for production of single-cell protein (SCP) as high-protein animal feed (Cereghino and ... See full document

74

Expression of enzymatically inactive wasp venom phospholipase A1 in Pichia pastoris.

Expression of enzymatically inactive wasp venom phospholipase A1 in Pichia pastoris.

... in expression plasmid pPICZaA after the strong methanol-inducible alcohol oxidase 1 (AOX1) promoter and in-frame with the a-mating factor pre-propeptide for secretion from the ...P. pastoris strains: wild ... See full document

12

HATL5: a cell surface serine protease differentially expressed in epithelial cancers.

HATL5: a cell surface serine protease differentially expressed in epithelial cancers.

... a Pichia Expression Kit (Invitrogen, Life Technologies, Grand Island, ...remove an XhoI site using the Agilent Quick-Change II TM site directed mutagenesis kit (Agilent Technologies, ...domain. ... See full document

11

Biosynthesis optimization of STEAP1 protein in Pichia pastoris X33 MUT+

Biosynthesis optimization of STEAP1 protein in Pichia pastoris X33 MUT+

... its expression is ...differential expression of STEAP1 in tissues, associated with its localization, suggests its potential use as a target for cancer ...in Pichia pastoris X33 Mut + . The use ... See full document

68

Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris

Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris

... electrophoresis system (Amersham Biosciences, United ...transfer system (Amersham Biosciences, United Kingdom) immersed in transfer buffer ...in an stove at 25 °C for 1 hour, stained with Ponceau S ... See full document

8

Expressão de uma quitinase de  Violaceum em Pichia Pastoris: purificação e caracterização parcial da proteína recombinante.

Expressão de uma quitinase de Violaceum em Pichia Pastoris: purificação e caracterização parcial da proteína recombinante.

... the expression of proteins from a variety of sources, including plants, animals and other ...is an important structural component found in the crustacean shells, in the peritrophic membrane of insect guts ... See full document

110

Braz. J. Chem. Eng.  vol.21 número2

Braz. J. Chem. Eng. vol.21 número2

... Pichia pastoris (strain GS115) was transformed by the electroporation method with purified expression plasmids rPsd1/pPIC9, previously linearized with SalI (Gibco), favoring vector integration at the ... See full document

10

Avaliação da atividade antimicrobiana das defensinas recombinantes de ervilha (drr230a) e café (cd1) produzidas em Pichia pastoris

Avaliação da atividade antimicrobiana das defensinas recombinantes de ervilha (drr230a) e café (cd1) produzidas em Pichia pastoris

... represent an important innate component from plant defense response against pathogens and are expressed in various plant tissues, such as leaves, tubers, flowers, pods and ...yeast expression vector and ... See full document

112

Produção em Pichia pastoris de uma quitinase de feijãodecorda com atividade antifúngica

Produção em Pichia pastoris de uma quitinase de feijãodecorda com atividade antifúngica

... the expression vectors pET32a(+) and pPICZαA, for heterologous expression in Escherichia coli and Pichia pastoris, ...P. pastoris, the recombinant cowpea chitinase was secreted in a ... See full document

156

Expressão heteróloga de defensina de ervilha (pisum sativum) em pichia pastoris e avaliação da atividade biológica contra fungos de impotância agronômica

Expressão heteróloga de defensina de ervilha (pisum sativum) em pichia pastoris e avaliação da atividade biológica contra fungos de impotância agronômica

... 56 KOBAYASHI, Y.; TAKASHIMA, H.; TAMAOKI, H.; KYOGOKU, Y.; LAMBERT, P.; KURODA, H.; CHINO, N.; WATANABE, T. X.; KIMURA, T.; SAKAKIBARA, S.; MORODER, L., The Cystine-Stabilized Alpha-Helix - a Common Structural Motif of ... See full document

62

Analysis of glycosylation heterogeneity in antibody production by Pichia pastoris

Analysis of glycosylation heterogeneity in antibody production by Pichia pastoris

... The protein of interest in this study is Anti-ED-B Fibronectin antibody fragment. The fibronectin is a universal cell-adhesion molecule which has an alternative splicing. This splice variants is ED-B fibronectin. ... See full document

95

Expression of MdL1 and MdL2 in Pichia pastoris

Expression of MdL1 and MdL2 in Pichia pastoris

... in Pichia pastoris , purified and ...have an acidic pH optimum ...towards an even more acidic value, pH ...is an intrinsic property of MdL1 and MdL2, whereas pH optimum shifts are ... See full document

9

Expressão e caracterização de uma protease de interesse biotecnológico clonada da...

Expressão e caracterização de uma protease de interesse biotecnológico clonada da...

... circulatory system and are promising for the treatment of a variety of hemostatic ...the expression of a serine protease from Crotalus durissus collilineatus (Collineina- 1) in Pichia ... See full document

54

Improvement of STEAP 1 Biosynthesis from Pichia pastoris X33 cells under an optimized feeding strategy

Improvement of STEAP 1 Biosynthesis from Pichia pastoris X33 cells under an optimized feeding strategy

... The deep knowledge of STEAP1 structure and function is crucial for the development of new molecules with therapeutic and clinical applications. Thereby, several modifications in the STEAP1 structure were reported and ... See full document

102

Bao Zhen Feng, Peiqian Li

Bao Zhen Feng, Peiqian Li

... activity expression of PCLAC2, expression vector pPIC9K with the Pichia pastoris expression system was ...with an apparent molecular weight ... See full document

7

Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

... the expression levels of 8-oxoguanine DNA glycosylase (OGG1), Glutathione Reductase (GLR), factor of response to heat stress (HSF-1) and heat shock protein 70 (HSP-70), the levels of all pro- teins were measured ... See full document

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