[PDF] Top 20 Quantitative real-time PCR for the clinical detection of Helicobacter pylori
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Quantitative real-time PCR for the clinical detection of Helicobacter pylori
... only the semi-quantitative de- termination of infection, and both require experienced pro- fessionals for their execution and the interpretation of results (Christensen et ...On ... See full document
4
Real-Time PCR for Measles Virus Detection on Clinical Specimens with Negative IgM Result in Morocco.
... number of suspected measles cases each year, in spite of the high vaccination coverage reported for the routine dose as well as for the mass vaccina- tion campaign in 2008 ...Through ... See full document
10
Quantitative detection of Borrelia burgdorferi sensu lato in erythema migrans skin lesions using internally controlled duplex real time PCR.
... duplex quantitative real time PCR assay targeting the Borrelia single copy 16S rRNA gene and the single copy human RNAseP ...monitor the efficiency of DNA ... See full document
9
Fast detection of deletion breakpoints using quantitative PCR
... to the manufacturer’s ...USA). The primers used are listed in Supplementary Tables S1-S3. Real-time quantitative PCR (qPCR) was done using an MxPro3000 apparatus (Stratagene, ... See full document
5
REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN
... level of detection was ...with the CT value of 36.6 ± 1.6. The real-time PCR assay we developed was approximately 1000-fold more sensitive than conventional ... See full document
9
Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications.
... determine the suitability of our new ...range of conditions, we tested the performance of the 122018 and 266152 assays across several criteria; accuracy, specificity, precision, ... See full document
9
Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction.
... Real-time PCR has been implemented in clinical laboratories for the detection of bacterial meningitis ...burdens of disease, laboratories are required to test ... See full document
7
Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus
... in the genomic content of the Msp I satellite DNA ...number of nematodes and fluorescence detection, this observation precludes the use of the Msp I repetitive ... See full document
7
Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria
... that the LoD is of great interest to de- termine treatment endpoints, the lowest number of p/μL that could be detected in PCR assays was ...examination, the parasitaemia counts ... See full document
6
DEVELOPMENT OF CONVENTIONAL AND REAL-TIME MULTIPLEX PCR ASSAYS FOR THE DETECTION OF NOSOCOMIAL PATHOGENS Deepa Anbazhagan
... Real time PCR is a technique used to monitor the progress of a PCR reaction in real ...At the same time, a relatively small amount of PCR ... See full document
11
In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
... validation of the assay, the international panel cited above was used in qPCR reactions to construct standard curves of HBV DNA or HCV RNA in the following concentrations: HBV 7 or HCV ... See full document
6
Development and optimisation of a group-specific real-time RT-PCR assay for the broad detection of the Simbu serogroup orthobunyaviruses
... within the genus Orthobunyavirus belongs to the family Peribunyaviridae (Adams, Lefkowitz, King, Harrach, Harrison, Knowles, Kropinski, Krupovic, Kuhn, Mushegian, Nibert, Sabanadzovic, Sanfaçon, Siddell, ... See full document
112
A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks
... multaneous detection of ...poultry clinical samples. For the development of this as- say, we first implemented a MS real time PCR test, based on the ... See full document
6
Development of a real-time PCR assay for the detection of the golden mussel (Limnoperna fortunei, Mytilidae) in environmental samples
... for the management of invasive species is precisely that in which it is less detectable, ...during the early stages of ...control the golden mussel depend heavily on a means for its ... See full document
5
Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR
... herbs. The Lancet 1993; ...Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant ...survey of herbal medicines from markets in China using DNA ... See full document
9
Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR
... extend the use of a primer set commonly involved in the characterization of soil fungal community composition in the literature, with quantification of the soil fungal ... See full document
13
QUANTITATIVE REAL-TIME PCR (Q-PCR) FOR SPUTUM SMEAR DIAGNOSIS OF PULMONARY TUBERCULOSIS AMONG PEOPLE WITH HIV/AIDS
... a clinical suspicion of ...assess the accuracy of a diagnostic test, conducted on 140 sputum specimens from 140 patients living with HIV/AIDS with a clinical suspicion of PTB, ... See full document
4
Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus.
... considered the 5–6% genomic difference between genotypes and predicted that the significant number of mismatches would impair the successful detection of many contemporary ... See full document
15
Novel set of real-time PCR primers for simultaneous detection of Liberibacter
... by the bacterium “Candi- datus Liberibacter ...and the non-cultivability of the bacterium has severely hampered studies on the ...pathogen. The 16S rDNA gene is a ... See full document
8
Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... using the following cycling parameters: 50°C for 2 min, 95°C for 10 min, 45 cycles at 95°C for 2 min, 62°C for 30 seconds and 72°C for 30 seconds; the data were collected in the last (extension) ... See full document
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