[PDF] Top 20 Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
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Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
... Phenyl- and CM-Sepharose were obtained from Pharmacia Fine Chemicals (Uppsala, Sweden); ethylenediamino tetratacetic acid (EDTA), dithiotreitol (DTT), ammonium sulfate, rabbit GAPDH, agarose, trietanolamine, ... See full document
6
Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi
... presence of marker enzymes (pyruvate kinase, hexokinase and alanine aminotrans- ferase), according to Uttaro and Opperdoes ...role of SOD is to detoxify endogenous superoxide anions and ... See full document
6
Looking for combination of benznidazole and Trypanosoma cruzi- triosephosphate isomerase inhibitors for Chagas disease treatment
... 2 and Bnz, independently, have concen- tration-dependent effects on epimastigotes of Tulahuen 2 strain, DTU Tc VI, with an IC 50 of ...12.0 and 7.0 µM, re- spectively. The combination ... See full document
8
Partial Purification of Milk Clotting Protease
... extracts of these flowers have been employed for ages in Portugal and bordering regions of Spain for the production of traditional cheeses from raw ewe’s milk (Roa et ...1999) and/or ... See full document
9
Canine trypanosomiasis: etiology of infection and implications for public health
... presence of active ...treatment and the elimination of infection; this can imply interpretation problems in these assays, which would impede the determination of infection status in each ... See full document
23
Functional characterization of 8-oxoguanine DNA glycosylase of Trypanosoma cruzi.
... removal of the 8-oxodG base. This enzyme acts both in the nucleus and the mitochondria ...forms of cancer, diabetes and Huntington disease ...thaliana and Homo sapiens [9,16,17,18,19]. ... See full document
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Swimming against the current: genetic vaccination against Trypanosoma cruzi infection in mice
... model of infec- tion. During the chronic phase of the disease, most in- During the chronic phase of the disease, most in- dividuals have strong CMI to parasite antigens (Morato et ...depression ... See full document
7
An Investigation on the Ecology of Triatoma vitticeps (Stal, 1859) and its Possible Role in the Transmission of Trypanosoma cruzi, in the Locality of Triunfo, Santa Maria Madalena Municipal District, State of Rio de Janeiro, Brazil
... ity of Triunfo, 2nd District of Santa Maria Madalena municipal district, which has the higher incidence of the triatomine infected with ...T. cruzi- like (Ferreira et al. 1986). The aim ... See full document
8
Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli
... sequenced and transferred to the bacterial expression vector pET21a (data not ...kinetics of urate oxidase expression, a colony of ...mid-log and induced with IPTG for 0, 15, 30, 60, 90, ... See full document
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Comparative Analysis by Polymerase Chain Reaction Amplified Minicircles of Kinetoplast DNA of a Stable Strain of Trypanosoma cruzi from São Felipe, Bahia, its Clones and Subclones: Possibility of Predominance of a Principal Clone in this Area
... Molecular characterization of one stable strain of Trypanosoma cruzi, the 21 SF, representative of the pattern of strains isolated from the endemic area of São ... See full document
8
Junei Kinjo3, Simón Hernández-Ortega
... brasiliense and Mammea americana (Clusiaceae) are two trees from the tropical rain forests of the American ...extracts of these species. Several mammea-type coumarins, triterpenoids and ... See full document
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Estudos funcionais de genes envolvidos na manutenção da estabilidade genômica : caracterização de gene hHC1 humano e da DNA polimerqase -PAK de Trypanosoma cruzi.
... Cada vez torna-se mais evidente a importância de estudos envolvendo fatores associados a sistemas que “protegem” o material genético, a fim de evitar doenças genéticas ou desordens que podem resultar na morte do ... See full document
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Analysis of the Paracoccidioides brasiliensis triosephosphate isomerase suggests the potential for adhesin function
... molecules, and the outcome of such colonization depends largely on the receptor/ligand interac- tions between the host cells and the ...infection of P. brasiliensis originates in the lung ... See full document
8
Substrate-Induced Dimerization of Engineered Monomeric Variants of Triosephosphate Isomerase from Trichomonas vaginalis.
... deletion of loop 3 are unstable and present poor catalytic activity [10, 11, ...dissociation and unfolding reac- tions of TvTIM1 indicate that dissociation contributes to only 18% of ... See full document
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Trypanosoma cruzi: Inhibition of
... Trypanosoma cruzi, the flagellate parasite that causes Chagas disease, possesses an enzyme similar to the lac- tate dehydrogenase isozyme-x (LDH-C4) from mammalian spermatozoa (Gerez de Burgos et ...T. ... See full document
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MATERIALS AND METHODS Production, extraction and partial purification of EPS
... higher and it is reached faster in the medium containing only ...falling of the cellular concentration could be explained by the depletion of the energy source, and the posterior cellular ... See full document
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The level of ascorbate peroxidase is enhanced in benznidazole-resistant populations of Trypanosoma cruzi and its expression is modulated by stress generated by hydrogen peroxide
... silico and phylogenetic analyses of the TcAPX gene - In silico analyses were conducted on a local server using in-house copies of the appropriate databases and ...sequence of TcAPX ... See full document
10
Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
... body of evidence in mammalian cells indicates that secreted vesicles can be used to mediate intercellular communication processes by transferring various bioactive molecules, including mRNAs and ...whether ... See full document
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Morphobiological Characterization of Trypanosoma cruzi Chagas, 1909 and its Distinction from other Trypanosomes
... site of this parasite in mammals has been con- vincingly demonstrated (D’Alessandro-Bacigalupo & Saravia ...T. cruzi, can invade the hemocele, as well as the salivary glands, where large number ... See full document
6
Partial purification and characterization of a bacteriocin produced by Enterococcus faecium 130 isolated from mozzarella cheese
... group of the lactic acid bacteria (LAB) and have ambiguous significance in foods with both beneficial and detrimental ...production of antimicrobials, synthesize flavor compounds, and ... See full document
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