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[PDF] Top 20 Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.

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Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.

Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.

... Quantitative real-time reverse transcription polymerase chain reaction (qPCR), a sensitive technique for gene expression analysis, depends on the stability of the reference genes used ... See full document

10

Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).

Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).

... evaluate gene expression levels and obtain more accurate quantitative real-time RT-PCR (qRT-PCR) data, normalization relative to reliable reference gene(s) ... See full document

9

Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.

Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.

... transcription PCR (RT-qPCR) is a preferred method for rapid and accurate quantification of gene expression ...accurate normalization though the use of reference ...single reference ... See full document

14

Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.

Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.

... of gene expression [16, 23, ...expression under different stress conditions has not been accomplished ...yet. In the present study, a comprehensive gene expression ... See full document

20

Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean

Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean

... the gene of interest with reference genes, whose expression does not change under different experimental ...best reference genes for a certain organism or experimental condition ... See full document

8

Selection and assessment of reference genes for quantitative PCR normalization in migratory locust Locusta migratoria (Orthoptera: Acrididae).

Selection and assessment of reference genes for quantitative PCR normalization in migratory locust Locusta migratoria (Orthoptera: Acrididae).

... involved in citrate cycle and metabolic pathways, ...best reference gene for adult stage and insecticide ...stable in larvae stage followed by EF1a and ...experimental conditions, SDH ... See full document

16

Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.

Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.

... stable reference genes were not identical in all the sets of samples under ...stability in samples under abiotic stress conditions and once again, SAMDC was the ... See full document

12

Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).

Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).

... years. Gene expression analysis is an efficient method to explore the possible regulatory mechanisms underlying these ...of reference genes is required for transcript level normalization under ... See full document

16

Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.

Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.

... changes in gene expression induced by abiotic stress ...both time consuming and labor ...This, in conjunction with the low throughput aspect of northern blotting, restricts its ... See full document

10

Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.

Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.

... Real time quantitative reverse transcription-PCR (qRT-PCR) is increasingly used in gene expression analysis owing to its simple, reproducible and high-throughput ... See full document

10

Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.

Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.

... appropriate reference genes is essential to reverse transcription quantitative real-time PCR (RT-qPCR), which is key to many studies involving gene expression ...analysis. ... See full document

11

Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology

Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology

... residues in a specific and con- served pattern in two domains at the N- and C- ter- minals, clones ...changes in the spacer (central) region of the protein ...involved in protein-protein and ... See full document

6

Loss of function in Mlo orthologs reduces susceptibility of pepper and tomato to powdery mildew disease caused by Leveillula taurica.

Loss of function in Mlo orthologs reduces susceptibility of pepper and tomato to powdery mildew disease caused by Leveillula taurica.

... detailed time points (Figure 5A and Figure ...cluster time points into four ...hpi. In this stage fungal spores landed on the leaf surface and started the germination, defined as fungal germination ... See full document

14

Thiol/Disulfide system plays a crucial role in redox protection in the acidophilic iron-oxidizing bacterium Leptospirillum ferriphilum.

Thiol/Disulfide system plays a crucial role in redox protection in the acidophilic iron-oxidizing bacterium Leptospirillum ferriphilum.

... oxidative stress and how they preserve the thiol/disulfide ...out in extremely acidic conditions (,pH 1) where extraordinarily high concentrations of soluble iron and heavy metals are present ... See full document

10

Integrated Metabolo-Transcriptomics Reveals Fusarium Head Blight Candidate Resistance Genes in Wheat QTL-Fhb2.

Integrated Metabolo-Transcriptomics Reveals Fusarium Head Blight Candidate Resistance Genes in Wheat QTL-Fhb2.

... conducted in a randomized complete block, with two genotypes (R-RIL and S-RIL), two inoculations (pathogen = P and mock = M) and five replications per ...ground in liquid nitrogen using pre- chilled mortar ... See full document

27

Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in flavonoid biosynthetic pathway.

Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in flavonoid biosynthetic pathway.

... step in the flavonoid biosynthetic pathway. In this study, the cDNA (FhCHS1) encoding CHS from Freesia hybrida was suc- cessfully isolated and ...found in the deduced amino acid sequence of ...role ... See full document

18

Selection of suitable housekeeping genes for real-time quantitative PCR in CD4(+) lymphocytes from asthmatics with or without depression.

Selection of suitable housekeeping genes for real-time quantitative PCR in CD4(+) lymphocytes from asthmatics with or without depression.

... implicated in such diseases and provide added insight into the underlying pathophysiological ...mechanisms. Real-time quantitative PCR (qPCR) is a useful technique for acquiring the ... See full document

9

Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.

Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.

... these selection tools, results regarding best reference genes for ovary and uterus samples across reproductive stages were very similar with the three ...methods. In ovary, TBP was the gene ... See full document

9

Acute exposure of apigenin induces hepatotoxicity in Swiss mice.

Acute exposure of apigenin induces hepatotoxicity in Swiss mice.

... especially in short term acute toxicity studies where the full phenotypic signs and symptoms may have not been fully developed ...[18]. In the present study, 48 differentially regulated genes were ... See full document

11

Molecular basis of isoprenoid biosynthesis in Dunaliella salina

Molecular basis of isoprenoid biosynthesis in Dunaliella salina

... Figure 1. Dunaliella salina cells in different culture conditions. A) Green cell from a non-stressed culture; B) Stressed cell turning orange; C) Orange cell from a culture exposed to nutrient ... See full document

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